Over-expression of recombinant human phospholipid scramblase 1 in E. coli and its purification from inclusion bodies

Biotechnology Letters

Volumn 30, Issue 12, 2008, Pages 2131-2137

  Sahu, Santosh Kumar ^a   Gopala Krishna, A ^a   Gummadi, Sathyanarayana N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

Author keywords

EF hand motif; Inclusion body; Phospholipids; Protein refolding; Scramblase 1; Tryptophan fluorescence

Indexed keywords

AMINO ACIDS; CALCIUM; CELL CULTURE; CELL DEATH; CELL MEMBRANES; COAGULATION; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; LIGHT EMISSION; LIPIDS; LUMINESCENCE; METABOLISM; PURIFICATION; UREA; WATER POLLUTION;

EF-HAND MOTIF; INCLUSION BODY; PROTEIN REFOLDING; SCRAMBLASE 1; TRYPTOPHAN FLUORESCENCE;

PHOSPHOLIPIDS;

1 PALMITOYL 2 (6 (N (7 NITROBENZ) 2 OXA 1,3 DIAZOL 4 YL)AMINOCAPROYL)PHOSPHATIDYLCHOLINE; 1,2 (PALMITOYL NBD AMINOCAPROYL)PHOSPHATIDYLETHANOLAMINE; 1,2-(PALMITOYL-NBD-AMINOCAPROYL)PHOSPHATIDYLETHANOLAMINE; 1-PALMITOYL-2-(6-(N-(7-NITROBENZ)-2-OXA-1,3-DIAZOL-4-YL)AMINOCAPROYL)PHOSPHATIDYLCHOLINE; 4 CHLORO 7 NITROBENZOFURAZAN; CALCIUM; CARBOCYANINE; DITHIONITE; DRUG DERIVATIVE; NICKEL NITRILOTRIACETIC ACID; NITRILOTRIACETIC ACID; ORGANOMETALLIC COMPOUND; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHOLIPID TRANSFER PROTEIN; PLSCR1 PROTEIN, HUMAN; PROTEOLIPID; PROTEOLIPOSOMES; RECOMBINANT PROTEIN; STAINS-ALL; UREA;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN FOLDING; SOLUBILITY; SPECTROFLUOROMETRY; STAINS ALL;

4-CHLORO-7-NITROBENZOFURAZAN; CALCIUM; CARBOCYANINES; CHROMATOGRAPHY, AFFINITY; DITHIONITE; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; INCLUSION BODIES; NITRILOTRIACETIC ACID; ORGANOMETALLIC COMPOUNDS; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; PHOSPHOLIPID TRANSFER PROTEINS; PROTEIN FOLDING; PROTEOLIPIDS; RECOMBINANT PROTEINS; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; UREA;

ESCHERICHIA COLI;

EID: 54849405314     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9797-z     Document Type: Article

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