Optimized production of MLM triacylglycerols catalyzed by immobilized heterologous Rhizopus oryzae lipase

JAOCS, Journal of the American Oil Chemists' Society

Volumn 89, Issue 7, 2012, Pages 1287-1295

  Nunes, P A ^a,b   Pires Cabral, P ^a,b   Guillen M ^c   Valero, F ^c   Ferreira Dias, S ^a,b  

^a UNIVERSITY OF ALGARVE   (Portugal)

^b INSTITUTO SUPERIOR DE AGRONOMIA   (Portugal)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Acidolysis; Capric acid; Caprylic acid; Olive oil; Recombinant Rhizopus oryzae lipase; Response surface methodology; Structured lipids

Indexed keywords

ACIDOLYSIS; CAPRIC ACID; CAPRYLIC ACID; OLIVE OIL; RESPONSE SURFACE METHODOLOGY; RHIZOPUS ORYZAE LIPASE; STRUCTURED LIPIDS;

CATALYSIS; GLYCEROL; OPTIMIZATION; SATURATED FATTY ACIDS; SURFACE PROPERTIES; VEGETABLE OILS; YEAST;

EXPERIMENTS;

PICHIA PASTORIS; RHIZOPUS ORYZAE;

EID: 84863779322     PISSN: 0003021X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11746-012-2027-9     Document Type: Article

References (31)

1. Xu X (2000) Production of specific-structured triacylglycerols by lipase-catalyzed reactions: a review. Eur J Lipid Sci Technol 102:287-303
2. Smith RE, Finley JW, Leveille GA (1994) Overview of SALATRIM, a family of low-calorie fats. J Agric Food Chem 42(2):432-434
3. Osborn HT, Akoh CC (2002) Structured lipids-novel fat with medical, nutraceutical and food applications. Compr Rev Food Sci Saf 1(3):110-120
4. Iwasaki Y, Yamane T (2000) Enzymatic synthesis of structured lipids. J Mol Catal B: Enzym 10(1-3):129-140
5. Huang KH, Akoh CC (1996) Optimization and scale up of enzymatic synthesis of structured lipids using RSM. J Food Sci 6:137-141
6. Reena R, Udayasankar K, Samabaiah K, Lokesh BR (2001) Enzymatic acidolysis in hexane to produce n-3 or n-6 FA-enriched structured lipids from coconut oil: optimization of reactions by response surface methodology. J Am Oil Chem Soc 79:885-890
7. Carrin ME, Crapiste HG (2008) Enzymatic acidolysis of sunflower oil with a palmitic-stearic acid mixture. J Food Eng 84:243-249
8. Fomuso BL, Akoh CC (2002) Lipase-catalyzed acidolysis of olive oil and caprylic acid in a bench-scale packed bed bioreactor. Food Res Int 35:15-21
9. Sharma M, Rastogi NK, Lokesh BR (2009) Synthesis of structured lipid with balanced omega-3: omega-6 ratio by lipase-catalyzed acidolysis reaction: optimization of reaction using response surface methodology. Process Biochem 44:1284-1288
10. Zhou D, Xu X, Mu H, Hoy C-E, Adler-Nissen J (2001) Synthesis of structured triacylglycerols containing caproic acid by lipasecatalyzed acidolysis: optimization by response surface methodology. J Agric Food Chem 49:5771-5777
11. Kim BH, Akoh CC (2005) Modelling of lipase-catalyzed acidolysis of sesame oil and caprylic acid by response surface methodology: optimization of reaction conditions by considering both acyl incorporation and migration. J Agric Food Chem 53:8033-8037
12. Can A, Özçelik B (2005) Enrichment of hazelnut oil with longchain n-3 PUFA by lipase-catalyzed acidolysis: optimization by response surface methodology. J Am Oil Chem Soc 82:27-32
13. Bektas I, Sevil Y, Ustun G, Aksoy HA (2008) Production of reduced calorie structured lipid by acidolysis of tripalmitin with capric acid: optimisation by response surface methodology. J Sci Food Agric 88:1927-1931
14. Öztürk T, Ustun G, Aksoy HA (2010) Production of mediumchain triacylglycerols from corn oil: optimization by response surface methodology. Bioresour Technol 101:7456-7461
15. Nunes PA, Pires-Cabral P, Guillén M, Valero F, Ferreira-Dias S (2011) Production of MLM-type structured lipids catalyzed by immobilized heterologous Rhizopus oryzae lipase. J Am Oil Chem Soc 88:473-480
16. Nunes PA, Pires-Cabral P, Ferreira-Dias S (2011) Production of olive oil enriched with medium chain fatty acids catalysed by commercial immobilised lipases. Food Chem 127(3):993-998
17. Guillén M, Benaiges MD, Valero F (2011) Comparison of the biochemical properties of a recombinant lipase extract from Rhizopus oryzae expressed in Pichia pastoris with a native extract. Biochem Eng J 54:117-123
18. Covas MI (2007) Olive oil and the cardiovascular system. Pharmacol Res 55(3):175-186
19. Arnau C, Ramón R, Casas C, Valero F (2010) Optimization of the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system using mixed substrates on controlled fed-batch bioprocess. Enzyme Microb Technol 46:494-500
20. Cos O, Serrano A, Montesinos JL, Ferrer P, Cregg JM, Valero F (2005) Combined effect of methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia. Pastoris fed-batch cultures J Biotechnol 116(4):321-335
21. Resina D, Serrano A, Valero F, Ferrer P (2004) Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol 109:103-113
22. Bradford MM (1976) A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248-254
23. Gacula MC Jr, Singh J (1984) Response surface designs and analysis, in: Statistical Methods in Food and Consumer Research, Food Science and Technology. A Series of Monographs. Academic Press, Orlando, p 214
24. Haaland PD (1989) In: Owen DB (ed) Experimental design in biotechnology, statistics: textbooks and monographs. Marcel Dekker Inc, New York, p 258
25. Jennings BH, Akoh CC (1999) Enzymatic modification of triacylglycerols of high eicosapentaenoic and docosahexaenoic acids content to produce structured lipids. J Am Oil Chem Soc 76:1133-1137
26. Camacho Paez B, Robles Medina A, Camacho Rubio F, Estebán Cerdán L, Molina Grima E (2003) Kinetics of lipase-catalysed interesterification of triolein and caprylic acid to produce structured lipids. J Chem Technol Biotechnol 78:461-470
27. Jennings BH, Akoh CC (2000) Lipase-catalysed modification of rice bran oil to incorporate capric acid. J Agric Food Chem 48(9):4439-4443
28. Wu XY, Jääskeläinen S, Linkom Y-Y (1996) An investigation of crude lipases for hydrolysis, esterification, and transesterification. Enzyme Microb Technol 19:226-231
29. Sharma R, Chisti Y, Banerjee UC (2001) Production, purification, characterization, and applications of lipases. Biotechnol Adv 19(8):627-662
30. Haas MJ, Allen J, Berka TR (1991) Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar. Gene 109:107-113
31. Haas MJ, Bailey DG, Baker W, Berka TR, Cichowicz DJ, Derewenda ZS, Genuario RR, Joerger RD, Klein RR, Scott K, Woolf DJ (1999) Biochemical and molecular biological characterization of a lipase produced by the fungus Rhizopus delemar. Fett/Lipid 101:364-370