High-resolution structures of Neotermes koshunensis β-glucosidase mutants provide insights into the catalytic mechanism and the synthesis of glucoconjugates

Acta Crystallographica Section D: Biological Crystallography

Volumn 68, Issue 7, 2012, Pages 829-838

  Jeng, Wen Yih ^a,b,c   Wang, Nai Chen ^a,b   Lin, Cheng Tse ^a,b   Chang, Wei Jung ^a,b   Liu, Chia I ^a,b   Wang, Andrew H J ^a,b  

^a INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^b ACADEMIA SINICA   (Taiwan)

^c NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

glucosidases; glycosyl hydrolase family 1; Neotermes koshunensis; NkBgl; oligosaccharide synthesis; transglycosylation

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; BETA GLUCOSIDASE; GLUCOSE; GLYCOCONJUGATE; OLIGOSACCHARIDE;

ANIMAL; ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; GLYCOSYLATION; ISOPTERA; METABOLISM; MUTATION; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

ANIMALS; BETA-GLUCOSIDASE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; GLUCOSE; GLYCOCONJUGATES; GLYCOSYLATION; HEPES; ISOPTERA; MODELS, MOLECULAR; MUTATION; OLIGOSACCHARIDES; PROTEIN CONFORMATION;

NEOTERMES KOSHUNENSIS;

EID: 84863461798     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S0907444912013224     Document Type: Article

References (28)

1. Bradford, M. M. (1976). Anal. Biochem. 72, 248-254.
2. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.
3. Crout, D. H. & Vic, G. (1998). Curr. Opin. Chem. Biol. 2, 98-111.
4. Dwek, R. A. (1996). Chem. Rev. 96, 683-720.
5. Emsley, P. & Cowtan, K. (2004). Acta Cryst. D60, 2126-2132.
6. Gopalan, V., Glew, R. H., Libell, D. P. & DePetro, J. J. (1989). J. Biol. Chem. 264, 15418-15422.
7. Hanson, S., Best, M., Bryan, M. C. & Wong, C.-H. (2004). Trends Biochem. Sci. 29, 656-663.
8. Jeng, W.-Y., Wang, N.-C., Lin, M.-H., Lin, C.-T., Liaw, Y.-C., Chang, W.-J., Liu, C.-I., Liang, P.-H. &Wang, A. H.-J. (2011). J. Struct. Biol. 173, 46-56.
9. Kim, Y.-W., Zhang, R., Chen, H. & Withers, S. G. (2010). Chem. Commun. 46, 8725-8727.
10. Laskowski, R. A., Chistyakov, V. V. & Thornton, J. M. (2005). Nucleic Acids Res. 33, D266-D268.
11. Lovell, S. C., Davis, I. W., Arendall, W. B. III, De Bakker, P. I., Word, J. M., Prisant, M. G., Richardson, J. S. & Richardson, D. C. (2003). Proteins, 50, 437-450.
12. Mackenzie, L. F.,Wang, Q.,Warren, R. A. J. &Withers, S. G. (1998). J. Am. Chem. Soc. 120, 5583-5584.
13. Miller, G. L. (1959). Anal. Chem. 31, 426-428.
14. Murshudov, G. N., Skubák, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Long, F. & Vagin, A. A. (2011). Acta Cryst. D67, 355-367.
15. Otwinowski, Z. & Minor,W. (1997). Methods Enzymol. 276, 307-326.
16. Perugino, G., Trincone, A., Rossi, M. & Moracci, M. (2004). Trends Biotechnol. 22, 31-37.
17. Shaikh, F. A. &Withers, S. G. (2008). Biochem. Cell Biol. 86, 169-177.
18. Takahashi, E. & Wraight, C. A. (1991). FEBS Lett. 283, 140-144.
19. Tokuda, G., Saito, H. & Watanabe, H. (2002). Insect Biochem. Mol. Biol. 32, 1681-1689.
20. Tran, V., Hoffmann, L., Rabiller, C., Tellier, C. & Dion, M. (2010). Protein Eng. Des. Sel. 23, 43-49.
21. Umezurike, G. M. (1988). Biochem. J. 254, 73-76.
22. Vaguine, A. A., Richelle, J. & Wodak, S. J. (1999). Acta Cryst. D55, 191-205.
23. Varki, A. (1993). Glycobiology, 3, 97-130.
24. Wang, L.-X. & Huang, W. (2009). Curr. Opin. Chem. Biol. 13, 592-600.
25. Watt, D. K., Ono, H. & Hayashi, K. (1998). Biochim. Biophys. Acta, 1385, 78-88.
26. Winn, M. D. et al. (2011). Acta Cryst. D67, 235-242.
27. Winn, M. D., Murshudov, G. N. & Papiz, M. Z. (2003). Methods Enzymol. 374, 300-321.
28. Zopf, D. & Roth, S. (1996). Lancet, 347, 1017-1021.