Evaluation of the cytotoxicity of cigarette smoke condensate by a cellular impedance biosensor

Food and Chemical Toxicology

Volumn 50, Issue 3-4, 2012, Pages 612-618

  Chen, Huan ^a   Cui, Lin ^b   Jiang, Xing Yi ^a   Pang, Yong Qiang ^a   Tang, Gang Ling ^a   Hou, Hong Wei ^a   Jiang, Jian Hui ^c   Hu, Qing Yuan ^a  

^a ZHENGZHOU TOBACCO RESEARCH INSTITUTE OF CNTC   (China)

^b THE FIRST AFFILIATED HOSPITAL OF HENAN UNIVERSITY OF TRADITIONAL CHINESE MEDICINE   (China)

^c HUNAN UNIVERSITY   (China)

Author keywords

Cellular impedance biosensor; Chinese hamster ovary cells; Cigarette smoke condensates; In vitro cytotoxicity assay; Real time kinetic measurement

Indexed keywords

CIGARETTE SMOKE CONDENSATE;

ARTICLE; BIOSENSOR; CELL ASSAY; CELL COUNT; CELL DENSITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; IC 50; INTERMETHOD COMPARISON; PROCESS OPTIMIZATION; QUALITY CONTROL; QUANTITATIVE ANALYSIS; TOXICITY TESTING;

ANIMALS; BIOSENSING TECHNIQUES; CELL SURVIVAL; CHO CELLS; CRICETINAE; CULTURE MEDIA; DIMETHYL SULFOXIDE; INHIBITORY CONCENTRATION 50; SMOKE; TOBACCO;

CRICETULUS GRISEUS;

EID: 84863280339     PISSN: 02786915     EISSN: 18736351     Source Type: Journal    
DOI: 10.1016/j.fct.2011.11.040     Document Type: Article

References (31)

