The Dac-tag, an affinity tag based on penicillin-binding protein 5

Analytical Biochemistry

Volumn 428, Issue 1, 2012, Pages 64-72

  Lee, David Wei ^a   Peggie, Mark ^b   Deak, Maria ^b   Toth, Rachel ^b   Gage, Zoe Olivia ^b   Wood, Nicola ^b   Schilde, Christina ^b   Kurz, Thimo ^b   Knebel, Axel ^b,c  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

^c Sir James Black Centre   (United Kingdom)

Author keywords

Affinity tag; Ampicillin Sepharose; Penicillin binding protein 5; Protein purification

Indexed keywords

ESCHERICHIA COLI; GENES;

AFFINITY TAG; AMPICILLIN SEPHAROSE; BETA-LACTAMS; EUKARYOTIC EXPRESSION; EXPRESSION SYSTEM; FUSION PROTEINS; PENICILLIN- BINDING PROTEINS; PROTEIN PURIFICATION;

PROTEINS;

AMPICILLIN SEPHAROSE; DAC TAG; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PENICILLIN BINDING PROTEIN 5; PEPTIDE FRAGMENT; POLYSACCHARIDE; PROTEIN TAG; UNCLASSIFIED DRUG;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BINDING AFFINITY; CELL STRAIN HEK293; EMBRYO; ESCHERICHIA COLI; EXPRESSION VECTOR; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SYNTHESIS;

ESCHERICHIA COLI; EUKARYOTA;

EID: 84864037626     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.06.007     Document Type: Article

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