Expression and purification of bioactive soluble murine stem cell factor from recombinant Escherichia coli using thioredoxin as fusion partner

Journal of Biotechnology

Volumn 152, Issue 1-2, 2011, Pages 1-8

  Bals, Carola ^a   Schambach, Axel ^b   Meyer, Johann ^b   Scheper, Thomas ^a   Rinas, Ursula ^a,c  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Escherichia coli; Fusion protein; Membrane adsorber technology; Soluble protein expression; Stem cell factor; Thioredoxin

Indexed keywords

FUSION PROTEIN; MEMBRANE ADSORBER TECHNOLOGY; SOLUBLE PROTEIN EXPRESSION; STEM CELL FACTOR; THIOREDOXINS;

ADSORPTION; BIOACTIVITY; CELL CULTURE; DIALYSIS; DIESEL ENGINES; ESCHERICHIA COLI; MEMBRANE TECHNOLOGY; PROTEINS; PURIFICATION;

STEM CELLS;

GAG PROTEIN; RECOMBINANT SOLUBLE MURINE STEM CELL FACTOR; RECOMBINANT STEM CELL FACTOR; THIOREDOXIN; THIOREDOXIN MURINE STEM CELL FACTOR FUSION PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CELL INCLUSION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE SEQUENCE; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; SOLUTION AND SOLUBILITY;

ANIMALS; CELL LINE; CELL PROLIFERATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; MICE; POLYMERASE CHAIN REACTION; STEM CELL FACTOR; THIOREDOXINS;

ESCHERICHIA COLI; MURINAE; TOBACCO ETCH VIRUS;

EID: 79952042906     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.01.012     Document Type: Article

References (47)

