Establishment of a microcarrier culture system with serial sub-cultivation for functionally active human endothelial cells

Journal of Biotechnology

Volumn 160, Issue 3-4, 2012, Pages 202-213

  Tashiro, Shinnya ^a   Tsumoto, Kouhei ^a   Sano, Emiko ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Endothelial cell growth; Endothelium functions; Human endothelial cells; Large scale culture; Microcarrier culture; Shear strength

Indexed keywords

ABNORMAL CHANGE; ARTIFICIAL SKIN; BIOLOGICAL FUNCTIONS; BIOLOGICAL PRODUCTS; BIOLOGICAL SUBSTANCE; CELL CONCENTRATIONS; CORONARY ARTERIES; CULTURE CONDITIONS; CULTURE SYSTEMS; DO CONTROL; ENDOTHELIN-1; EXTRACELLULAR MATRICES; FLOW EFFECTS; GROWTH CHARACTERISTIC; GROWTH FACTOR; HUMAN ENDOTHELIAL CELLS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; IN-VITRO; IN-VIVO; LARGE-SCALE PRODUCTION; LIFE SPAN; MICROCARRIER CULTURE; MICROCARRIER SYSTEMS; MICROCARRIERS; MONOLAYER CULTURE; PRIMARY CELLS; RESTENOSIS; SERUM CONCENTRATION; VON WILLEBRAND FACTOR;

ARTIFICIAL ORGANS; BLOOD VESSELS; DISEASES; ENDOTHELIAL CELLS; MEDICAL APPLICATIONS; MONOLAYERS; SHEAR STRENGTH;

CELL CULTURE;

CD31 ANTIGEN; ENDOTHELIN 1; PROSTACYCLIN; VASCULOTROPIN 165; VON WILLEBRAND FACTOR;

ARTICLE; CELL CULTURE; CELL FUNCTION; CELL GROWTH; CHROMOSOME; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DIPLOIDY; DOWN REGULATION; HUMAN; HUMAN CELL; LIFESPAN; MICROCARRIER CULTURE; MONOLAYER CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SHEAR STRENGTH; UMBILICAL VEIN ENDOTHELIAL CELL;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; ENDOTHELIAL CELLS; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FLOW CYTOMETRY; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES;

EID: 84864056776     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.02.022     Document Type: Article

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