Single cell DNA damage/repair assay using HaloChip

Analytical Chemistry

Volumn 84, Issue 2, 2012, Pages 1112-1116

  Qiao, Yong ^a   Wang, Chaoming ^a   Su, Ming ^a   Ma, Liyuan ^a  

^a UNIVERSITY OF CENTRAL FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOMATED ANALYSIS; AUTOMATED DATA ANALYSIS; CELL ARRAY; CELL FUNCTIONS; CELL POPULATIONS; COMET ASSAYS; DNA DAMAGES; DNA DAMAGING AGENTS; DNA FRAGMENT; ETHIDIUM BROMIDE; LABOR-INTENSIVE OPERATIONS; LOW SENSITIVITY; MAMMALIAN CELLS; MICRO WELLS; MICROFABRICATED; MOLECULAR LEVELS; RADICAL ATTACKS; SINGLE CELLS; SINGLE-CELL LEVEL; X RAY IRRADIATION;

BROMINE COMPOUNDS; CELL CULTURE; CELL PROLIFERATION; DNA; FREE RADICALS; GELS; MAMMALS;

CELLS;

SILICON;

ARTICLE; BIOASSAY; BONE TUMOR; CELL NUCLEUS; CHEMISTRY; COMET ASSAY; COMPARATIVE STUDY; DNA DAMAGE; DNA REPAIR; FLUORESCENCE; GENETICS; HELA CELL; HUMAN; METABOLISM; OSTEOSARCOMA; RADIATION EXPOSURE; TISSUE MICROARRAY; TUMOR CELL CULTURE; X RAY;

BIOLOGICAL ASSAY; BONE NEOPLASMS; CELL NUCLEUS; COMET ASSAY; DNA DAMAGE; DNA REPAIR; FLUORESCENCE; HELA CELLS; HUMANS; OSTEOSARCOMA; SILICON; TISSUE ARRAY ANALYSIS; TUMOR CELLS, CULTURED; X-RAYS;

EID: 84862944489     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac202773g     Document Type: Article

References (24)

1. Lindahl, T. Instability and decay of the primary structure of DNA Nature 1993, 362, 709
2. Friedberg, E. C. DNA damage and repair Nature 2003, 421, 436
3. Norbury, C. J.; Hickson, I. D. Cellular responses to DNA damage Annu. Rev. Pharmacol. Toxicol. 2001, 41, 367
4. Wilson, D. M. I.; Bohr, V. A.; McKinnon, P. J. DNA damage, DNA repair, ageing and age-related disease Mech. Ageing Dev. 2008, 129, 349
5. Caldecott, K. W. Single-strand break repair and genetic disease Nat. Rev. Genet. 2008, 9, 619
6. Hoeijmakers, J. H. J. DNA damage, aging, and cancer N. Engl. J. Med. 2009, 361, 1475
7. Ahnstrom, G. Techniques to measure DNA single-strand breaks in cells: a review Int. J. Radiat. Biol. 1988, 54, 695
8. Kumari, S.; Rastogi, R. P.; Singh, K. L.; Sinhg, S. P.; Sinha, R. P. DNA damage: detection strategies EXCLI J. 2008, 7, 44
9. Kumar, A.; Tyagi, M. B.; Jha, P. N. Evidences showing ultraviolet-B radiation-induced damage of DNA in cyanobacteria and its detection by PCR assay Biochem. Biophys. Res. Commun. 2004, 318, 1025
10. Jenner, A.; England, T. G.; Aruoma, O. I.; Halliwell, B. Measurement of oxidative DNA damage by gas chromatography-mass spectrometry: ethanethiol prevents artifactual generation of oxidized DNA Bases Biochem. J. 1998, 331, 365
11. Murthy, S. K.; Demetrick, D. J. New approaches to fluorescence in situ hybridization Methods Mol. Biol. 2006, 319, 237
12. 2+ Anal. Chem. 2001, 73, 4780
13. Wang, J.; Rivas, G.; Ozsoz, M.; Grant, D. H.; Cai, X.; Parrado, C. Microfabricated electrochemical sensor for the detection of radiation-induced DNA damage Anal. Chem. 1997, 69, 1457
14. Olive, P. L.; Banáth, J. P. The comet assay: a method to measure DNA damage in individual cells Nat. Protoc. 2006, 1, 23
15. Collins, A. R. The comet assay for DNA damage and repair Mol. Biotech. 2004, 26, 249
16. Olive, P. L.; Banath, J. P.; Durand, R. E. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay Radiat. Res. 1990, 122, 86
17. Heepchantree, W.; Paratasilpin, T.; Kangwanpong, D. A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence J. Toxicol. Environ. 2006, 69, 1071
18. Kumaravel, T. S.; Vilhar, B.; Faux, S. P.; Jha, A. N. Comet assay measurements: a perspective Cell Biol. Toxicol. 2009, 25, 53
19. Wood, K. K.; Weingeist, D. M.; Bhatia, S. N.; Engelward, B. P. Single cell trapping and DNA damage analysis using microwell arrays Proc. Natl. Acad. Sci. U.S.A. 2010, 107, 10008
20. Sestili, P.; Martinelli, C.; Stocchi, V. The fast halo assay: an improved method to quantify genomic DNA strand breakage at the single-cell level Mutat. Res. 2006, 607, 205
21. Wilson, K.; Stancescu, M.; Das, M.; Rumsey, J.; Hickman, J. Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture J. Vac. Sci. Technol., B 2011, 29, 45
22. Lan, S.; Veiseh, M.; Zhang, M. Surface modification of silicon and gold-patterned silicon surfaces for improved biocompatibility and cell patterning selectivity Biosens. Bioelectron. 2005, 20, 1697
23. Marnett, L. J. Oxyradicals and DNA damage Carcinogenesis 2000, 21, 361
24. Blakely, E.; Chang, P.; Lommel, L.; Bjornstad, K.; Dixon, M.; Tobias, C.; Kumar, K.; Blakely, W. F. Cell-cycle radiation response: role of intracellular factors Adv. Space Res. 1989, 9, 177