Purification and characterization of the human γ-secretase activating protein

Biochemistry

Volumn 51, Issue 25, 2012, Pages 5153-5159

  Deatherage, Catherine L ^a   Hadziselimovic, Arina ^a   Sanders, Charles R ^a  

^a VANDERBILT UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALZHEIMER'S DISEASE; AMYLOID PRECURSOR PROTEINS; AVERAGE LIFE EXPECTANCY; BIOCHEMICAL CHARACTERIZATION; C-TERMINAL DOMAINS; CANCER DRUG; ELEVATED LEVEL; GLEEVEC; IMATINIB; INCLUSION BODIES; MICELLE SOLUTION; NEUROLOGICAL DISORDERS; REFOLDING; SECRETASES; TERTIARY STRUCTURES; TRANSMEMBRANES;

GLYCOPROTEINS; PURIFICATION; SOAPS (DETERGENTS); UREA;

RECOMBINANT PROTEINS;

AMYLOID PRECURSOR PROTEIN; GAMMA SECRETASE ACTIVATING PROTEIN; IMATINIB; PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; DRUG PROTEIN BINDING; MICELLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; INCLUSION BODIES; PLASMIDS; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEINS; RECOMBINANT FUSION PROTEINS; THIOREDOXINS;

BACTERIA (MICROORGANISMS);

EID: 84862882938     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300605u     Document Type: Article

References (15)

1. Alzheimer's Association (2012) Alzheimer's disease facts and figures Alzheimer's & Dementia: The Journal of the Alzheimer's Association 8, 131-168
2. Selkoe, D. J. (1991) The molecular pathology of Alzheimer's disease Neuron 6, 487-498
3. Hardy, J. and Selkoe, D. J. (2002) The Amyloid Hypothesis of Alzheimer's Disease: Progress and Problems on the Road to Therapeutics Science 297, 353-356 (Pubitemid 34790756)
4. Esler, W. P., Kimberly, W. T., Ostaszewski, B. L., Ye, W., Diehl, T. S., Selkoe, D. J., and Wolfe, M. S. (2002) Activity-dependent isolation of the presenilin-γ-secretase complex reveals nicastrin and a γ substrate Proc. Natl. Acad. Sci. U.S.A. 99, 2720
5. Kimberly, W. T., LaVoie, M. J., Ostaszewski, B. L., Ye, W., Wolfe, M. S., and Selkoe, D. J. (2003) γ-Secretase is a membrane protein complex comprised of presenilin, nicastrin, Aph-1, and Pen-2 Proc. Natl. Acad. Sci. U.S.A. 100, 6382
6. De Strooper, B. (2003) Aph-1, Pen-2, and Nicastrin with Presenilin generate an active γ-secretase complex Neuron 38, 9-12 (Pubitemid 36423348)
7. Kopan, R. and Ilagan, M. X. (2004) γ-Secretase: Proteasome of the membrane? Nat. Rev. Mol. Cell Biol. 5, 499-504
8. He, G., Luo, W., Li, P., Remmers, C., Netzer, W. J., Hendrick, J., Bettayeb, K., Flajolet, M., Gorelick, F., Wennogle, L. P., and Greengard, P. (2010) γ-Secretase activating protein is a therapeutic target for Alzheimer's disease Nature 467, 95-98
9. Netzer, W. J., Dou, F., Cai, D., Veach, D., Jean, S., Li, Y., Bornmann, W. G., Clarkson, B., Xu, H., and Greengard, P. (2003) Gleevec inhibits β-amyloid production but not Notch cleavage Proc. Natl. Acad. Sci. U.S.A. 100, 12444-12449 (Pubitemid 37271579)
10. Satoh, J., Tabunoki, H., Ishida, T., Saito, Y., and Arima, K. (2012) Immunohistochemical characterization of γ-secretase activating protein expression in Alzheimer's disease brains Neuropathol. Appl. Neurobiol. 38, 132-141
11. Beel, A. J., Mobley, C. K., Kim, H. J., Tian, F., Hadziselimovic, A., Jap, B., Prestegard, J. H., and Sanders, C. R. (2008) Structural studies of the transmembrane C-terminal domain of the amyloid precursor protein (APP): Does APP function as a cholesterol sensor? Biochemistry 47, 9428-9446
12. Sanders, C. R. and Sonnichsen, F. (2006) Solution NMR of membrane proteins: Practice and challenges Magn. Reson. Chem. 44, S24-S40
13. LaVallie, E. R., DiBlasio, E. A., Kovacic, S., Grant, K. L., Schendel, P. F., and McCoy, J. M. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Nat. Biotechnol. 11, 187-193 (Pubitemid 23056045)
14. Sorensen, H. P. and Mortensen, K. K. (2005) Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli Microb. Cell Fact. 4, 1
15. Louis-Jeune, C., Andrade-Navarro, M. A., and Perez-Iratxeta, C. (2012) Prediction of protein secondary structure from circular dichroism using theoretically derived spectra Proteins 80, 374-381