Novel cell and tissue acquisition system (CTAS): Microdissection of live and frozen brain tissues

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Kudo, Lili C ^a   Vi, Nancy ^a   Ma, Zhongcai ^a,b   Fields, Tony ^c   Avliyakulov, Nuraly K ^c   Haykinson, Michael J ^c   Bragin, Anatol ^a,c   Karsten, Stanislav L ^a  

^a NeuroInDx Inc   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RNA;

ACCURACY; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BRAIN REGION; BRAIN TISSUE; CELL AND TISSUE ACQUISITION SYSTEM; CELL CULTURE; CELL DAMAGE; CELL ISOLATION; CELL POPULATION; CELL VIABILITY; COST EFFECTIVENESS ANALYSIS; DISPOSABLE CAPILLARY UNIT; DISPOSABLE EQUIPMENT; DNA ISOLATION; LABORATORY DEVICE; LASER; MICROARRAY ANALYSIS; MICRODISSECTION; MOUSE; NONHUMAN; PROCEDURES CONCERNING CELLS; PROTEIN ISOLATION; PROTEOMICS; RAT; RNA ISOLATION; WESTERN BLOTTING;

ANIMALS; BRAIN; FREEZING; MICE; MICE, INBRED C57BL; MICRODISSECTION; RATS; RATS, WISTAR;

RATTUS;

EID: 84864266958     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041564     Document Type: Article

References (50)

