Rational design of a GH1 β-glycosidase to prevent self-condensation during the transglycosylation reaction

Protein Engineering, Design and Selection

Volumn 23, Issue 1, 2010, Pages 43-49

  Tran, Vinh ^a   Hoffmann, Lionel ^a   Rabiller, Claude ^a   Tellier, Charles ^a   Dion, Michel ^a  

^a UNIVERSITÉ DE NANTES   (France)

Author keywords

glycosidase; Enzyme substrate modeling; N acetyl glucosamine; Oligosaccharide; Transglycosylation

Indexed keywords

DIRECTED EVOLUTION; DOUBLE MUTANTS; GALACTOPYRANOSIDE; GLUCOSAMINES; GLYCOSIDASES; GLYCOSIDIC BOND; RATIONAL DESIGN; SELF-CONDENSATION; SUBSITES; SUBSTRATE MODELING; TRANS GLYCOSYLATION ACTIVITY; TRANSGLYCOSYLATION; TRANSGLYCOSYLATION REACTION; UNDESIRABLE EFFECTS;

CONDENSATION; ENZYMES; HYDROGEN BONDS; METHOD OF MOMENTS; OLIGOSACCHARIDES; SYNTHESIS (CHEMICAL);

CONDENSATION REACTIONS;

2 NITROPHENYL BETA DEXTRO GALACTOPYRANOSIDE; BETA GLYCOSIDASE; GALACTOSE; GLYCOSIDASE; PYRANOSIDE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE MUTATION; GLYCOSYLATION; HYDROGEN BOND; MOLECULAR MODEL; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; WILD TYPE;

BINDING SITES; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; GLYCOSIDE HYDROLASES; GLYCOSYLATION; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN ENGINEERING; THERMUS THERMOPHILUS; TRANSFERASES;

EID: 73849138550     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp068     Document Type: Article

References (23)

1. Beauharnois, M.E., Lindquist, K.C., Marathe, D., Vanderslice, P., Xia, J., Matta, K.L. and Neelamegham, S. (2005) Biochemistry, 44, 9507-9519.
2. Brassart, D., Woltz, A., Golliard, M. and Neeser, J.R. (1991) Infect. Immun., 59, 1605-1613.
3. Chiffoleau-Giraud, V., Spangenberg, P., Dion, M. and Rabiller, C. (1999) European J. Org. Chem., 757-763.
4. Dinur, U. and Hagler, A.T. (1991) In Boyd, D.B. (ed.), Review of Computational Chemistry with Discover-Insight II Packages of Accelrys. Vol.2. San Diego, CA, USA.
5. Dion, M., Fourage, F. and Hallet, J.N. (1999) Glycoconj. J., 16, 27-37.
6. Drone, J., Dion, M., Tellier, C. and Rabiller, C. (2007) Protein Eng. Des. Sel., 20, 7-14.
7. Feng, H.Y., Drone, J., Hoffmann, L., Tran, V., Tellier, C., Rabiller, C. and Dion, M. (2005) J. Biol. Chem., 280, 37088-37097.
8. Hancock, S.M., Vaughan, M.D. and Withers, S.G. (2006) Curr. Opin. Chem. Biol., 10, 509-519.
9. Imberty, A. and Varrot, A. (2008) Curr. Opin. Struct. Biol., 18, 567-576.
10. Kone, F.M., Le Bechec, M., Sine, J.P., Dion, M. and Tellier, C. (2009) Protein Eng. Des. Sel., 22, 37-44.
11. Krivan, H.C., Roberts, D.D. and Ginsburg, V. (1988) Proc. Natl Acad. Sci. USA, 85, 6157-6161.
12. Ly, H.D. and Withers, S.G. (1999) Annu. Rev. Biochem., 68, 487-522.
13. Mackenzie, L.F., Wang, Q., Warren, R.A.J. and Withers, S.G. (1998) J. Am. Chem. Soc., 120, 5583-5584.
14. Mayer, C., Zechel, D.L., Reid, S.P., Warren, R.A. and Withers, S.G. (2000) FEBS Lett., 466, 40-44.
15. Osanjo, G., Dion, M., Drone, J., Solleux, C., Tran, V., Rabiller, C. and Tellier, C. (2007) Biochemistry, 46, 1022-1033.
16. Prade, H., Mackenzie, L.F. and Withers, S.G. (1998) Carbohydr. Res., 305, 371-381.
17. Ruiz-Palacios, G.M., Cervantes, L.E., Ramos, P., Chavez-Munguia, B. and Newburg, D.S. (2003) J. Biol. Chem., 278, 14112-14120.
18. Schengrund, C.L. (2003) Biochem. Pharmacol., 65, 699-707.
19. Shirato, H., et al. (2008) J. Virol., 82, 10756-10767.
20. Simon, P.M., Goode, P.L., Mobasseri, A. and Zopf, D. (1997) Infect. Immun., 65, 750-757.
21. Spangenberg, P., Andre, C., Dion, M., Rabiller, C. and Mattes, R. (2000) Carbohydr. Res., 329, 65-73.
22. Usui, T., Kubota, S. and Ohi, H. (1993) Carbohydr. Res., 244, 315-323.
23. Varki, A. (1997) J. Clin. Invest., 100, S31-S35.