Increase in furfural tolerance in ethanologenic Escherichia coli LY180 by plasmid-based expression of ThyA

Applied and Environmental Microbiology

Volumn 78, Issue 12, 2012, Pages 4346-4352

  Zheng, Huabao ^a   Wang, Xuan ^a   Yomano, Lorraine P ^a   Shanmugam, Keelnatham T ^a   Ingram, Lonnie O ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS SUBTILIS; CELLULAR RESPONSE; DE NOVO PATHWAY; DEOXYURIDINE; DNA REPAIR; E. COLI; GENOMIC LIBRARIES; MINERAL ACID; OXIDATIVE DAMAGE; SIDE PRODUCTS; SUBTILIS; TETRAHYDROFOLATES; THYMIDYLATE SYNTHASE; ZYMOMONAS MOBILIS;

ALDEHYDES; AMINO ACIDS; BIOCHEMISTRY; DNA; ESCHERICHIA COLI; GENE EXPRESSION;

FURFURAL;

ALCOHOL; FURFURAL; HEMICELLULOSE; POLYSACCHARIDE; RECOMBINANT PROTEIN; THYMIDYLATE SYNTHASE;

CELLULOSE; COLIFORM BACTERIUM; GENE EXPRESSION; GENOME; INHIBITOR; PLASMID; POLYMERIZATION; TOLERANCE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACILLUS SUBTILIS; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PLASMID; ZYMOMONAS;

BACILLUS SUBTILIS; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; ETHANOL; FURALDEHYDE; GENE EXPRESSION; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; PLASMIDS; POLYSACCHARIDES; RECOMBINANT PROTEINS; THYMIDYLATE SYNTHASE; ZYMOMONAS;

BACILLUS SUBTILIS; ESCHERICHIA COLI; ZYMOMONAS MOBILIS;

EID: 84864081563     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00356-12     Document Type: Article

References (37)

