Development of real-time assays for impedance-based detection of microbial double-stranded DNA targets: Optimization and data analysis

Biosensors and Bioelectronics

Volumn 35, Issue 1, 2012, Pages 87-93

  Ghindilis, Andrei L ^a   Smith, Maria W ^b   Messing, Dean S ^a   Haynes, Vena N ^b   Middleton, George B ^a   Schwarzkopf, Kevin R ^a   Campbell, Carmen E ^a   Zhan, Changqing ^a   Ulrich, Bruce ^a   Frasier, Michael J ^a   Schuele, Paul J ^a   Evans, David R ^a   Sezan, Ibrahim ^a   Hartzell, John W ^a   Simon, Holly M ^b  

^a SHARP LABORATORIES OF AMERICA   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Double stranded DNA detection; Impedance sensor array; Real time label free detection

Indexed keywords

ARRAY SURFACE; ASSAY DEVELOPMENT; ASSAY OPTIMIZATION; BINDING CURVES; BUFFER CONCENTRATIONS; DOUBLE STRANDED DNA; FIXED FREQUENCY; HIGH CONFIDENCE; IMPEDANCE SENSORS; INTEGRATED DATA; LABEL FREE; LABEL-FREE DETECTION; NON-SPECIFIC BINDING; OLIGONUCLEOTIDE PROBES; SHARP LABORATORIES; SIGNAL RESPONSE; TARGET BINDING;

DNA; KINETIC PARAMETERS; OLIGONUCLEOTIDES; OPTIMIZATION; PROBES; SENSORS; SIGNAL DETECTION; SURFACE ACTIVE AGENTS;

ASSAYS;

BACTERIAL DNA; DOUBLE STRANDED DNA; SURFACTANT;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DATA ANALYSIS; DNA DETERMINATION; DNA HYBRIDIZATION; ELECTRIC POTENTIAL; ENZYME KINETICS; ESCHERICHIA COLI; IMPEDANCE; MICROORGANISM DETECTION; NONHUMAN; OLIGONUCLEOTIDE PROBE; PROCESS OPTIMIZATION; SENSOR; TEMPERATURE;

BACTERIOLOGICAL TECHNIQUES; BASE SEQUENCE; BIOSENSING TECHNIQUES; COMPUTER SYSTEMS; DATA INTERPRETATION, STATISTICAL; DNA, BACTERIAL; ELECTRIC IMPEDANCE; EQUIPMENT REUSE; ESCHERICHIA COLI; GENES, BACTERIAL; MICROBIOLOGICAL TECHNIQUES; POLYMERASE CHAIN REACTION;

EID: 84862818892     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2012.02.021     Document Type: Article

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