1. Alexander B., Browse D., Reading S., Benjamin I. A simple and accurate mathematical method for calculation of the EC50. J. Pharmacol. Toxicol. Methods 1999, 41:55-58.
2. Arakawa T., Yphantis D.A., Lary J.W., Narhi L.O., Lu H.S., Prestrelski S.J., Clogston C.L., Zsebo K.M., Mendiaz E.A., Wypych J., et al. Glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure. J. Biol. Chem. 1991, 266:18942-18948.
3. Avanzi G.C., Lista P., Giovinazzo B., Miniero R., Saglio G., Benetton G., Coda R., Cattoretti G., Pegorado L. Selective growth response to IL-3 of a human leukemic cell line with megakaryoblastic features. Br. J. Haematol. 1988, 69:359-366.
4. Ball E.H. Quantitation of proteins by elution of Coomassie brilliant blue R from stained bands after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 1986, 155:23-27.
5. Bazan J.F. Genetic and structural homology of stem cell factor and macrophage colony-stimulating factor. Cell 1991, 65:9-10.
6. Bernstein I.D., Andrews R.G., Zsebo K.M. Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+ line-cells, and the generation of colony-forming cell progeny from CD34+ line-cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor. Blood 1991, 77:2316-2321.
7. Bodine D.M., Seidel N.E., Gale M.S., Nienhuis A.W., Orlic D. Efficient retrovirus transduction of mouse pluripotent hematopoietic stem cells mobilized into the peripheral blood by treatment with granulocyte colony-stimulating factor and stem cell factor. Blood 1994, 84:1482-1491.
8. Brugger W., Heimfeld S., Berenson R.J., Mertelsmann R., Kanz L. Reconstitution of hematopoiesis after high-dose chemotherapy by autologous progenitor cells generated ex vivo. N. Engl. J. Med. 1995, 333:283-287.
9. Domen J., Weissman I.L. Hematopoietic stem cells need two signals to prevent apoptosis; BCL-2 can provide one of these Kitl/c-Kit signaling the other. J. Exp. Med. 2000, 192:1707-1718.
10. Erickson-Miller C.L., Ji H., Murphy M.J. Megakaryocytopoiesis and platelet production: does stem cell factor play a role?. Stem Cells 1993, 11:163-169.
11. Esposito D., Chatterjee D.K. Enhancement of soluble protein expression through the use of fusion tags. Curr. Opin. Biotechnol. 2006, 17:353-358.
12. Flanagan J.G., Leder P. The kit ligand: a cell surface molecule altered in steel mutant fibroblasts. Cell 1990, 63:185-194.
13. Fleming W.H., Alpern E.J., Uchida N., Ikuta K., Weissman I.L. Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo. Proc. Natl. Acad. Sci. U.S.A. 1993, 90:3760-3764.
14. Grage-Griebenow E., Zawatzky R., Kahlert H., Brade L., Flad H., Ernst M. Identification of a novel dendritic cell-like subset of CD64(+)/CD16(+) blood monocytes. Eur. J. Immunol. 2001, 31:48-56.
15. Hendrie P.C., Miyazawa K., Yang Y.C., Langefeld C.D., Broxmeyer H.E. Mast cell growth factor (c-kit ligand) enhances cytokine stimulation of proliferation of the human factor-dependent cell line, MO7e. Exp. Hematol. 1991, 19:1031-1037.
16. Huang E., Nocka K., Beier D., Chu T., Buck J., Lahm H., Wellner D., Leder P., Besmer P. The hematopoietic growth factor KL is encoded by the Sl-locus and is the ligand of the c-kit receptor, the gene-product of the W-locus. Cell 1990, 63:225-233.
17. Hubbard S.R., Till J.H. Protein tyrosine kinase structure and function. Annu. Rev. Biochem. 2000, 69:373-398.
18. Jones M.D., Narhi L.O., Chang W.C., Lu H.S. Refolding and oxidation of recombinant human stem cell factor produced in Escherichia coli. J. Biol. Chem. 1996, 271:11301-11308.
19. Kobayashi M., Laver J.H., Kato T., Miyazaki H., Ogawa M. Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3. Blood 1996, 88:429-436.
20. Kökpinar O., Harkensee D., Kasper C., Scheper T., Zeidler R., Reif O.W., Ulber R. Innovative modular membrane adsorber system for high-throughput downstream screening for protein purification. Biotechnol. Prog. 2006, 22:1215-1219.
21. Langley K.E., Mendiaz E.A., Liu N., Narhi L.O., Zeni L., Parseghian C.M., Clogston C.L., Leslie I., Pope J.A., Lu H.S., et al. Properties of variant forms of human stem cell factor recombinantly expressed in Escherichia coli. Arch. Biochem. Biophys. 1994, 311:55-61.
22. Langley K.E., Wypych J., Mendiaz E.A., Clogston C.L., Parker V.P., Farrar D.H., Brothers M.O., Satygal V.N., Leslie I., Birkett N.C., et al. Purification and characterization of soluble forms of human and rat stem cell factor recombinantly expressed by Escherichia coli and by Chinese hamster ovary cells. Arch. Biochem. Biophys. 1992, 295:21-28.
23. LaVallie E.R., DiBlasio E.A., Kovacic S., Grant K.L., Schendel P.F., McCoy J.M. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 1993, 11:187-193.
