Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells

Sensors (Switzerland)

Volumn 12, Issue 6, 2012, Pages 6967-6977

  Jen, Chun Ping ^a   Amstislavskaya, Tamara G ^b   Liu, Ya Hui ^a   Hsiao, Ju Hsiu ^a   Chen, Yu Hung ^c  

^a NATIONAL CHUNG CHENG UNIVERSITY   (Taiwan)

^b INSTITUTE OF CYTOLOGY AND GENETICS   (Russian Federation)

^c NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

Electric lysis; Electroosmotic driven; Microfluidics; Microwell; Single cell

Indexed keywords

EID: 84863193766     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s120606967     Document Type: Article

References (29)

1. Price, A.K.; Culbertson, C.T. Chemical analysis of single mammalian cells with microfluidics. Anal. Chem. 2007, 79, 2614-2621.
2. Yoshimoto, K.; Ichinoa, M.; Nagasaki, Y. Inverted pattern formation of cell microarrays on poly(ethylene glycol) (PEG) gel patterned surface and construction of hepatocyte spheroids on unmodified PEG gel microdomains. Lab. Chip. 2009, 9, 1286-1289.
3. Folch, A.; Toner, M. Microenginggering of cellular interactions. Annu. Rev. Biomed. Eng. 2000, 2, 227-256.
4. Park, T.H.; Shuler, M.L. Integration of cell culture and microfabrication technology. Biotechnol. Prog. 2003, 19, 243-253.
5. Falconnet, D.; Csucs, G.; Grandin, H.M.; Textor, M. Surface engineering approaches to micropattern surfaces for cell-based assays. Biomaterials 2006, 27, 3044-3063.
6. Lim, J.Y.; Donahue, H.J. Cell sensing and response to micro-and nanostructured surfaces produced by chemical and topographic patterning. Tissue Eng. 2007, 13, 1879-1891.
7. Voldman, J.; Gray, M.L.; Toner, M.; Schmidt, M.A. A microfabrication-based dynamic array cytometer. Anal. Chem. 2002, 74, 3984-3990.
8. Ashkin, A. Optical trapping and manipulation of neutral particles using lasers. Proc. Natl. Acad. Sci. USA 1997, 94, 4853-4860.
9. Klauke, N.; Smith, G.L.; Cooper, J.M. Stimulation of single isolated adult ventricular myocytes within a low volume using a planar microelectrode array. Biophys. J. 2003, 85, 1766-1774.
10. Park, J.Y.; Morgan, M.; Sachs, A.N.; Samorezov, J.; Teller, R.; Shen, Y.; Pienta, K.J.; Takayama, S. Single cell trapping in larger microwells capable of supporting cell spreading and proliferation. Microfluid. Nanofluid. 2010, 8, 263-268.
11. Gromov, P.; Celis, J.E.; Gromova, I.; Rank, F.; Timmermans-Wielenga, V.; Moreira, J.M. A single lysis solution for the analysis of tissue samples by different proteomic technologies. Mol. Oncol. 2008, 2, 368-379.
12. Rettig, J.R.; Folch, A. Large-scale single-cell trapping and imaging using microwell arrays. Anal. Chem. 2005, 77, 5628-5634.
13. Brown, R.B.; Audet, J. Current techniques for single-cell lysis. J. R. Soc. Interface 2008, 5, S131-S138.
14. Rau, K.R.; Guerra, A.; Vogel, A.; Venugopalan, V. Investigation of laser-induced cell lysis using time-resolved imaging. Appl. Phys. Lett. 2004, 84, 2940-2942.
15. Di Carlo, D.; Jeong, K.H.; Lee, L.P. Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation. Lab. Chip. 2003, 3, 287-291.
16. Irimia, D.; Tompkins, R.G.; Toner, M. Single-cell chemical lysis in picoliter-scale closed volumes using a microfabricated device. Anal. Chem. 2004, 76, 6137-6143.
17. McClain, M.A.; Culbertson, C.T.; Jacobson, S.C.; Allbritton, N.L.; Sims, C.E.; Ramsey, J.M. Microfluidic devices for the high-throughput chemical analysis of cells. Anal. Chem. 2003, 75, 5646-5655.
18. He, M.; Edgar, J.S.; Jeffries, G.D.; Lorenz, R.M.; Shelby, J.P.; Chiu, D.T. Selective encapsulation of single cells and subcellular organelles into picoliter-and femtoliter-volume droplets. Anal. Chem. 2005, 77, 1539-1544.
19. Phillips, K.S.; Lai, H.H.; Johnson, E.; Sims, C.E.; Allbritton, N.L. Continuous analysis of dye-loaded, single cells on a microfluidic chip. Lab. Chip. 2011, 11, 1333-1341.
20. Huang, B.; Wu, H.; Bhaya, D.; Grossman, A.; Granier, S.; Kobilka, B.K.; Zare, R.N. Counting low-copy number proteins in a single cell. Science 2007, 315, 81-84.
21. Marc, P.J.; Sims, C.E.; Allbritton, N.L. Coaxial flow system for chemical cytometry. Anal. Chem. 2007, 79, 9054-9059.
22. Sasuga, Y.; Iwasawa, T.; Terada, K.; Oe, Y.; Sorimachi, H.; Ohara, O.; Harada, Y. Single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scale microwells. Anal. Chem. 2008, 80, 9141-9149.
23. Wang, H.-Y.; Lu, C. Microfluidic chemical cytometry based on modulation of local field strength. Chem. Commun. 2006, 33, 3528-3530.
24. Wang, H.-Y.; Lu, C. Microfluidic electroporation for delivery of small molecules and genes into cells using a common DC power supply. Biotechnol. Bioeng. 2008, 100, 579-586.
25. Lee, D.W.; Cho, Y.H. A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture. Sens. Actuators B 2007, 124, 84-89.
26. Mernier, G.; Piacentini, N.; Braschler, T.; Demierre, N.; Renaud, P. Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting. Lab. Chip. 2010, 10, 2077-2082.
27. Shafiee, H.; Caldwell, J.L.; Sano, M.B.; Davalos, R.V. Contactless dielectrophoresis: A new technique for cell manipulation. Biomed. Microd. 2009, 11, 997-1006.
28. van den Brink, F.T.G.; Gool, E.; Frimat, J.P.; Bomer, J.; van den Berg, A.; Le Gac, S. Parallel single-cell analysis microfluidic platform. Electrophoresis 2011, 32, 3094-3100.
29. Chung, K.; Rivet, C.A.; Kemp, M.L.; Lu, H. Imaging single-cell signaling dynamics with a deterministic high-density single-cell trap array. Anal. Chem. 2011, 83, 7044-7052.