High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection

BMC Molecular Biology

Volumn 10, Issue , 2009, Pages

  Wang, Wei Zhi ^a   Oeschger, Franziska M ^a   Lee, Sheena ^a   Molnár, Zoltán ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN REGION; CONTROLLED STUDY; DNA FRAGMENTATION; FEMALE; FROZEN SECTION; GENE AMPLIFICATION; GENE EXPRESSION PROFILING; INTERMETHOD COMPARISON; LASER CAPTURE MICRODISSECTION; MEMBRANE STABILIZATION; MICROARRAY ANALYSIS; MOUSE; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN ISOLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TISSUE SECTION; ANIMAL; BRAIN; BRAIN LEVEL; CRYOPRESERVATION; CYTOLOGY; DNA MICROARRAY; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; LOW LEVEL LASER THERAPY; METHODOLOGY; MICRODISSECTION; ULTRAMICROTOMY;

ANIMALS; BRAIN; BRAIN CHEMISTRY; CRYOPRESERVATION; CRYOULTRAMICROTOMY; LASER THERAPY; MICE; MICRODISSECTION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA;

EID: 67651232885     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-10-69     Document Type: Article

References (22)

1. Emmert-Buck MR Bonner RF Smith PD Chuaqui RF Zhuang Z Goldstein SR Weiss RA Liotta LA Laser capture microdissection Science 1996, 274:998-1001. 10.1126/science.274.5289.998 8875945
2. Kölble K The LEICA microdissection system: Design and applications J Mol Med 2000, 78:B24-B25.
3. Micke P Ostman A Lundeberg J Ponten F Laser-assisted cell microdissection using the PALM system Methods Mol Biol 2005, 293:151-166. 16028418
4. Clément-Ziza M Munnich A Lyonnet S Jaubert F Besmond C Stabilization of RNA during laser capture microdissection by performing experiments under argon atmosphere or using ethanol as a solvent in staining solutions RNA 2008, 14:2698-704. 2590969 18945804 10.1261/ rna.1261708
5. Kerman IA Buck BJ Evans SJ Akil H Watson SJ Combining laser capture microdissection with quantitative real-time PCR: Effects of tissue manipulation on RNA quality and gene expression J Neurosci Methods 2006, 153:71-85. 10.1016/j.jneumeth.2005.10.010 16337273
6. Fleige S Pfaffl MW RNA integrity and the effect on the real-time qRT-PCR performance Mol Aspects Med 2006, 27:126-139. 10.1016/j.mam.2005.12.003 16469371
7. Wang H Owens JD Shih JH Li MC Bonner RF Mushinski JF Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA BMC Genomics 2006, 7:97. 1513394 16643667 10.1186/1471-2164-7-97
8. Kube DM Savci-Heijink CD Lamblin AF Kosari F Vasmatzis G Cheville JC Connelly DP Klee GG Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer BMC Mol Biol 2007, 21:8-25.
9. Vincent VAM Devoss JJ Ryan HS Murphy GM Jr Analysis of neuronal gene expression with laser capture microdissection J Neurosci Res 2002, 69:578-586. 10.1002/jnr.10329 12210823
10. Kamme F Salunga R Yu J Tran DT Zhu J Luo L Bittner A Guo HQ Miller N Wan J Erlander M Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity J Neurosci 2003, 23:3607-15. 12736331
11. Lombardino AJ Hertel M Li XC Haripal B Martin-Harris L Pariser E Nottebohm F Expression profiling of intermingled long-range projection neurons harvested by laser capture microdissection J Neurosci Methods 2006, 157:195-207. 10.1016/j.jneumeth.2006.04.026 16750569
12. Araki KY Sims JR Bhide PG Dopamine receptor mRNA and protein expression in the mouse corpus striatum and cerebral cortex during pre- and postnatal development Brain Res 2007, 1156:31-45. 1994791 17509542 10.1016/j.brainres.2007.04.043
13. Fernández-Medarde A Porteros A de las Rivas J Núñez A Fuster JJ Santos E Laser microdissection and microarray analysis of the hippocampus of Ras-GRF1 knockout mice reveals gene expression changes affecting signal transduction pathways related to memory and learning Neuroscience 2007, 146:272-85. 10.1016/ j.neuroscience.2007.01.022 17321057
14. Paulsen SJ Larsen LK Jelsing J Janssen U Gerstmayer B Vrang N Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays J Neurosci Methods 2009, 177:87-93. 10.1016/j.jneumeth.2008.09.024 18955080
15. Arimatsu Y Ishida M Kaneko T Ichinose S Omori A Organization and development of corticocortical associative neurons expressing the orphan nuclear receptor Nurr1 J Comp Neurol 2003, 466:180-96. 10.1002/cne.10875 14528447
16. Hoerder-Suabedissen A Wang WZ Lee S Davies KE Goffinet AM Rakic S Parnavelas J Reim K Nicolic M Paulsen O Molnár Z Novel markers reveal subpopulations of subplate neurons in the murine cerebral cortex Cereb Cortex 2008. 19008461
17. Becker AJ Chen J Paus S Normann S Beck H Elger CE Wiestler OD Blümcke I Transcriptional profiling in human epilepsy: Expression array and single cell real-time qRT-PCR analysis reveal distinct cellular gene regulation Neuroreport 2002, 13:1327-33. 10.1097/ 00001756-200207190-00023 12151797
18. Schroeder A Mueller O Stocker S Salowsky R Leiber M Gassmann M Lightfoot S Menzel W Granzow M Ragg T The RIN: An RNA integrity number for assigning integrity values to RNA measurements BMC Mol Biol 2006, 7:3. 1413964 16448564 10.1186/1471-2199-7-3
19. Bulfone A Smiga S Shimamura K Peterson A Puelles L Rubenstein JLR T-Brain-1: A homolog of Brachyury whose expression defines molecularly distinct domains within the cerebral cortex Neuron 1995, 15:63-78. 10.1016/0896-6273(95)90065-9 7619531
20. Moon I Moturi S Goldrick M Isolate high quality total RNA from LCM samples suitable for microarray and qRT-PCR analysis Ambion Tech Notes 2005, 12(1): http://www.ambion.com/techlib/tn/121/15.html
21. Khodosevich K Inta D Seeburg PH Monyer H Gene expression analysis of in vivo fluorescent cells PLoS ONE 2007, 2:e1151. 2063466 17987128 10.1371/ journal.pone.0001151
22. Espina V Wulfkuhle JD Calvert VS VanMeter A Zhou W Coukos G Geho DH Petricoin EF III Liotta LA Laser-capture microdissection Nat Protoc 2006, 1:586-603. 10.1038/nprot.2006.85 17406286