Recovery of functionally active recombinant human phospholipid scramblase 1 from inclusion bodies using N-lauroyl sarcosine

Journal of Industrial Microbiology and Biotechnology

Volumn 39, Issue 7, 2012, Pages 1041-1048

  Francis, Vincent Gerard ^a   Majeed, Mohammed Abdul ^a   Gummadi, Sathyanarayana N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

Author keywords

EF hand motif; Human phospholipid scramblase 1; Inclusion body; N lauroyl sarcosine; Overexpression; Tryptophan fluorescence

Indexed keywords

CALCIUM ION; HUMAN PHOSPHOLIPID SCRAMBLASE 1; N LAUROYL SARCOSINE; NITRILOTRIACETATE NICKEL; SARCOSINE; SCRAMBLASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ALPHA HELIX; ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; LIPID BILAYER; NONHUMAN; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; SOLUBILIZATION;

CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; ESCHERICHIA COLI; FLUORESCENCE; HUMANS; INCLUSION BODIES; MEMBRANE PROTEINS; PHOSPHOLIPID TRANSFER PROTEINS; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SARCOSINE; TRYPTOPHAN;

ESCHERICHIA COLI;

EID: 84863428483     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-012-1105-1     Document Type: Article

References (26)

1. Basse F, Stout JG, Sims P, Wiedmer T (1996) Isolation of an erythrocyte membrane protein that mediates Ca2?-dependent transbilayer movement of phospholipid. J Biol Chem 271:17205-17210 (Pubitemid 26244274)
2. Bateman A, Finn RD, Sims PJ, Wiedmer T, Biegert A, Soding J (2008) Phospholipid scramblase and Tubby like protein belong to a new super family of membrane tethered transcription factors. Bioinformatics 25:159-162
3. Callis PR (1997) 1La and 1Lb transitions of tryptophan: Applications of theory and experimental observations to fluorescence of proteins. Methods Enzymol 278:113-150 (Pubitemid 27229918)
4. Chang GHF, Barbaro NM, Pieper RO (2000) Phosphatidyl serine dependant phagocytosis of apoptotic glioma cells by normal microglia, astrocytes, and glioma cells. Neuro Oncol 2:174-183
5. Douette P, Navet R, Bouillenne F, Brans A, Sluse-Goffart C, Matagne A, Sluse EF (2004) Secondary structure characterization by far-UV CD of highly purified uncoupling protein1 expressed in yeast. Biochem J 380:139-145 (Pubitemid 38725738)
6. Drew D, Lerch M, Kunji E, Slotboom DJ, De Gier JW (2006) Optimization of membrane protein over expression and purification using GFP fusions. Nat Methods 3:303-313
7. Fischer B, Summer I, Goodenough P (1974) Isolation, renaturation and formation of disulfide bonds of eukaryotic proteins expressed in Escherichia coli as inclusion bodies. Biotechnol Bioeng 41:3-13
8. Gummadi SN, Menon AK (2002) Transbilayer movement of dipalmitoylphosphatidylcholine in proteoliposomesreconstituted from detergent extracts of endoplasmic reticulum: Kinetics of transbilayer transport mediated by a single flippase and identification of protein fractions enriched in flippase activity. J Biol Chem 227:25337-25343 (Pubitemid 34951844)
9. Hamada H, Arakawa T, Shiraki K (2009) Effect of additives on protein aggregation. Curr Pharm Biotechnol 10:400-407
10. Jesevar S, Gaberc-Porekar V, Fonda I, Podobnik B, Grdadolnik J, Menart V (2005) Production of non classical inclusion bodies from which correctly folded proteins can be extracted. Biotechnol Prog 21:632-639 (Pubitemid 40466443)
11. Korepanova A, Moore JD, Nguyen HB, Hua Y, Cross TA, Gao F (2007) Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein. Protein Expr Purif 53:24-30 (Pubitemid 46283455)
12. Kurucz I, Titus JA, Jost CR, Seagal DM (1995) Correct disulfide pairing and efficient refolding of detergent-solubilized single chain Fv proteins from bacterial inclusion bodies. Mol Immunol 32:1443-1452 (Pubitemid 26064909)
13. Niegowski D, Hedren M, Nordlund P, Eshaghi S (2006) A simple strategy towards membrane protein purification and crystallization. Int J Biol Macromol 39:83-87 (Pubitemid 44066542)
14. Peternal S, Grdadolink J, Gaberc-Porekar V, Komel R (2008) Engineering inclusion bodies for non denaturing extraction of functional proteins. Microb Cell Fact 7:34
15. Sahu SK, Gummadi SN, Aradhyam GK (2007) Phospholipid scramblases: An overview. Arch Biochem Biophys 462:103-114 (Pubitemid 46755145)
16. Sahu SK, Gummadi SN, Aradhyam GK (2008) Over-expression of recombinant human phospholipid scramblase 1 in E. coli and its purification from inclusion bodies. Biotech Lett 30:2131-2137
17. Sahu SK, Archita R, Gummadi SN (2009) GroES and GroEL are essential chaperones for refolding of recombinant human phospholipid scramblase 1 in E.Coli. Biotechnol Lett 31:1745-1752
18. Stout JG, Zhou Q, Wiedmer T, Sims PJ (1998) Change in conformation of plasma membrane phospholipid scramblase induced by occupancy of its Ca2? binding site. Biochemistry 37:14860-14866 (Pubitemid 28487594)
19. Tokatlidis K, Dhurjati P, Millet J, Beguin P, Aubert JP (1991) High activity of inclusion bodies formed in E. coli overproducing Clostridium thermocellum endoglucanase. FEBS Lett 282:205-208
20. Vanhove M, Lejeune A, Pain RH (1998) β-lactamases as models for protein folding studies. Cell Mol Life Sci 54:372-377 (Pubitemid 28192745)
21. Vivian TJ, Calis RP (2000) Mechanism of tryptophan fluorescence shift in proteins. Biophys J 80:2093-2109
22. Wiedmer T, Zhao J, Nanjundan M, Sims PJ (2003) Palmitoylation of phospholipid scramblase 1 controls its distribution between nucleus and plasma membrane. Biochemistry 42:1227-1233 (Pubitemid 36184063)
23. Williamson P, Bevers ME, Smeets FE, Comfurius P, Schlegel AR, Zwaal AF (1995) Continous analysis of the mechanism of activated transbilayer lipid movement in platelets. Biochemistry 34:10448-10455
24. Worrall DM, Goss NH (1989) The formation of biologically active beta-galactosidase inclusion bodies in E. coli. Aust J Biotechnol 3:28-32
25. Zhou Q, Sims PJ, Wiedmer T (1998) Identity of a conserved motif in phospholipid scramblase that is required for Ca2?-accelerated transbilayer movement of membrane phospholipids. Biochemistry 37:2356-2360 (Pubitemid 28119309)
26. Zhou Q, Zhao J, Stout JG, Luhm RA, Wiedmer T, Sims PJ (1997) Molecular cloning of human plasma membrane phospholipid scramblase. J Biol Chem 272:18240-18244 (Pubitemid 27306412)