Rapid, specific detection of alphaviruses from tissue cultures using a replicon-defective reporter gene assay

PLoS ONE

Volumn 7, Issue 3, 2012, Pages

  Li, Jiangjiao ^a   Zhu, Wuyang ^a   Wang, Huanqin ^a   Li, Jiandong ^b   Zhang, Quanfu ^b   He, Ying ^a   Li, Jia ^a   Fu, Juanjuan ^a   Li, Dexin ^b   Liang, Guodong ^a  

^a CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

^b State Key Laboratory for Infectious Disease Prevention and Control (SKLID)   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GAUSSIA LUCIFERASE; NONSTRUCTURAL PROTEIN 4; UNCLASSIFIED DRUG; VIRUS VECTOR; GREEN FLUORESCENT PROTEIN; VIRUS PROTEIN;

ALPHA VIRUS; ARTICLE; CELL STRAIN BHK; CONTROLLED STUDY; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; GENETIC TRANSFECTION; JAPANESE ENCEPHALITIS VIRUS; LIMIT OF DETECTION; NONHUMAN; NSP4 GENE; NUCLEOTIDE SEQUENCE; PLASMID; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REPLICON; REPLICON DEFECTIVE REPORTER GENE ASSAY; REPORTER GENE; RNA VIRUS; SENSITIVITY AND SPECIFICITY; SINDBIS VIRUS; TAHYNA BUNYAVIRUS; TISSUE CULTURE; VIRUS DETECTION; VIRUS GENE; VIRUS GENOME; ALPHAVIRUS INFECTION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; VIRUS CULTURE;

ALPHAVIRUS; JAPANESE ENCEPHALITIS VIRUS; RNA VIRUSES; SINDBIS VIRUS; TAHYNA VIRUS;

ALPHAVIRUS; ALPHAVIRUS INFECTIONS; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LIMIT OF DETECTION; SENSITIVITY AND SPECIFICITY; SINDBIS VIRUS; VIRAL NONSTRUCTURAL PROTEINS; VIRAL PLAQUE ASSAY;

EID: 84863232915     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0033007     Document Type: Article

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