Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

Biotechnology and Bioprocess Engineering

Volumn 16, Issue 6, 2011, Pages 1173-1179

  Choi, Su Lim ^a,b   Lee, Sang Jun ^a   Ha, Jae Seok ^b   Song, Jae Jun ^a   Rhee, Young Ha ^a   Lee, Seung Goo ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

Cellulose binding domain (CBD); Escherichia coli; Immobilization; Protein particles; glucuronidase; glycosidase

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; CELLS; CELLULOSE; CYTOLOGY; ESCHERICHIA COLI; RADIOACTIVE WASTE VITRIFICATION;

CELLULOSE BINDING DOMAINS; ESCHERICHIA COLI CELLS; GLUCURONIDASE; GLYCOSIDASES; IMMOBILIZED BIOCATALYSTS; INSOLUBLE AGGREGATES; PROTEIN PARTICLES; THERMUS CALDOPHILUS;

PROTEINS;

ARGININE; BETA GLUCURONIDASE CELLULOSE BINDING DOMAIN FUSION PROTEIN; BETA GLYCOSIDASE CELLULOSE BINDING DOMAIN FUSION PROTEIN; BINDING PROTEIN; CELLULOSE BINDING DOMAIN PROTEIN; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; BETA SHEET; BIOCATALYSIS; CELLULOMONAS; CELLULOMONAS FIMI; CONTROLLED STUDY; CROSS LINKING; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; NONHUMAN; PHYSICAL CHEMISTRY; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; PROTEIN LOCALIZATION; PROTEIN SECONDARY STRUCTURE; REPEATED BATCH FERMENTATION; ULTRASOUND;

CELLULOMONAS FIMI; ESCHERICHIA COLI; THERMUS CALDOPHILUS;

EID: 84862923473     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-011-0336-8     Document Type: Article

References (25)

