Embryotoxicity assessment of developmental neurotoxicants using a neuronal endpoint in the embryonic stem cell test

Journal of Applied Toxicology

Volumn 32, Issue 8, 2012, Pages 617-626

  Baek, Dae Hyun ^a,b   Kim, Tae Gyun ^a   Lim, Hwa Kyung ^a   Kang, Jin Wook ^a   Seong, Su Kyoung ^a   Choi, Seung Eun ^a   Lim, So Yun ^a   Park, Sung Hee ^a   Nam, Bong hyun ^a   Kim, Eun Hee ^a   Kim, Mun Sin ^a   Park, Kui Lea ^a  

^a Korea Food and Drug Administration   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Arsenic; Developmental neurotoxicity; Embryonic stem cell test; Methylmercury; Valproic acid

Indexed keywords

ARSENATE SODIUM; ARSENITE SODIUM; BIOLOGICAL MARKER; ISONIAZID; MESSENGER RNA; METHYLMERCURY; MICROTUBULE ASSOCIATED PROTEIN 2; NEUROTOXIN; PROTEIN TUJ 1; UNCLASSIFIED DRUG; VALPROIC ACID;

ANIMAL CELL; ARTICLE; CELL ADHESION; CLASSIFICATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DEVELOPMENTAL TOXICITY; EMBRYONIC STEM CELL; EMBRYONIC STEM CELL TEST; EMBRYOTOXICITY; FLOW CYTOMETRY; HEART MUSCLE CELL; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE TEST; MONOCULTURE; MOUSE; NERVE CELL; NERVE CELL DIFFERENTIATION; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; SCREENING TEST; TOXICITY TESTING;

ANIMALS; ARSENATES; ARSENITES; BALB 3T3 CELLS; CELL DIFFERENTIATION; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; EMBRYONIC STEM CELLS; ENDPOINT DETERMINATION; LETHAL DOSE 50; METHYLMERCURY COMPOUNDS; MICE; NEURONS; REPRODUCIBILITY OF RESULTS; SODIUM COMPOUNDS; TOXICITY TESTS; VALPROIC ACID;

EID: 84862901631     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.1747     Document Type: Article

References (35)