1. Ahmad SI, Kirk SH, Eisenstark A. 1998. Thymine metabolism and thymineless death in prokaryotes and eukaryotes. Annu. Rev. Microbiol. 52:591-625.
2. Allen SA, et al. 2010. Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae. Biotechnol. Biofuels 3:2.
3. Almeida JR, Bertilsson M, Gorwa-Grauslund MF, Gorsich S, Lidén G. 2009. Metabolic effects of furaldehydes and impacts on biotechnological processes. Appl. Microbiol. Biotechnol. 82:625-638.
4. Chandel AK, Singh OV. 2011. Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'biofuel.' Appl. Microbiol. Biotechnol. 89:1289-1303.
5. Changchien LM, et al. 2000. High-level expression of Escherichia coli and Bacillus subtilis thymidylate synthases. Protein Expr. Purif. 19:265-270.
6. Chundawat SP, et al. 2010. Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments. Bioresour. Technol. 101: 8429-8438.
7. Diaz De Villegas ME, et al. 1992. Conversion of furfural into furfuryl alcohol by Saccharomyces cerevisiae 354. Acta Biotechnol. 12:351-354.
8. Friedkin M. 1963. Assay of thymidylate synthetase activity. Methods Enzymol. 6:124-130.
9. Geddes CC, Nieves IU, Ingram LO. 2011. Advances in ethanol production. Curr. Opin. Biotechnol. 22:312-319.
10. Geddes CC, et al. 2010. Optimizing the saccharification of sugarcane bagasse using dilute phosphoric acid followed by fungal cellulases. Bioresour. Technol. 101:1851-1857.
11. Geddes CC, et al. 2010. Optimizing cellulase usage for improved mixing and rheological properties of acid-pretreated sugarcane bagasse. Bioresour. Technol. 101:9128-9136.
12. Gnansounou E, Dauriat A. 2010. Techno-economic analysis of lignocellulosic ethanol: a review. Bioresour. Technol. 101:4980-4991.
13. Haertlé T, Wohlrab F, Guschlbauer W. 1979. Thymidylate synthetase from Escherichia coli K12. Purification, and dependence of kinetic properties on sugar conformation and size of the 2= substituent. Eur. J. Biochem. 102:223-230.
14. Heer D, Heine D, Sauer U. 2009. Resistance of Saccharomyces cerevisiae to high furfural concentration is based on NADPH-dependent reduction by at least two oxireductases. Appl. Environ. Microbiol. 75:7631-7638.
15. Heer D, Sauer U. 2008. Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain. Microb. Biotechnol. 6:497-506.
16. Khan QA, Hadi SM. 1993. Effect of furfural on plasmid DNA. Biochem. Mol. Biol. Int. 29:1153-1160.
17. Liu ZL. 2011. Molecular mechanisms of yeast tolerance and in situ detoxification of lignocellulose hydrolysates. Appl. Microbiol. Biotechnol. 90: 809-825.
18. Liu ZL, Moon J, Andersh BJ, Slininger PJ, Weber S. 2008. Multiple gene-mediated NAD(P)H-dependent aldehyde reduction is a mechanism of in situ detoxification of furfural and 5-hydroxymethylfurfural by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 81:743-753.
19. Liu ZL, Slininger PJ, Gorsich SW. 2005. Enhanced biotransformation of furfural and hydroxymethylfurfural by newly developed ethanologenic yeast strains. Appl. Biochem. Biotechnol. 121-124:451-460.
20. Martinez A, et al. 2007. Low salt medium for lactate and ethanol production by recombinant Escherichia coli B. Biotechnol. Lett. 29:397-404.
21. Martinez A, Rodriguez ME, York SW, Preston JF, Ingram LO. 2000. Use of UV absorbance to monitor furans in dilute acid hydrolysates of biomass. Biotechnol. Prog. 16:637-641.
22. Martinez A, Rodriguez ME, York SW, Preston JF, Ingram LO. 2000. Effects of Ca(OH)2 treatments (" overliming") on the composition and toxicity of bagasse hemicellulose hydrolysates. Biotechnol. Bioeng. 69: 526-536.
23. Martinez A, et al. 2001. Detoxification of dilute acid hydrolysates of lignocellulose with lime. Biotechnol. Prog. 17:287-293.
24. Matthews RG. 1996. One-carbon metabolism, p 600-611. In Neidhardt FC, et al (ed), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed, vol 1. ASM Press, Washington, DC.
25. Miller EN, et al. 2009. Furfural inhibits growth by limiting sulfur assimilation in ethanologenic Escherichia coli strain LY180. Appl. Environ. Microbiol. 75:6132-6141.
26. Miller EN, et al. 2009. Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli. Appl. Environ. Microbiol. 75:4315-4323.
27. Mills TY, Sandoval NR, Gill RT. 2009. Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli. Biotechnol. Biofuels 2:26.
28. Neuhard J, Kelln RA. 1996. Biosynthesis and conversion of pyrimidines, p 580-599. In Neidhardt FC, et al (ed), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed, vol 1. ASM Press, Washington, DC.
29. Parawira W, Tekere M. 2011. Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review. Crit. Rev. Biotechnol. 31:20-31.
30. Rowe LA, Degtyareva N, Doetsch PW. 2008. DNA damage-induced reactive oxygen species (ROS) stress response in Saccharomyces cerevisiae. Free Radic. Biol. Med. 45:1167-1177.
31. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
32. Turner PC, et al. 2011. YqhC regulates transcription of the adjacent Escherichia coli genes yqhD and dkgA that are involved in furfural tolerance. J. Ind. Microbiol. Biotechnol. 38:431-439.
33. Wang X, et al. 2011. Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate. Appl. Environ. Microbiol. 77:5132-5140.
34. Wanner BL, Kodaira R, Neidhardt FC. 1977. Physiological regulation of a decontrolled lac operon. J. Bacteriol. 130:212-222.
35. Wyman CE, et al. 2009. Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies. Biotechnol. Prog. 25:333-339.
36. Zaldivar J, Martinez A, Ingram LO. 1999. Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli. Biotechnol. Bioeng. 65:24-33.
37. Zdzienicka M, Tudek B, Zielenska M, Szymczyk T. 1978. Mutagenic activity of furfural in Salmonella typhimurium TA100. Mutat. Res. 58:205-209.