A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress

Cell Stress and Chaperones

Volumn 17, Issue 2, 2012, Pages 275-279

  Van Schadewijk, Annemarie ^a   Van'T Wout, Emily F A ^a   Stolk, Jan ^a   Hiemstra, Pieter S ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

BiP; CHOP; ER stress; Primary bronchial epithelial cells; Real time RT PCR; Spliced XBP1

Indexed keywords

THAPSIGARGIN; TUNICAMYCIN; X BOX BINDING PROTEIN 1;

AMINO ACID SEQUENCE; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM STRESS; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; VALIDATION PROCESS;

ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BIOLOGICAL MARKERS; CELLS, CULTURED; DNA-BINDING PROTEINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOPLASMIC RETICULUM; ENZYME INHIBITORS; EPITHELIAL CELLS; HUMANS; LUNG NEOPLASMS; MOLECULAR SEQUENCE DATA; OXIDATIVE STRESS; POLYMERASE CHAIN REACTION; RNA SPLICING; RNA, MESSENGER; STRESS, PHYSIOLOGICAL; THAPSIGARGIN; TRANSCRIPTION FACTORS; TUNICAMYCIN;

EID: 84863586566     PISSN: 13558145     EISSN: 14661268     Source Type: Journal    
DOI: 10.1007/s12192-011-0306-2     Document Type: Article

References (9)

1. Hirota M, Kitagaki M, Itagaki H, Aiba S (2006) Quantitative measurement of spliced XBP1 mRNA as an indicator of endoplasmic reticulum stress. J Toxicol Sci 31:149-156 (Pubitemid 43894270)
2. Ron D, Walter P (2007) Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol 8:519-529 (Pubitemid 46985379)
3. Rutkowski DT, Kaufman RJ (2004) A trip to the ER: coping with stress. Trends Cell Biol 14:20-28 (Pubitemid 38076857)
4. Samali A, Fitzgerald U, Deegan S, Gupta S (2010) Methods for monitoring endoplasmic reticulum stress and the unfolded protein response. Int J Cell Biol 2010:830307
5. Schroder M, Kaufman RJ (2005) The mammalian unfolded protein response. Annu Rev Biochem 74:739-789 (Pubitemid 40995523)
6. van Wetering S, van der Linden AC, van Sterkenburg MA, Rabe KF, Schalkwijk J, Hiemstra PS (2000) Regulation of secretory leukocyte proteinase inhibitor (SLPI) production by human bronchial epithelial cells: increase of cell-associated SLPI by neutrophil elastase. J Investig Med 48:359-366
7. Vandesompele J, de Preter K, Pattyn F, Poppe B, van Roy N, de Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:RESEARCH0034
8. Yoshida H, Matsui T, Yamamoto A, Okada T, Mori K (2001) XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor Cell 107:881-891 (Pubitemid 34084979)
9. Zhao H, Yang J, Shan L, Jorgensen ED (2011) Measuring the impact ofcigarette smoke on the UPR. Methods Enzymol 489:147-164