Gene silencing of Toll-like receptor 2 inhibits proliferation of human liver cancer cells and secretion of inflammatory cytokines

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Huang, Yuzheng ^a   Cai, Bing ^b   Xu, Ming ^a   Qiu, Zhiqin ^c   Tao, Yonghui ^a   Zhang, Ying ^a   Wang, Jie ^a   Xu, Yongliang ^a   Zhou, Yonghua ^a   Yang, Jing ^a   Han, Xiaofeng ^c   Gao, Qi ^a  

^a BEIJING HOSPITAL   (China)

^b WUXI PEOPLE S HOSPITAL AFFILIATED TO NANJING MEDICAL UNIVERSITY   (China)

^c WUXI NO 2 PEOPLE S HOSPITAL   (China)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 6; INTERLEUKIN 8; MESSENGER RNA; SMALL INTERFERING RNA; TOLL LIKE RECEPTOR 10; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 3; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 5; TOLL LIKE RECEPTOR 6; TOLL LIKE RECEPTOR 7; TOLL LIKE RECEPTOR 8; TOLL LIKE RECEPTOR 9;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CELL PROLIFERATION; CONTROLLED STUDY; CYTOKINE RELEASE; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; INFLAMMATION; LIVER CELL CARCINOMA; MALE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; RNA INTERFERENCE; TUMOR VOLUME;

ANIMALS; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; CYTOKINES; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; HUMANS; INFLAMMATION; LIVER NEOPLASMS; MALE; MICE; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TOLL-LIKE RECEPTOR 2; TRANSFECTION;

FUNGI; MUS;

EID: 84864008620     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038890     Document Type: Article

References (27)

1. Muccioli M, Sprague L, Nandigam H, Pate M, Benencia F, (2012) Toll-like receptors as novel therapeutic targets for ovarian cancer. ISRN Oncol 2012: 642141.
2. Hassan F, Islam S, Tumurkhuu G, Naiki Y, Koide N, et al. (2006) Intracellular expression of toll-like receptor 4 in neuroblastoma cells and their unresponsiveness to lipopolysaccharide. BMC cancer 6: 281-288.
3. Ilvesaro JM, Merrell MA, Swain TM, Davidson J, Zayzafoon M, et al. (2007) Toll like receptor-9 agonists stimulate prostate cancer invasion in vitro. Prostate 67: 774-781.
4. Droemann D, Albrecht D, Gerdes J, Ulmer AJ, Branscheid D, et al. (2005) Human lung cancer cells express functionally active Toll-like receptor 9. Respir Res 6: 1.
5. Molteni M, Marabella D, Orlandi C, Rossetti C, (2006) Melanoma cell lines are responsive in vitro to lipopolysaccharide and express TLR-4. Cancer Lett 235: 75-83.
6. Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T, (2006) TLR3 can directly trigger apoptosis in human cancer cells. J Immunol 176: 4894-4901.
7. Huang B, Zhao J, Shen S, Li H, He KL, et al. (2007) Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res 67: 4346-4352.
8. Ren T, Wen ZK, Liu ZM, Liang YJ, Guo ZL, et al. (2007) Functional expression of TLR9 is associated to the metastatic potential of human lung cancer cell: functional active role of TLR9 on tumor metastasis. Cancer Biol Ther 6: 1704-1709.
9. Kim S, Takahashi H, Lin WW, Descargues P, Grivennikov S, et al. (2009) Carcinoma-produced factors activate myeloid cells through TLR2 to stimulate metastasis. Nature 457: 102-106.
10. Hua D, Liu MY, Cheng ZD, Qin XJ, Zhang HM, et al. (2009) Small interfering RNA-directed targeting of Toll-like receptor 4 inhibits human prostate cancer cell invasion, survival, and tumorigenicity. Mol Immunol 46: 2876-2884.
11. Thompson AJ, Colledge D, Rodgers S, Wilson R, Revill P, et al. (2009) Stimulation of the interleukin-1 receptor and Toll-like receptor 2 inhibits hepatitis B virus replication in hepatoma cell lines in vitro. Antivir Ther 14: 797-808.
12. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408.
13. Huang B, Zhao J, Unkeless JC, Feng ZH, Xiong H, (2008) TLR signaling by tumor and immune cells: a double-edged sword. Oncogene 27: 218-224.
14. Kawai T, Akira S, (2010) The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat Immunol 11: 373-384.
15. Huang Y, Yang G, Kurian D, Xu M, Dai Y, et al. (2011) Proteomic patterns as biomarkers for the early detection of schistosomiasis japonica in a rabbit model. Int J of Mass Spectrom 299: 191-195.
16. Yang H-Z, Cui B, Liu H-Z, Mi S, Yan J, et al. (2009) Blocking TLR2 Activity Attenuates Pulmonary Metastases of Tumor. PLoS ONE 4: e6520.
17. Xu N, Yao HP, Sun Z, Chen Z, (2008) Toll-like receptor 7 and 9 expression in peripheral blood mononuclear cells from patients with chronic hepatitis B and related hepatocellular carcinoma. Acta Pharmacol Sin 29: 239-244.
18. Orihara K, Nagata K, Hamasaki S, Oba R, Hirai H, et al. (2007) Time-course of Toll-like receptor 2 expression, as a predictor of recurrence in patients with bacterial infectious diseases. Clin Exp Immunol 148: 260-270.
19. Hise AG, Daehnel K, Gillette-Ferguson I, Cho E, McGarry HF, et al. (2007) Innate immune responses to endosymbiotic Wolbachia bacteria in Brugia malayi and Onchocerca volvulus are dependent on TLR2, TLR6, MyD88, and Mal, but not TLR4, TRIF, or TRAM. J Immunol 178: 1068-1076.
20. Kelly MG, Alvero AB, Chen R, Silasi DA, Abrahams VM, et al. (2006) TLR-4 signaling promotes tumor growth and paclitaxel chemoresistance in ovarian cancer. Cancer Res 66: 3859-3868.
21. Hritz I, Mandrekar P, Velayudham A, Catalano D, Dolganiuc A, et al. (2008) The critical role of toll-like receptor (TLR) 4 in alcoholic liver disease is independent of the common TLR adapter MyD88. Hepatology 48: 1224-1231.
22. Matzinger P, (2002) The danger model: a renewed sense of self. Science 296: 301-305.
23. Lien E, Sellati TJ, Yoshimura A, Flo TH, Rawadi G, et al. (1999) Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem 274: 33419-33425.
24. Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, et al. (2001) The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 410: 1099-1103.
25. Motyka B, Reynolds JD, (1991) Apoptosis is associated with the extensive B cell death in the sheep ileal Peyer's patch and the chicken bursa of Fabricius: a possible role in B cell selection. Eur J Immunol 21: 1951-1958.
26. Baccala R, Hoebe K, Kono DH, Beutler B, Theofilopoulos AN, (2007) TLR-dependent and TLR-independent pathways of type I interferon induction in systemic autoimmunity. Nat Med 13: 543-551.
27. Testro AG, Visvanathan K, (2009) Toll-like receptors and their role in gastrointestinal disease. J Gastroenterol Hepatol 24: 943-954.