The role of reactive oxygen species in silicon dioxide nanoparticle-induced cytotoxicity and DNA damage in HaCaT cells

Molecular Biology Reports

Volumn 39, Issue 4, 2012, Pages 4915-4925

  Gong, Chunmei ^a,b   Tao, Gonghua ^b   Yang, Linqing ^b   Liu, Jianjun ^b   He, Haowei ^c   Zhuang, Zhixiong ^b  

^a ZHENGZHOU UNIVERSITY   (China)

^b SHENZHEN CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

^c SHENZHEN UNIVERSITY   (China)

Author keywords

Flow cytometry immunofluorescence; Reactive oxygen species (ROS); Silicon dioxide nanoparticles

Indexed keywords

8 HYDROXYDEOXYGUANOSINE; HISTONE H2AX; REACTIVE OXYGEN METABOLITE; SILICON DIOXIDE; 8-HYDROXY-2'-DEOXYGUANOSINE; DEOXYGUANOSINE; DRUG DERIVATIVE; H2AFX PROTEIN, HUMAN; HISTONE; NANOPARTICLE;

APOPTOSIS; ARTICLE; CELL ASSAY; CELL LEVEL; CELL MEDIATED CYTOTOXICITY; CELL VIABILITY; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOARCHITECTURE; CYTOTOXICITY TEST; DNA DAMAGE; HUMAN; HUMAN CELL; KERATINOCYTE; NANOANALYSIS; OXIDATIVE STRESS; PARTICLE SIZE; CELL DEATH; CELL LINE; CELL SHAPE; CELL SURVIVAL; CYTOLOGY; DRUG EFFECT; INTRACELLULAR SPACE; METABOLISM; ULTRASTRUCTURE;

CELL DEATH; CELL LINE; CELL SHAPE; CELL SURVIVAL; COMET ASSAY; DEOXYGUANOSINE; DNA DAMAGE; HISTONES; HUMANS; INTRACELLULAR SPACE; KERATINOCYTES; NANOPARTICLES; OXIDATIVE STRESS; PARTICLE SIZE; REACTIVE OXYGEN SPECIES; SILICON DIOXIDE;

EID: 84862893488     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-1287-z     Document Type: Article

References (22)

1. Stix G (2001) Little big science. Nanotechnology 285:32-37
2. Kipen HM, Laskin DL (2005) Smaller is not always better: Nanotechnology yields nanotoxicology. Am J Physiol Lung Cell Mol Physiol 289:L696-L697
3. Nel A, Xia T, Mädler L (2006) Toxic potential of materials at the nanolevel. Science 311:622-627
4. Borm PJ, Robbins D, Haubold S, Kuhlbusch T, Fissan H, Donaldson K, Schins R, Stone V, Kreyling W, Lademann J, Krutmann J, Warheit D, Oberdorster E (2006) The potential risks of nanomaterials: A review carried out for ECETOC. Part Fibre Toxicol 3:11
5. Rothen-Rutishauser BM, Schürch S, Haenni B, Kapp N, Gehr P (2006) Interaction of fine particles and nanopartcles with red blood cells visualized with advanced microscopic technique. Environ Sci Technol 40:353-359
6. IRAC (1996) Monographs on the evaluation of chemicals to humans. Lyon, IRAC, 10
7. Giraldo AM, Lynn JW, Purpera MN, Godke RA, Bondioli KR (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Mol Reprod Dev 74:1514-1524
8. Espada J, Ballestar E, Esteller M (2001) Qualitative determination of 5-methylcytosine and other components of the DNA methylation machinery: Immunofluorescence and chromatin immunoprecipitation. In: Esteller M (ed) DNA methylation: Approaches, methods, and applications. CRC press, Boca Raton, pp 122-126
9. Xu B, Kim S, Kastan MB (2001) Involvement of Brca1 in S phase and G2-phase checkpoints after ionizing irradiation. Mol Cell Biol 21:3445-3450
10. Tao GH, Yang LQ, Gong CM, Huang HY, Liu JD, Liu JJ, Yuan JH, Chen W, Zhuang ZX (2009) Effect of PARP-1 deficiency on DNA damage and repair in human bronchial epithelial cells exposed to Benzo(a)pyrene. Mol Biol Rep 36(8):2413-2422
11. Olive PL, Durand RE (1992) Detection of hypoxic cells in a murine tumor with the use of the comet assay. J Natl Cancer Inst 84:707-711
12. Fujiwara K, Suematsu H, Kiyomiya E, Aoki M, Sato M, Moritoki N (2008) Size-dependent toxicity of SiO2 nano-particles to Chlorella kessleri. J Environ Sci Health A Tox Hazard Subst Environ Eng 43:1167-1173
13. Rahman IA, Vejayakumaran P, Sipaut SC, Ismail J, Chee KC (2009) Size-dependent physicochemical and optical properties of silica nanoparticles. Mater Chem Phys 114:328-332
14. Wang F, Gao F, Lan M, Yuan H, Huang Y, Liu J (2009) Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells. Toxicol in Vitro 23:808-815
15. Eom HJ, Choi J (2009) Oxidative stress of silica nanoparticles in human bronchial epithelial cell, Beas-2B. Toxicol In Vitro 23:1326-1332
16. Dostert C, Petrilli V, Van Bruggen R, Steele C, Mossman BT, Tschopp J (2008) Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science 320:674-677
17. Ames BN (1983) Dietary carcinogens, anticarcinogens (oxygen radicals and degenerative diseases). Science 221:1256-1264
18. Rojas E, Lopez MC, Valverde M (1999) Single cell gel electrophoresis assay: Methodology and applications. J Chromatogr B Biomed Sci Appl 722:225-254
19. Motoyama N, Naka K (2004) DNA damage tumor suppressor genes and genomic instability. Curr Opin Genet Dev 14:11-16
20. Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622-627
21. Nabeshil H, Yoshikawai T, Matsuyamai K (2011) Amorphous nanosilica induce endocytosis dependent ROS generation and DNA damage in human keratinocytes. Part Fibre Toxicol 8:1
22. Chen M, von Mikecz A (2005) Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles. Exp Cell Res 305:51-62