Rapid detection of bloodstream pathogens by real-time PCR in patients with sepsis

Wiener Klinische Wochenschrift

Volumn 124, Issue 7-8, 2012, Pages 266-270

  Grif, Katharina ^a   Fille, Manfred ^a   Würzner, Reinhard ^a   Weiss, Günter ^a   Lorenz, Ingo ^a   Gruber, Gottfried ^a   Eschertzhuber, Stephan ^b   Nachbaur, David ^a   Lass Flörl, Cornelia ^a   Orth, Dorothea ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b UNIVERSITY HOSPITAL INNSBRUCK   (Austria)

Author keywords

Blood culture; Multiplex RT PCR; Pathogen identification; Sepsis

Indexed keywords

AMIKACIN; AMPHOTERICIN B; CEFTAZIDIME; CEFTRIAXONE; CEFUROXIME; CIPROFLOXACIN; COLISTIN; COTRIMOXAZOLE; ERYTHROMYCIN; FLUCONAZOLE; FOSFOMYCIN; IMIPENEM; LEVOFLOXACIN; LINEZOLID; MEROPENEM; METRONIDAZOLE; PIPERACILLIN PLUS TAZOBACTAM; TOBRAMYCIN; VANCOMYCIN; VORICONAZOLE;

ADULT; ANTIBIOTIC THERAPY; ARTICLE; ASSAY; AUSTRIA; BLOOD CULTURE; BLOOD SAMPLING; BLOODSTREAM INFECTION; COHORT ANALYSIS; CONTROLLED STUDY; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; NONHUMAN; OBSERVATIONAL STUDY; PREDICTIVE VALUE; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEPSIS; SEPTIFAST ASSAY; TERTIARY HEALTH CARE;

BACTEREMIA; BACTERIA; BLOOD-BORNE PATHOGENS; FEMALE; HUMANS; MALE; MICROBIOLOGICAL TECHNIQUES; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84863725337     PISSN: 00435325     EISSN: 16137671     Source Type: Journal    
DOI: 10.1007/s00508-012-0159-4     Document Type: Article

References (11)

1. Beekmann SE, Diekema DJ, Chapin KC, Doern GV. Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol. 2003;41(7):3119-25. (Pubitemid 36842309)
2. Struelens MJ. Detection of microbial DNAemia: does it matter for sepsis management? Intensive Care Med. 2010;36(2):193-5.
3. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM. New developments in the diagnosis of bloodstream infections. Lancet Infect Dis. 2004;4(12):751-60. (Pubitemid 39574347)
4. Casalta JP, Gouriet F, Roux V, Thuny F, Habib G, Raoult D. Evaluation of the LightCycler SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis. Eur J Clin Microbiol Infect Dis. 2009;28(6):569-73.
5. Lehmann LE, Hunfeld KP, Emrich T, et al. A multiplex realtime PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immun. 2008;197(3):313-24.
6. Varani S, Stanzani M, Paolucci M, et al. Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J Infect. 2009;58(5):346-51.
7. Lucignano B, Ranno S, Liesenfeld O, et al. Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J Clin Microbiol. 2011;49(6):2252-8.
8. Mencacci A, Leli C, Cardaccia A, Montagna P, et al. Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood. J Med Microbiol. 2011;60:1774-8.
9. Maubon D, Hamidfar-Roy R, Courby S, et al. Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis. J Infect. 2010;61(4):335-42.
10. Wallet F, Nseir S, Baumann L, et al. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect. 2010;16(6):774-9.
11. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH. The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest. 2000;118(1):146-55. (Pubitemid 30485502)