1. Choudhary J, Grant SG, (2004) Proteomics in postgenomic neuroscience: the end of the beginning. Nat Neurosci 7: 440-445.
2. Geschwind DH, (2000) Mice, microarrays, and the genetic diversity of the brain. Proc Natl Acad Sci U S A 97: 10676-10678.
3. Becker I, Becker KF, Rohrl MH, Minkus G, Schutze K, et al. (1996) Single-cell mutation analysis of tumors from stained histologic slides. Lab Invest 75: 801-807.
4. de With A, Greulich KO, (1995) Wavelength dependence of laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. J Photochem Photobiol B 30: 71-76.
5. Srinivasan R, (1986) Ablation of polymers and biological tissue by ultraviolet lasers. Science 234: 559-565.
6. Schutze K, Lahr G, (1998) Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 16: 737-742.
7. Schutze K, Posl H, Lahr G, (1998) Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine. Cell Mol Biol (Noisy-le-grand) 44: 735-746.
8. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, et al. (1996) Laser capture microdissection. Science 274: 998-1001.
9. Fend F, Emmert-Buck MR, Chuaqui R, Cole K, Lee J, et al. (1999) Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis. Am J Pathol 154: 61-66.
10. Simone NL, Bonner RF, Gillespie JW, Emmert-Buck MR, Liotta LA, (1998) Laser-capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet 14: 272-276.
11. Atkins N, Miller CM, Owens JR, Turek FW, (2011) Non-laser capture microscopy approach for the microdissection of discrete mouse brain regions for total RNA isolation and downstream next-generation sequencing and gene expression profiling. J Vis Exp. (57), e3125 DOI:10.3791/3125.
12. Ginsberg SD, Hemby SE, Mufson EJ, Martin LJ, (2006) Cell and Tissue Microdissection in Combination with Genomic and Proteomic Applications Neuroanatomical Tract-Tracing 3. In: Zaborszky L, Wouterlood FG, Lanciego JL, editors: Springer US. pp. 109-141.
13. Decarlo K, Emley A, Dadzie OE, Mahalingam M, (2011) Laser capture microdissection: methods and applications. Methods Mol Biol 755: 1-15.
14. Espina V, Heiby M, Pierobon M, Liotta LA, (2007) Laser capture microdissection technology. Expert Rev Mol Diagn 7: 647-657.
15. Ohmi K, Kudo LC, Ryazantsev S, Zhao HZ, Karsten SL, et al. (2009) Sanfilippo syndrome type B, a lysosomal storage disease, is also a tauopathy. Proc Natl Acad Sci U S A 106: 8332-8337.
16. Standaert DG, (2005) Applications of laser capture microdissection in the study of neurodegenerative disease. Arch Neurol 62: 203-205.
17. Clarke L, van der Kooy D, (2011) The adult mouse dentate gyrus contains populations of committed progenitor cells that are distinct from subependymal zone neural stem cells. Stem Cells 29: 1448-1458.
18. Eisenstein M, (2006) Cell sorting: divide and conquer. Nature 441: 1179-1185.
19. Etchevers H, (2011) Primary culture of chick, mouse or human neural crest cells. Nat Protoc 6: 1568-1577.
20. Kopper O, Giladi O, Golan-Lev T, Benvenisty N, (2010) Characterization of gastrulation-stage progenitor cells and their inhibitory crosstalk in human embryoid bodies. Stem Cells 28: 75-83.
21. Tofighi R, Moors M, Bose R, Ibrahim WN, Ceccatelli S, (2011) Neural stem cells for developmental neurotoxicity studies. Methods Mol Biol 758: 67-80.
22. Mikulowska-Mennis A, Taylor TB, Vishnu P, Michie SA, Raja R, et al. (2002) High-quality RNA from cells isolated by laser capture microdissection. Biotechniques 33: 176-179.
23. Wang WZ, Oeschger FM, Lee S, Molnar Z, (2009) High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection. BMC Mol Biol 10: 69.
24. Kerman IA, Buck BJ, Evans SJ, Akil H, Watson SJ, (2006) Combining laser capture microdissection with quantitative real-time PCR: effects of tissue manipulation on RNA quality and gene expression. J Neurosci Methods 153: 71-85.
25. Mercer EH, (1963) A SCHEME FOR SECTION STAINING IN ELECTRON MICROSCOPY. Journal of the Royal Microscopical Society 81: 179-186.
26. Abramoff MD, Magalhaes PJ, Ram SJ, (2004) "Image Processing with ImageJ" Biophotonics International 11: 36-42.
27. Mosessian S, Avliyakulov NK, Mulholland DJ, Boontheung P, Loo JA, et al. (2009) Analysis of PTEN complex assembly and identification of heterogeneous nuclear ribonucleoprotein C as a component of the PTEN-associated complex. J Biol Chem 284: 30159-30166.
28. Karsten SL, Kudo LC, Jackson R, Sabatti C, Kornblum HI, et al. (2003) Global analysis of gene expression in neural progenitors reveals specific cell-cycle, signaling, and metabolic networks. Dev Biol 261: 165-182.
29. Brewer GJ, Torricelli JR, (2007) Isolation and culture of adult neurons and neurospheres. Nat Protoc 2: 1490-1498.
30. Ben-Hur T, Rogister B, Murray K, Rougon G, Dubois-Dalcq M, (1998) Growth and fate of PSA-NCAM+ precursors of the postnatal brain. J Neurosci 18: 5777-5788.
31. Lendahl U, Zimmerman LB, McKay RD, (1990) CNS stem cells express a new class of intermediate filament protein. Cell 60: 585-595.
32. Williams BP, Park JK, Alberta JA, Muhlebach SG, Hwang GY, et al. (1997) A PDGF-regulated immediate early gene response initiates neuronal differentiation in ventricular zone progenitor cells. Neuron 18: 553-562.
33. Morrison RS, de Vellis J, (1981) Growth of purified astrocytes in a chemically defined medium. Proc Natl Acad Sci U S A 78: 7205-7209.
34. Lein ES, Hawrylycz MJ, Ao N, Ayres M, Bensinger A, et al. (2007) Genome-wide atlas of gene expression in the adult mouse brain. Nature 445: 168-176.
35. Ng L, Bernard A, Lau C, Overly CC, Dong HW, et al. (2009) An anatomic gene expression atlas of the adult mouse brain. Nat Neurosci 12: 356-362.
36. Liu A, (2010) Laser capture microdissection in the tissue biorepository. J Biomol Tech 21: 120-125.
37. Harsch M, Bendrat K, Hofmeier G, Branscheid D, Niendorf A, (2001) A new method for histological microdissection utilizing an ultrasonically oscillating needle: demonstrated by differential mRNA expression in human lung carcinoma tissue. Am J Pathol 158: 1985-1990.
38. Ibrahim SF, van den Engh G, (2003) High-speed cell sorting: fundamentals and recent advances. Curr Opin Biotechnol 14: 5-12.
39. Lobo MK, Karsten SL, Gray M, Geschwind DH, Yang XW, (2006) FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brains. Nat Neurosci 9: 443-452.
40. Espina V, Wulfkuhle JD, Calvert VS, VanMeter A, Zhou W, et al. (2006) Laser-capture microdissection. Nat Protoc 1: 586-603.
41. Goldsworthy SM, Stockton PS, Trempus CS, Foley JF, Maronpot RR, (1999) Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol Carcinog 25: 86-91.
42. Su JM, Perlaky L, Li XN, Leung HC, Antalffy B, et al. (2004) Comparison of ethanol versus formalin fixation on preservation of histology and RNA in laser capture microdissected brain tissues. Brain Pathol 14: 175-182.
43. Kudo LC, Parfenova L, Vi N, Lau K, Pomakian J, et al. (2010) Integrative gene-tissue microarray-based approach for identification of human disease biomarkers: application to amyotrophic lateral sclerosis. Hum Mol Genet 19: 3233-3253.
44. Craven RA, Banks RE, (2001) Laser capture microdissection and proteomics: possibilities and limitation. Proteomics 1: 1200-1204.
45. Vercauteren FG, Flores G, Ma W, Chabot JG, Geenen L, et al. (2007) An organelle proteomic method to study neurotransmission-related proteins, applied to a neurodevelopmental model of schizophrenia. Proteomics 7: 3569-3579.
46. Cameron HA, Woolley CS, McEwen BS, Gould E, (1993) Differentiation of newly born neurons and glia in the dentate gyrus of the adult rat. Neuroscience 56: 337-344.
47. Gage FH, Coates PW, Palmer TD, Kuhn HG, Fisher LJ, et al. (1995) Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc Natl Acad Sci U S A 92: 11879-11883.
48. Gage FH, Kempermann G, Palmer TD, Peterson DA, Ray J, (1998) Multipotent progenitor cells in the adult dentate gyrus. J Neurobiol 36: 249-266.
49. Gould E, Gross CG, (2002) Neurogenesis in adult mammals: some progress and problems. J Neurosci 22: 619-623.
50. Paxinos GaF, K.B.J (2001) The mouse Brain in Stereotaxic Coordinates. San Diego: Academic Press.