Loss of the retinoblastoma tumor suppressor protein in murine calvaria facilitates immortalization of osteoblast-adipocyte bipotent progenitor cells characterized by low expression of N-cadherin

Molecular and Cellular Biology

Volumn 32, Issue 13, 2012, Pages 2561-2569

  Gündüz, Volkan ^a,b   Kong, Elizabeth ^a   Bryan, Crystal D ^a   Hinds, Philip W ^a  

^a TUFTS UNIVERSITY   (United States)

^b NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; NERVE CELL ADHESION MOLECULE; RETINOBLASTOMA PROTEIN; TUMOR SUPPRESSOR PROTEIN;

ADIPOCYTE; ANIMAL CELL; ARTICLE; BIPOTENT PROGENITOR CELL; CALVARIA; CELL ADHESION; CELL IMMORTALIZATION; CELL ISOLATION; CELL MIGRATION; CELL POPULATION; CONTROLLED STUDY; EMBRYO; GENE INACTIVATION; MOUSE; NONHUMAN; OSTEOBLAST; OSTEOSARCOMA; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETINOBLASTOMA; STEM CELL; TUMOR SUPPRESSOR GENE;

3T3 CELLS; ADIPOCYTES; ADIPOGENESIS; ANIMALS; BASE SEQUENCE; CADHERINS; CELL MOVEMENT; CELLS, CULTURED; DNA PRIMERS; EMBRYONIC STEM CELLS; GENE EXPRESSION; GENES, RETINOBLASTOMA; MICE; MICE, KNOCKOUT; MULTIPOTENT STEM CELLS; OSTEOBLASTS; RETINOBLASTOMA PROTEIN; RNA, MESSENGER; SKULL;

MURINAE;

EID: 84864018515     PISSN: 02707306     EISSN: 10985549     Source Type: Journal    
DOI: 10.1128/MCB.06453-11     Document Type: Article

References (29)

1. Arima Y, et al. 2008. Rb depletion results in deregulation of E-cadherin and induction of cellular phenotypic changes that are characteristic of the epithelial-to-mesenchymal transition. Cancer Res. 68:5104 -5112.
2. Calo E, et al. 2010. Rb regulates fate choice and lineage commitment in vivo. Nature 466:1110 -1114.
3. Castro CH, et al. 2004. Targeted expression of a dominant-negative N-cadherin in vivo delays peak bone mass and increases adipogenesis. J. Cell Sci. 117:2853-2864.
4. Cheng SL, Shin CS, Towler DA, Civitelli R. 2000. A dominant negative cadherin inhibits osteoblast differentiation. J. Bone Miner. Res. 15:2362- 2370.
5. Ducy P, Schinke T, Karsenty G. 2000. The osteoblast: a sophisticated fibroblast under central surveillance. Science 289:1501-1504.
6. Fajas L, et al. 2002. The retinoblastoma-histone deacetylase 3 complex inhibits PPARgamma and adipocyte differentiation. Dev. Cell 3:903-910.
7. Ferrari SL, et al. 2000. A role for N-cadherin in the development of the differentiated osteoblastic phenotype. J. Bone Miner. Res. 15:198 -208.
8. Fink L, et al. 1998. Real-time quantitative RT-PCR after laser-assisted cell picking. Nat. Med. 4:1329 -1333.
9. Gutierrez GM, et al. 2008. Impaired bone development and increased mesenchymal progenitor cells in calvaria of RB1-/- mice. Proc. Natl. Acad. Sci. U. S. A. 105:18402-18407.
10. Hansen JB, te Riele H, Kristiansen K. 2004. Novel function of the retinoblastoma protein in fat: regulation of white versus brown adipocyte differentiation. Cell Cycle 3:774 -778.
11. Kashima T, et al. 1999. Anomalous cadherin expression in osteosarcoma. Possible relationships to metastasis and morphogenesis. Am. J. Pathol. 155:1549 -1555.
12. Kashima T, et al. 2003. Overexpression of cadherins suppresses pulmonary metastasis of osteosarcoma in vivo. Int. J. Cancer 104:147-154.
13. Lee JS, et al. 2006. HES1 cooperates with pRb to activate RUNX2-dependent transcription. J. Bone Miner. Res. 21:921-933.
14. Marie PJ. 2002. Role of N-cadherin in bone formation. J. Cell. Physiol. 190:297-305.
15. Morito S, et al. 2005. Insulin signaling in adipocytes differentiated from mouse stromal MC3T3-G2/PA6 cells. Biol. Pharm. Bull. 28:2040 -2045.
16. Peinado H, Portillo F, Cano A. 2004. Transcriptional regulation of cadherins during development and carcinogenesis. Int. J. Dev. Biol. 48: 365-375.
17. Ramirez ME. 1992. Measurement of subcutaneous adipose tissue using ultrasound images. Am. J. Phys. Anthropol. 89:347-357.
18. Rodriguez-Salas N, et al. 2001. Beta-catenin expression pattern in small cell lung cancer: correlation with clinical and evolutive features. Histol. Histopathol. 16:353-358.
19. Savage SA, Mirabello L. 2011. Using epidemiology and genomics to understand osteosarcoma etiology. Sarcoma 2011:548151.
20. Sosa-Garcia B, et al. 2010. A role for the retinoblastoma protein as a regulator of mouse osteoblast cell adhesion: implications for osteogenesis and osteosarcoma formation. PLoS One 5:e13954. doi:10.1371/journal.pone.0013954.
21. Stains JP, Civitelli R. 2005. Cell-cell interactions in regulating osteogenesis and osteoblast function. Birth Defects Res. C Embryo Today 75:72- 80.
22. Stains JP, Civitelli R. 2005. Cell-to-cell interactions in bone. Biochem. Biophys. Res. Commun. 328:721-727.
23. Tang N, Song WX, Luo J, Haydon RC, He TC. 2008. Osteosarcoma development and stem cell differentiation. Clin. Orthop. Relat. Res. 466: 2114-2130.
24. Thomas D, Kansara M. 2006. Epigenetic modifications in osteogenic differentiation and transformation. J. Cell Biochem. 98:757-769.
25. Thomas DM, et al. 2001. The retinoblastoma protein acts as a transcriptional coactivator required for osteogenic differentiation. Mol. Cell 8:303- 316.
26. Thomas DM, Yang HS, Alexander K, Hinds PW. 2003. Role of the retinoblastoma protein in differentiation and senescence. Cancer Biol. Ther. 2:124 -130.
27. Tiemann F, Hinds PW. 1998. Induction of DNA synthesis and apoptosis by regulated inactivation of a temperature-sensitive retinoblastoma protein. EMBO J. 17:1040 -1052.
28. Van Aken EH, et al. 2002. Structure and function of the N-cadherin/catenin complex in retinoblastoma. Investig. Ophthalmol. Vis. Sci. 43: 595-602.
29. Zou X, et al. 2002. Cdk4 disruption renders primary mouse cells resistant to oncogenic transformation, leading to Arf/p53-independent senescence. Genes Dev. 16:2923-2934.