1. Andreoli C., Gigante D., Nunziata A. A review of in vitro methods to assess the biological activity of tobacco smoke with the aim of reducing the toxicity of smoke. Toxicol. In vitro 2003, 17:587-594.
2. Atienza J.M., Yu N., Kirstein S.L., Xi B., Wang X., Xu X., Abassi Y.A. Dynamic and label-free cell-based assays using the real-time cell electronic sensing system. Assay Drug Dev. Technol. 2006, 4:597-607.
3. Baker R.R., Massey E.D., Smith G. An overview of the effects of tobacco ingredients on smoke chemistry and toxicity. Food Chem. Toxicol. 2004, 42S:S53-S83.
4. Bombick D.W., Doolittle D.J. The role of chemical structure and cell type in the cytotoxicity of low molecular weight aldehydes and pyridines. In Vitro Toxicol. 1995, 8:349-356.
5. Bombick D.W., Ayres P.H., Doolittle D.J. Cytotoxicity assessment of whole smoke and vapor phase of mainstream and sidestream cigarette smoke from three Kentucky reference cigarettes. Toxicol. Method 1997, 7:177-190.
6. Borgerding M., Klus H. Analysis of complex mixtures - Cigarette smoke. Exp. Toxicol. Pathol. 2005, 57S:43-73.
7. Boyd J.M., Huang L., Xie L., Moe B., Gabos S., Li X.F. A cell-microelectronic sensing technique for profiling cytotoxicity of chemicals. Anal. Chim. Acta 2008, 615:80-87.
8. Carmines E.L., Lemus R., Gaworski C.L. Toxicologic evaluation of licorice extract as a cigarette ingredient. Food Chem. Toxicol. 2005, 43:1303-1322.
9. Ceriotti L., Ponti J., Broggi F., Kob A., Drechsler S., Thedinga E., Colpo P., Sabbioni E., Ehret R., Rossi F. Real-time assessment of cytotoxicity by impedance measurement on a 96-well plate. Sensor. Actuat. B 2007, 123:769-778.
10. Counts M.E., Hsu F.S., Laffoon S.W., Dwyer R.W., Cox R.H. Mainstream smoke constituent yields and predicting relationships from a worldwide market sample of cigarette brands: ISO smoking conditions. Regul. Toxicol. Pharmacol. 2004, 39:111-134.
11. Fotakis G., Timbrell J.A. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol. Lett. 2006, 160:171-177.
12. Foy J.W.D., Bombick B.R., Bombick D.W., Doolittle D.J., Mosberg A.T., Swauger J.E. A comparison of in vitro toxicities of cigarette smoke condensate from Eclipse cigarettes and four commercially available ultra low-" tar" cigarettes. Food Chem. Toxicol. 2004, 42:237-243.
13. Glamann J., Hansen A.J. Dynamic detection of natural killer cell-mediated cytotoxicity and cell adhesion by electrical impedance measurements. Assay Drug Dev. Technol. 2006, 4:555-563.
14. Guo M., Chen J., Yun X., Chen K., Nie L., Yao S. Monitoring of cell growth and assessment of cytotoxicity using electrochemical impedance spectroscopy. Biochim. Biophys. Acta 2006, 1760:432-439.
15. Heck J.D. A review and assessment of menthol employed as a cigarette flavoring ingredient. Food Chem. Toxicol. 2010, 48S:S1-S38.
16. International Agency for research on cancer. Tobacco smoke and involuntary smoking. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. 83rd ed. Lyon (France): IARC; 2004.
17. International Organization for Standardization: International Standard ISO 3402, 1999. Tobacco and Tobacco Products - Atmosphere for Conditioning and Testing, 3rd Edition.
18. Lou J., Chu G., Zhou G., Jiang J., Huang F., Xu J., Zheng S., Chen Z., Jiang W., Lu Y., Li X., He J. Assessing cytogenotoxicity of cigarette smoke condensates using three in vitro assays. Mutat. Res. 2009, 677:21-26.
19. Lou J., Chu G., Zhou G., Jiang J., Huang F., Xu J., Zheng S., Jiang W., Lu Y., Li X., Chen Z., He J. Comparison between two kinds of cigarette smoke condensates (CSCs) of the cytogenotoxicity and protein expression in a human B-cell lymphoblastoid cell line using CCK-8 assay, comet assay and protein microarray. Mutat. Res. 2010, 697:55-59.
20. Martino A., Flamma F., Bassi A., Nunziata A., Andreoli C. Evaluation of the cytotoxicity of cigarette smoke condensate by using different solvents. Ig. Sanita. Pubbl. 2006, 62:241-254.
21. McAdam K.G., Gregg E.O., Liu C., Dittrich D.J., Duke M.G., Proctor C.J. The use of a novel tobacco-substitute sheet and smoke dilution to reduce toxicant yields in cigarette smoke. Food Chem. Toxicol. 2011, 49:1684-1696.
22. Opp D., Wafula B., Lim J., Huang E., Lo J.C., Lo C.M. Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity. Biosens. Bioelectron. 2009, 24:2625-2629.
23. Putnam K.P., Bombick D.W., Doolittle D.J. Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicol. In Vitro 2002, 16:599-607.
24. Qiu Y., Liao R., Zhang X. Real-time monitoring primary cardiomyocyte adhesion based on electrochemical impedance spectroscopy and electrical cell-substrate impedance sensing. Anal. Chem. 2008, 80:990-996.
25. Roemer E., Ottmueller T.H., Zenzen V., Wittke S., Radtke F., Blanco I., Carchman R.A. Cytotoxicity, mutagenicity, and tumorigenicity of mainstream smoke from three reference cigarettes machine-smoked to the same yields of total particulate matter per cigarette. Food Chem. Toxicol. 2009, 47:1810-1818.
26. Smith C.J., Hansch C. The relative toxicity of compounds in mainstream cigarette smoke condensate. Food Chem. Toxicol. 2000, 38:637-646.
27. Tewes F.J., Meisgen T.J., Veltel D.J., Roemer E., Patskan G. Toxicological evaluation of an electrically heated cigarette. Part 3: genotoxicity and cytotoxicity of mainstream smoke. J. Appl. Toxicol. 2003, 23:341-348.
28. Xia M., Huang R., Witt K.L., Southall N., Fostel J., Cho M.H., Jadhav A., Smith C.S., Inglese J., Portier C.J., Tice R.R., Austin C.P. Compound cytotoxicity profiling using quantitative high-throughput screening. Environ. Health Perspect. 2008, 116:284-291.
29. Xiao C., Luong J.H. Assessment of cytotoxicity by emerging impedance spectroscopy. Toxicol. Appl. Pharmacol. 2005, 206:102-112.
30. Xing J.Z., Zhu L., Jackson J.A., Gabos S., Sun X.J., Wang X.B., Xu X. Dynamic monitoring of cytotoxicity on microelectronic sensors. Chem. Res. Toxicol. 2005, 18:154-161.
31. Xing J.Z., Zhu L., Gabos S., Xie L. Microelectronic cell sensor assay for detection of cytotoxicity and prediction of acute toxicity. Toxicol. In Vitro 2006, 20:995-1004.