Targeted gene disruption in cryptococcus neoformans using double-joint pcr with split dominant selectable markers

Methods in Molecular Biology

Volumn 845, Issue , 2012, Pages 67-84

  Kim, Min Su ^a   Kim, Seo Young ^a   Jung, Kwang Woo ^a   Bahn, Yong Sun ^a  

^a Yonsei University   (South Korea)

Author keywords

Biolistic transformation; Cryptococcus neoformans; Double joint PCR; Fungal pathogen; Gene disruption; Neomycin G418; Nourseothricin acetyltransferase; Overlap PCR; Split dominant selectable markers

Indexed keywords

ARTICLE; CRYPTOCOCCUS NEOFORMANS; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; METHODOLOGY; POLYMERASE CHAIN REACTION;

CRYPTOCOCCUS NEOFORMANS; GENE DELETION; GENETIC MARKERS; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION;

FILOBASIDIELLA NEOFORMANS;

EID: 84863253814     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-539-8_5     Document Type: Chapter

References (9)

1. Robert C. Davidson1, Jill R. Blankenship1, et al. (2002) A PCR-based strategy to generate integrative targeting alleles with large regions of homology. Microbiology 148 (Pt 8): 2607–15.
2. D L Toffaletti, T H Rude, S A Johnston, et al. (1993) Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. J. Bacteriol 175(5):1405–1411.
3. Robert C. Davidson, M. Cristina Cruz, Rey A. L. Sia, et al. (2000) Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. Fungal Genet. Biol 29(1):38–48.
4. McDade HC, Cox GM. (2010) A new dominant selectable marker for use in Cryptococcus neoformans. Med. Mycol 39(1):151–154.
5. Nelson RT, Pryor BA, Lodge JK. (2003) Sequence length required for homologous recombination in Cryptococcus neoformans. Fungal Genet. Biol 38(1):1–9.
6. Kim MS, Kim SY, Yoon JK, et al. (2009) An efficient gene disruption method in Cryptococcus neoformans by double-joint PCR with NAT-split markers. Biochem. Biophys. Res. Commun 390(3):983–988.
7. Perfect JR, Ketabchi N, Cox GM, Ingram CW, et al. (1993) Karyotyping of Cryptococcus neoformans as an epidemiological tool. J. Clin. Microbiol 31(12):3305–9.
8. Nielsen K, Cox GM, Wang P, et al. (2003) Sexual cycle of Cryptococcus neoformans var. grubii and virulence of 650 congenic a and isolates. Infect Immun 71(9):4831–41.
9. Moore, T. D., Edman, J. C. (1993) The alpha-mating type locus of Cryptococcus neoformans contains a peptide pheromone gene. Mol. Cell. Biol 13(3):1962–70.