Cell-free protein synthesis as a promising expression system for recombinant proteins

Methods in Molecular Biology

Volumn 824, Issue , 2012, Pages 565-578

  Ge, Xumeng ^a   Xu, Jianfeng ^a  

^a ARKANSAS STATE UNIVERSITY   (United States)

Author keywords

Cell free protein synthesis; In vitro transcription; In vitro translation; Renilla luciferase; Wheat germ extract

Indexed keywords

PLANT EXTRACT; RECOMBINANT PROTEIN; RENILLA LUCIFERIN 2 MONOOXYGENASE;

ARTICLE; BIOTECHNOLOGY; CELL FREE SYSTEM; CHEMISTRY; GENE VECTOR; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; METHODOLOGY; PHYSIOLOGY; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRENATAL DEVELOPMENT; PROTEIN SYNTHESIS; WESTERN BLOTTING; WHEAT;

BIOTECHNOLOGY; BLOTTING, WESTERN; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; LUCIFERASES, RENILLA; PLANT EXTRACTS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; SEEDS; TRANSCRIPTION, GENETIC; TRITICUM;

RENILLA LUCIFERASE; TRITICUM AESTIVUM;

EID: 84862969056     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-433-9_30     Document Type: Article

References (17)

1. Katzen, F., Chang, G., and Kudlicki, W. (2005) The past, present and future of cell-free protein synthesis, Trends Biotechnol. 23, 150-156. (Pubitemid 40269848)
2. He, M. Y. (2008) Cell-free protein synthesis: applications in proteomics and biotechnology, N. Biotechnol. 25, 126-132.
3. Sawasaki, T., Ogasawara, T., Morishita, R., et al., (2002) A cell-free protein synthesis system for high-throughput proteomics, Proc. Natl. Acad. Sci. USA 99, 14652-14657. (Pubitemid 35334539)
4. Jackson, A. M., Boutell, J., Cooley, N., et al., (2004) Cell-free protein synthesis for proteomics, Brief Funct. Genomic. Proteomic. 2, 308-319.
5. Klammt, C., Schwarz, D., Lohr, F., et al., (2006) Cell-free expression as an emerging technique for the large scale production of integral membrane protein, FEBS J. 273, 4141-4153. (Pubitemid 44326495)
6. Xu, Z. N., Chen, H. Q., Yin, X. F., et al., (2005) High-level expression of soluble human beta-defensin-2 fused with green fl uorescent protein in Escherichia coli cell-free system, Appl. Biochem. Biotech. 127, 53-62.
7. Vinarov, D. A., Lytle, B. L., Peterson, F. C., et al., (2004) Cell-free protein production and labeling protocol for NMR-based structural proteomics, Nat. Methods 1, 149-153.
8. Bundy, B. C., and Swartz, J. R. (2010) Site-Specifi c Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein- Protein Click Conjugation, Bioconjug. Chem. 21, 255-263.
9. Chattopadhaya, S., Tan, L. P., and Yao, S. Q. (2006) Strategies for site-specifi c protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays, Nat. Protoc. 1, 2386-2398.
10. Park, N., Kahn, J. S., Rice, E. J., et al., (2009) High-yield cell-free protein production from P-gel, Nat. Protoc. 4, 1759-1770.
11. Park, N., Um, S. H., Funabashi, H., et al., (2009) A cell-free protein-producing gel, Nat. Mater. 8, 432-437.
12. Kim, T. W., Kim, H. C., Oh, I. S., et al.,(2008) A highly effi cient and economical cell-free protein synthesis system using the S12 extract of Escherichia coli, Biotechnol. Bioprocess Eng. 13, 464-469.
13. Shimizu, Y., Inoue, A., Tomari, Y., et al., (2001) Cell-free translation reconstituted with purifi ed components, Nat. Biotechnol. 19, 751-755. (Pubitemid 32729537)
14. Madin, K., Sawasaki, T., Ogasawara, T., et al.,(2000) A highly effi cient and robust cellfree protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes, Proc. Natl. Acad. Sci. USA 97, 559-564. (Pubitemid 30070348)
15. Erickson, A. H., and Blobel, G. (1983) Cellfree translation of messenger RNA in a wheat germ system, Meth. Enzymol. 96, 38-50.
16. Shirokov VA., Kommer, A., Kolb, V.A., et al., (2007) Continuous-exchange proteinsynthesizing systems, In Vitro Transcription and Translation Protocols (Second Edition), 19-55. Humana Press.
17. Kim, D.M., and Swartz, J.R. (1999) Prolonging cell-free protein synthesis with a novel ATP regeneration system, Biotechnol. Bioeng. 66, 180-188. (Pubitemid 30010342)