1. Bremer S, Hartung T. 2004. The use of embryonic stem cells for regulatory developmental toxicity testing in vitro - the current status of test development. Curr. Pharm. Des. 10: 2733-2747.
2. Burbacher TM, Rodier PM, Weiss B. 1990. Methylmercury developmental neurotoxicity: a comparison of effects in humans and animals. Neurotoxicol. Teratol. 12: 191-202.
3. Buzanska L, Sypecka J, Nerni-Molteni S, Compagnoni A, Hogber HT, del Torchio R, Domanska-Janik K, Zimmer J, Coecke S. 2009. A human stem cell-based model for identifying adverse effects of organic and inorganic chemicals on the developing nervous system. Stem Cells 27: 2591-2601.
4. Castoldi AF, Coccini T, Ceccatelli S, Manzo L. 2001. Neurotoxicity and molecular effects of methylmercury. Brain Res. Bull. 55: 197-203.
5. Castoldi AF, Onishchnko N, Johansson C, Coccini T, Roda E, Vahter M, Ceccatelli S, Manzo L. 2008. Neurodevelopmental toxicity of methylmercury: laboratory animal data and their contribution to human risk assessment. Regul. Toxicol. Pharmacol. 51: 215-229.
6. Ceccatelli S, Dare E, Moors M. 2010. Methylmercury-induced neurotoxicity and apoptosis. Chem. Biol. Interact. 188: 301-308.
7. Dhar P, Mohari N, Mehra RD. 2007. Preliminary morphological and morphometric study of rat cerebellum following sodium arsenite exposure during rapid brain growth (RBG) period. Toxicology 234: 10-20.
8. Festag M, Viertel B, Steinberg P, Sehner C. 2007. An in vitro embryotoxicity assay based on the disturbance of the differentiation of murine embryonic stem cells into endothelial cells. II. Testing compounds. Toxicol. Vitro 21: 1631-1640.
9. Genschow E, Spielmann H, Scholz G, Pohl I, Seiler A, Clemann N, Bremer S, Becker K. 2004. Validation of the embryonic stem cell test in the international ECVAM validation study on three in vitro embryotoxicity tests. Altern. Lab. Anim. 32: 209-244.
10. Grandjean P, Landrigan PJ. 2006. Developmental neurotoxicity of industrial chemicals. Lancet 368: 2167-2178.
11. Grandjean P, Satoh H, Murata K, Eto K. 2010. Adverse effects of methylmercury: environmental health research implications. Environ. Health Perspect. 118: 1137-1145.
12. Hogberg H, Kinsner-Ovaskainen A, Hartung T, Coecke S, Bal-Price AK. 2009. Gene expression as a sensitive endpoint to evaluate cell differentiation and maturation of the developing central nervous system in primary cultures of rat cerebellar granule cells (CGCs) exposed to pesticides. Toxicol. Appl. Pharmacol. 235: 268-286.
13. Hogberg H, Kinsner-Ovaskainen A, Coecke S, Hartung T, Bal-Price AK. 2010. mRNA expression is a relevant tool to identify developmental neurotoxicants using an in vitro approach. Toxicol. Sci. 113: 95-115.
14. Iqbal MM, Sohhan T, Mahmud SZ. 2001. The effects of lithium, valproic acid, and carbamazepine during pregnancy and lactation. J. Toxicol. Clin. Toxicol. 39: 381-392.
15. Kuwagata M, Ogawa T, Shioda S, Nagata T. 2009. Observation of fetal brain in a rat valproate-induced autism model. Int. J. Dev. Neurosci. 27: 399-405.
16. Lee SH, Lumelsky N, Studer L, Auerbach JM, McKay RD. 2000. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat. Biotechnol. 18: 675-679.
17. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4): 402-408.
18. Ornoy A. 2009. Valproic acid in pregnancy: how much are we endangering the embryo and fetus? Reprod. Toxicol. 28: 1-10.
19. Rocha RA, Gimeno-Alcaniz JV, Martin-Ibanez R, Canals JM, Velez D, Devesa V. 2011. Arsenic and fluoride induce neural progenitor cell apoptosis. Toxicol. Lett. 203: 237-244.
20. Rodríguez VM, Carrizales L, Mendoza MS, Fajardo OR, Giordano M. 2002. Effects of sodium arsenite exposure on development and behavior in the rat. Neurotoxicol. Teratol. 24: 743-750.
21. Scholz G, Genschow E, Pohl I, Bremer S, Paparella M, Raabe H, Southee J, Spielmann H. 1999. Prevalidation of the embryonic stem cell test (EST) - a new in vitro embryotoxicity test. Toxicol. Vitro 13: 675-681.
22. Seiler A, Buesen R, Visan A, Spielmann H. 2006. Embryonic stem cell protocols. In Methods in Molecular Biology, Turksen K. (ed.). Humana Press: Totowa, NJ; 371-395.
23. Sergent-Tanguy S, Chagneau C, Neveu I, Naveilhan P. 2003. Fluorescent activated cell sorting (FACS): a rapid reliable method to estimate the number of neurons in a mixed population. J. Neurosci. Meth. 129: 73-79.
24. Spielmann H. 2005. The embryonic stem cell test (EST), an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. Toxicol. Appl. Pharmacol. 207: 375-380.
25. Studer L. 2006. The nervous system. In Essentials of Stem Cell Biology, Lanza R, Gearheart J, Hogan B, Melton D, Petersen R, Thomas ED, Thomson J, West M. (eds). Academic Press: San Diego, CA; 149-156.
26. Stummann TC, Hareng L, Bremer S. 2007. Embryotoxicity hazard assessment of methylmercury and chromium using embryonic stem cells. Toxicology 242: 130-143.
27. Stummann TC, Hareng L, Bremer S. 2008. Embryotoxicity hazard assessment of cadmium and arsenic compounds using embryonic stem cells. Toxicology 252: 118-122.
28. Stummann TC, Hareng L, Bremer S. 2009. Hazard assessment of methylmercury toxicity to neuronal induction in embryogenesis using embryonic stem cells. Toxicology 257: 117-126.
29. Sun Y, Long D, Li W, Hou W, Wu Y, Shen J. 2010. Effects of avermectins on neurite outgrowth in differentiating mouse neuroblastoma N2a cells. Toxicol. Lett. 192: 206-211.
30. Tamm C, Duckworth J, Hermanson O, Ceccatelli S. 2006. High susceptibility of neural stem cells to methylmercury toxicity: effects on cell survival and neuronal differentiation. J. Neurochem. 97: 69-78.
31. Theunissen PT, Schulpen SHW, van Dartel DAM, Hermsen SAB, van Schooten FJ, Piersma AH. 2010. An abbreviated protocol for multilineage neural differentiation of murine embryonic stem cells and its perturbation by methyl mercury. Reprod. Toxicol. 29: 383-392.
32. Toumadje A, Kusumoto KI, Parton A, Mericko P, Dowell L, MaG, Chen L, Barnes DW, Sato JD. 2003. Pluripotent differentiation in vitro of murine ES-D3 embryonic stem cells. In Vitro Cell. Dev. Biol. Animal 39: 449-453.
33. Yamashita N, Doi M, Nishio M, Hojo H, Tanaka M. 1972. Recent observations of Kyoto children poisoned by arsenic-tainted 'Morinaga dry milk' [in Japanese]. Nippon Eiseigaku Zashi 27: 364-399.
34. Ying QL, Stravridis M, Griffiths D, Li M, Smith A. 2003. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotech. 21: 183-186.
35. zur Nieden NI, Kempka G, Ahr HJ. 2004. Molecular multiple endpoint embryonic stem cell test - a possible approach to test for the teratogenic potential of compound. Toxicol. Appl. Pharmacol. 194: 257-269.