Multicolour single molecule imaging on cells using a supercontinuum source

Biomedical Optics Express

Volumn 3, Issue 3, 2012, Pages 400-406

  Webb, Stephen E D ^a   Zanetti Domingues, Laura ^a   Coles, Benjamin C ^a   Rolfe, Daniel J ^a   Wareham, Richard J ^b   Martin Fernandez, Marisa L ^a  

^a Harwell Science and Innovation Campus   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; FLUORESCENCE; LASER EXCITATION; LIGHT SOURCES; PROTEINS;

EASE-OF-USE; EXCITATION SOURCES; LASER WAVELENGTH; LIVING CELL; MEMBRANE PROTEINS; SINGLE MOLECULE FLUORESCENCE; SINGLE WAVELENGTH; SINGLE-MOLECULE IMAGING; SUPERCONTINUUM LASER; SUPERCONTINUUM SOURCES; WHITE LIGHT SOURCES;

MOLECULES;

EID: 84863911148     PISSN: None     EISSN: 21567085     Source Type: Journal    
DOI: 10.1364/BOE.3.000400     Document Type: Article

References (17)

1. Y. Yarden and M. X. Sliwkowski, "Untangling the ErbB signalling network," Nat. Rev. Mol. Cell Biol. 2(2), 127-137 (2001).
2. P. R. Selvin and T. Ha, Single Molecule Techniques: a Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 2007).
3. Y. Sako, "Imaging single molecules in living cells for systems biology," Mol. Syst. Biol. 2, 56 (2006).
4. D. Axelrod, "Total internal reflection fluorescence microscopy in cell biology," Traffic 2(11), 764-774 (2001).
5. J. A. Hern, A. H. Baig, G. I. Mashanov, B. Birdsall, J. E. T. Corrie, S. Lazareno, J. E. Molloy, and N. J. M. Birdsall, "Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules," Proc. Natl. Acad. Sci. U.S.A. 107(6), 2693-2698 (2010).
6. K. Ritchie, X. Y. Shan, J. Kondo, K. Iwasawa, T. Fujiwara, and A. Kusumi, "Detection of non-Brownian diffusion in the cell membrane in single molecule tracking," Biophys. J. 88(3), 2266-2277 (2005).
7. P. D. Dunne, R. A. Fernandes, J. McColl, J. W. Yoon, J. R. James, S. J. Davis, and D. Klenerman, "DySCo: quantitating associations of membrane proteins using two-color single-molecule tracking," Biophys. J. 97(4), L5-L7 (2009).
8. J. F. Beausang, H. W. Schroeder III, P. C. Nelson, and Y. E. Goldman, "Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay," Biophys. J. 95(12), 5820-5831 (2008).
9. V. C. DeRocco, T. Anderson, J. Piehler, D. A. Erie, and K. Weninger, "Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes," Biotechniques 49(5), 807-816 (2010).
10. J. Vogelsang, T. Cordes, C. Forthmann, C. Steinhauer, and P. Tinnefeld, "Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy," Proc. Natl. Acad. Sci. U.S.A. 106(20), 8107-8112 (2009).
11. D. M. Owen, E. Auksorius, H. B. Manning, C. B. Talbot, P. A. A. de Beule, C. Dunsby, M. A. A. Neil, and P. M. W. French, "Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source," Opt. Lett. 32(23), 3408-3410 (2007).
12. D. Wildanger, E. Rittweger, L. Kastrup, and S. W. Hell, "STED microscopy with a supercontinuum laser source," Opt. Express 16(13), 9614-9621 (2008).
13. Y. Sako, S. Minoghchi, and T. Yanagida, "Single-molecule imaging of EGFR signalling on the surface of living cells," Nat. Cell Biol. 2(3), 168-172 (2000).
14. D. J. Rolfe, C. I. McLachlan, M. Hirsch, S. R. Needham, C. J. Tynan, S. E. D. Webb, M. L. Martin-Fernandez, and M. P. Hobson, "Automated multidimensional single molecule fluorescence microscopy feature detection and tracking," Eur. Biophys. J. 40(10), 1167-1186 (2011).
15. P. Blandin, S. Lévêque-Fort, S. Lécart, J. C. Cossec, M. C. Potier, Z. Lenkei, F. Druon, and P. Georges, "Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source," Appl. Opt. 48(3), 553-559 (2009).
16. B. Agnarsson, S. Ingthorsson, T. Gudjonsson, and K. Leosson, "Evanescent-wave fluorescence microscopy using symmetric planar waveguides," Opt. Express 17(7), 5075-5082 (2009).
17. J. P. Knemeyer, D. P. Herten, and M. Sauer, "Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy," Anal. Chem. 75(9), 2147-2153 (2003).