1. Koeller, K. M. and C. H. Wong (2001) Enzymes for chemical synthesis. Nature 409:232-240. (Pubitemid 32146169)
2. Mateo, C., J. M. Palomo, G. Femandez-Lorente, J. M. Guisan, and R. Femandez-Lafuente (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enz. Microb. Technol. 40:1451-1463. (Pubitemid 46482654)
3. Sheldona, R. A. (2007) Enzyme immobilization: The quest for optimum performance. Adv. Synth. Catal. 349:1289-1307.
4. Moldes, C., P. Garcia, J. L. Garcia, and M. A. Prieto (2004) In vivo immobilization of fusion proteins on bioplastics by the novel tag BioF. Appl. Environ. Microbiol. 70:3205-3212. (Pubitemid 38745860)
5. Peters, V. and B. H. Rehm (2006) In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase. Appl. Environ. Microbiol. 72:1777-1783.
6. Steinmann, B., A. Christmann, T. Heiseler, J. Fritz, and H. Kolmar (2010) In vivo enzyme immobilization by inclusion body display. Appl. Environ. Microbiol. 76:5563-5569.
7. Potter, M., H. Muller, F. Reinecke, R. Wieczorek, F. Fricke, B. Bowien, B. Friedrich, and A. Steinbuchel (2004) The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha. Microbiol. 150:2301-2311. (Pubitemid 39117642)
8. Kane, J. F. and D. L. Hartley (1988) Formation of recombinam protein inclusion bodies in Escherichia coli. Trends Biotechnol. 6:95-101.
9. Thomas, J. G. and F. Baneyx (1996) Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing Heat-shock proteins. J. Biol. Chem. 271:11141-11147. (Pubitemid 26155943)
10. Garcia-Fruitos, E., N. Gonzalez-Montalban, M. Morell, A. Vera, R. M. Ferraz, A. Aris, S. Ventura, and A. Villaverde (2005) Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins. Microb. Cell Fact. 4:27.
11. Yeon, J.-H. and K.-H. Jung (2010) Operation of Packed-bed immobilized cell reactor featuring active β-galactorsidase inclusion Body-containing recombinant Escherichia coli cells. Biotechnol. Bioproc. Eng. 15:822-829.
12. Carrió, M., N. González-Montalbán, A. Vera, A. Villaverde, and S. Ventura (2005) Amyloid-like properties of bacterial inclusion bodies. J. Mol. Biol. 347:1025-1037. (Pubitemid 40387450)
13. Garcia-Fruitos, E., A. Aris, and A. Villaverde (2007) Localization of functional polypeptides in bacterial inclusion bodies. Appl. Environ. Microbiol. 73:289-294. (Pubitemid 46079961)
14. Nahalka, J. and B. Nidetzky (2007) Fusion to a pull-down domain: A novel approach of producing Trigonopsis variabilis D-amino acid oxidase as insoluble enzyme aggregates. Biotechnol. Bioeng. 97:454-461. (Pubitemid 46848060)
15. Din, N., I. J. Forsythe, L. D. Burtnick, N. R. Gilkes, R. C. J. Miller, R. A. Warren, and D. G. Kilburn (1994) The cellulosebinding domain of endoglucanase A (CenA) from Cellulomonas fimi: Evidence for the involvement of tryptophan residues in binding. Mol. Microbiol. 11:747-755. (Pubitemid 24197467)
16. Tormo, J., R. Lamed, A. J. Chirino, E. Morag, E. A. Bayer, Y. Shoham, and T. A. Steitz (1996) Crystal structure of a bacterial family-III cellulose-binding domain: A general mechanism for attachment to cellulose. EMBO J. 15:5739-5751. (Pubitemid 26375495)
17. Xu, G. Y., E. Ong, N. R. Gilkes, D. G. Kilburn, D. R. Muhandiram, M. Harris-Brandts, J. P. Carver, L. E. Kay, and T. S. Harvey (1995) Solution structure of a cellulose-binding domain from Cellulomonas fimi by nuclear magnetic resonance spectroscopy. Biochem. 34:6993-7009.
18. Ha, J.-S., Y.-M. Lee, S.-L. Choi, J. J. Song, C.-S. Shin, J.-H. Kim, and S.-G. Lee (2008) Thermostable beta-glycosidase-CBD fusion protein for biochemical analysis of cotton scouring efficiency. J. Microbiol. Biotechnol. 18:443-448.
19. Nilsson, M. R. (2004) Techniques to study amyloid fibril formation in vitro. Methods. 34:151-160. (Pubitemid 38993215)
20. Jakobs, S., V. Subramaniam, A. Schonle, T. M. Jovin, and S. W. Hell (2000) EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy. FEBS Lett. 479:131-135. (Pubitemid 30616420)
21. Singh, S. M. and A. K. Panda (2005) Solubilization and refolding of bacterial inclusion. body proteins. J. Biosci. Bioeng. 99:303-310. (Pubitemid 40740579)
22. Kim, H. M., B. S. Park, J. I. Kim, S. E. Kim, J. Lee, S. C. Oh, P. Enkhbayar, N. Matsushima, H. Lee, O. J. Yoo, and J. O. Lee (2007) Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran. Cell. 130:906-917. (Pubitemid 47332573)
23. Khurana, R., C. Coleman, C. Ionescu-Zanetti, S. A. Carter, V. Krishna, R. K. Grover, R. Roy, and S. Singh (2005) Mechanism of thioflavin T binding to amyloid fibrils. J. Struct. Biol. 151:229-238. (Pubitemid 41338730)
24. Ami, D., A. Natalello, G. Taylor, G. Tonon, and S. Maria Doglia (2006) Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy. Biochim. Biophys. Acta. 1764:793-799.
25. Umetsu, M., K. Tsumoto, K. Ashish, S. Nitta, Y. Tanaka, T. Adschiri, and I. Kumagai (2004) Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins. FEBS Lett. 557:49-56. (Pubitemid 38115868)