24. Leary A.G., Zeng H.Q., Clark S.C., Ogawa M. Growth factor requirements for survival in G0 and entry into the cell cycle of primitive human hemopoietic progenitors. Proc. Natl. Acad. Sci. U.S.A. 1992, 89:4013-4017.
25. Levesque J.P., Leavesley D.I., Niutta S., Vadas M., Simmons P.J. Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins. J. Exp. Med. 1995, 181:1805-1815.
26. Lili W., Chaozhan W., Xindu G. Expression, renaturation and simultaneous purification of recombinant human stem cell factor in Escherichia coli. Biotechnol. Lett. 2006, 28:993-997.
27. Lu H.S., Clogston C.L., Wypych J., Fausset P.R., Lauren S., Mendiaz E.A., Zsebo K.M., Langley K.E. Amino acid sequence and post-translational modification of stem cell factor isolated from buffalo rat liver cell-conditioned medium. J. Biol. Chem. 1991, 266:8102-8107.
28. Lyman S.D., Jacobsen S.E. c-Kit ligand and Flt3 ligand: stem/progenitor cell factors with overlapping yet distinct activities. Blood 1998, 91:1101-1134.
29. Martin F.H., Suggs S.V., Langley K.E., Lu H.S., Ting J., Okino K.H., Morris C.F., McNiece I.K., Jacobsen F.W., Mendiaz E.A., et al. Primary structure and functional expression of rat and human stem cell factor DNAs. Cell 1990, 63:203-211.
30. Potala S., Verma R.S. A novel fusion protein diphtheria toxin-stem cell factor (DT-SCF)-purification and characterization. Appl. Biochem. Biotechnol. 2010, 162:1258-1269.
31. Rennick D., Hunte B., Holland G., Thompson-Snipes L. Cofactors are essential for stem cell factor-dependent growth and maturation of mast cell progenitors: comparative effects of interleukin-3 (IL-3), IL-4, IL-10, and fibroblasts. Blood 1995, 85:57-65.
32. Schein C.H. Production of soluble recombinant proteins in bacteria. Bio/Technology 1989, 7:1141-1147.
33. Sette C., Dolci S., Geremia R., Rossi P. The role of stem cell factor and of alternative c-kit gene products in the establishment, maintenance and function of germ cells. Int. J. Dev. Biol. 2000, 44:599-608.
34. Smith M.A., Pallister C.J., Smith J.G. Stem cell factor: biology and relevance to clinical practice. Acta Haematol. 2001, 105:143-150.
35. Stewart E.J., Aslund F., Beckwith J. Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins. EMBO J. 1998, 17:5543-5550.
36. Su L., Chen S., Yang K., Liu C., Liang Z. Cloning and expression of human stem cell factor fused with thrombopoietin mimetic peptide in Escherichia coli. Biotechnol. Lett. 2006, 28:857-862.
37. Suck K., Walter J., Menzel F., Tappe A., Kasper C., Naumann C., Zeidler R., Scheper T. Fast and efficient protein purification using membrane adsorber systems. J. Biotechnol. 2006, 121:361-367.
38. Takashi Y., Kanei-Ishii C., Maekawa T., Fujimoto J., Yamamoto T., Ishii S. Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin. J. Biol. Chem. 1995, 270:25328-25331.
39. Tsai M., Takeishi T., Thompson H., Langley K.E., Zsebo K.M., Metcalfe D.D., Geissler E.N., Galli S.J. Induction of mast cell proliferation, maturation, and heparin synthesis by the rat c-kit ligand, stem cell factor. Proc. Natl. Acad. Sci. U.S.A. 1991, 88:6382-6386.
40. Wang C., Liu J., Wang L., Geng X. Solubilization and refolding with simultaneous purification of recombinant human stem cell factor. Appl. Biochem. Biotechnol. 2008, 144:181-189.
41. Wang L.L., Geng X.D., Han H. Cloning, expression, renaturation and purification of soluble hSCF. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2004, 20:402-405.
42. Williams D.E., Eisenman J., Baird A., Rauch C., Van Ness K., March C.J., Park L.S., Martin U., Mochizuki D.Y., Boswell H.S., et al. Identification of a ligand for the c-kit proto-oncogene. Cell 1990, 63:167-174.
43. Witte O.N. Steel locus defines new multipotent growth factor. Cell 1990, 63:5-6.
44. Yarden Y., Kuang W.J., Yang-Feng T., Coussens L., Munemitsu S., Dull T.J., Chen E., Schlessinger J., Francke U., Ullrich A. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 1987, 6:3341-3351.
45. Zang Y., Zhang X., Jiang X., Li H., Zhu J., Zhang C., Peng W., Qin J. Expression, refolding, and characterization of recombinant thrombopoietin/stem cell factor fusion protein in Escherichia coli. Appl. Microbiol. Biotechnol. 2007, 74:836-842.
46. Zhang Z., Zhang R., Joachimiak A., Schlessinger J., Kong X.P. Crystal structure of human stem cell factor: implication for stem cell factor receptor dimerization and activation. Proc. Natl. Acad. Sci. U.S.A. 2000, 97:7732-7737.
47. Zsebo K.M., Wypych J., McNiece I.K., Lu H.S., Smith K.A., Karkare S.B., Sachdev R.K., Yuschenkoff V.N., Birkett N.C., Williams L.R., et al. Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium. Cell 1990, 63:195-201.