RNA editing of the Q/R site of GluA2 in different cultured cell lines that constitutively express different levels of RNA editing enzyme ADAR2

Neuroscience Research

Volumn 73, Issue 1, 2012, Pages 42-48

  Yamashita, Takenari ^a   Tadami, Chieko ^a,b   Nishimoto, Yoshinori ^a,b   Hideyama, Takuto ^a   Kimura, Daisuke ^a,b   Suzuki, Takeshi ^b   Kwak, Shin ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KEIO UNIVERSITY   (Japan)

Author keywords

Adenosine deaminase acting on RNA type 2 (ADAR2); AMPA receptor; Amyotrophic lateral sclerosis (ALS); GluA2; RNA editing; Splicing

Indexed keywords

ADENOSINE DEAMINASE; ADENOSINE DEAMINASE ACTING ON RNA 2; ARGININE; GLUTAMATE RECEPTOR; GLUTAMINE; MESSENGER RNA; MESSENGER RNA PRECURSOR; PROTEIN GLUA2; UNCLASSIFIED DRUG;

ARTICLE; CELL LINE; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RNA EDITING; RNA SPLICING; RNA TRANSPORT;

ADENOSINE DEAMINASE; ANIMALS; ARGININE; CELLS, CULTURED; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLUTAMINE; HELA CELLS; HUMANS; RATS; RECEPTORS, AMPA; RNA EDITING; RNA SPLICING; RNA-BINDING PROTEINS;

EID: 84862777298     PISSN: 01680102     EISSN: 18728111     Source Type: Journal    
DOI: 10.1016/j.neures.2012.02.002     Document Type: Article

References (38)

1. Aizawa H., Sawada J., Hideyama T., Yamashita T., Katayama T., Hasebe N., Kimura T., Yahara O., Kwak S. TDP-43 pathology in sporadic ALS occurs in motor neurons lacking the RNA editing enzyme ADAR2. Acta Neuropathol. 2010, 120:75-84.
2. Bass B.L. RNA editing by adenosine deaminases that act on RNA. Annu. Rev. Biochem. 2002, 71:817-846.
3. Bhalla T., Rosenthal J.J., Holmgren M., Reenan R. Control of human potassium channel inactivation by editing of a small mRNA hairpin. Nat. Struct. Mol. Biol. 2004, 11:950-956.
4. Brusa R., Zimmermann F., Koh D.S., Feldmeyer D., Gass P., Seeburg P.H., Sprengel R. Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in mice. Science 1995, 270:1677-1680.
5. Burnashev N., Monyer H., Seeburg P.H., Sakmann B. Divalent ion permeability of AMPA receptor channels is dominated by the edited form of a single subunit. Neuron 1992, 8:189-198.
6. Carlson N.G., Howard J., Gahring L.C., Rogers S.W. RNA editing (Q/R site) and flop/flip splicing of AMPA receptor transcripts in young and old brains. Neurobiol. Aging 2000, 21:599-606.
7. Chen C.X., Cho D.S., Wang Q., Lai F., Carter K.C., Nishikura K. A third member of the RNA-specific adenosine deaminase gene family, ADAR3, contains both single- and double-stranded RNA binding domains. RNA 2000, 6:755-767.
8. Desterro J.M., Keegan L.P., Lafarga M., Berciano M.T., O'Connell M., Carmo-Fonseca M. Dynamic association of RNA-editing enzymes with the nucleolus. J. Cell Sci. 2003, 116:1805-1818.
9. Gan Z., Zhao L., Yang L., Huang P., Zhao F., Li W., Liu Y. RNA editing by ADAR2 is metabolically regulated in pancreatic islets and beta-cells. J. Biol. Chem. 2006, 281:33386-33394.
10. Hideyama T., Yamashita T., Suzuki T., Tsuji S., Higuchi M., Seeburg P.H., Takahashi R., Misawa H., Kwak S. Induced loss of ADAR2 engenders slow death of motor neurons from Q/R site-unedited GluR2. J. Neurosci. 2010, 30:11917-11925.
11. Higuchi M., Maas S., Single F.N., Hartner J., Rozov A., Burnashev N., Feldmeyer D., Sprengel R., Seeburg P.H. Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2. Nature 2000, 406:78-81.
12. Hume R.I., Dingledine R., Heinemann S.F. Identification of a site in glutamate receptor subunits that controls calcium permeability. Science 1991, 253:1028-1031.
13. Kawahara Y., Ito K., Sun H., Aizawa H., Kanazawa I., Kwak S. Glutamate receptors: RNA editing and death of motor neurons. Nature 2004, 427:801.
14. Kawahara Y., Ito K., Sun H., Ito M., Kanazawa I., Kwak S. Regulation of glutamate receptor RNA editing and ADAR mRNA expression in developing human normal and Down's syndrome brains. Brain Res. Dev. Brain Res. 2004, 148:151-155.
15. Kawahara Y., Ito K., Sun H., Kanazawa I., Kwak S. Low editing efficiency of GluR2 mRNA is associated with a low relative abundance of ADAR2 mRNA in white matter of normal human brain. Eur. J. Neurosci. 2003, 18:23-33.
16. Kim U., Garner T.L., Sanford T., Speicher D., Murray J.M., Nishikura K. Purification and characterization of double-stranded RNA adenosine deaminase from bovine nuclear extracts. J. Biol. Chem. 1994, 269:13480-13489.
17. Kwak S., Hideyama T., Yamashita T., Aizawa H. AMPA receptor-mediated neuronal death in sporadic ALS. Neuropathology 2010, 30:182-188.
18. Kwak S., Kawahara Y. Deficient RNA editing of GluR2 and neuronal death in amyotropic lateral sclerosis. J. Mol. Med. 2005, 83:110-120.
19. Kwak S., Nishimoto Y., Yamashita T. Newly identified ADAR-mediated A-to-I editing positions as a tool for ALS research. RNA Biol. 2008, 5:193-197.
20. Lomeli H., Mosbacher J., Melcher T., Hoger T., Geiger J.R., Kuner T., Monyer H., Higuchi M., Bach A., Seeburg P.H. Control of kinetic properties of AMPA receptor channels by nuclear RNA editing. Science 1994, 266:1709-1713.
21. Maas S., Patt S., Schrey M., Rich A. Underediting of glutamate receptor GluR-B mRNA in malignant gliomas. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:14687-14692.
22. Marcucci R., Brindle J., Paro S., Casadio A., Hempel S., Morrice N., Bisso A., Keegan L.P., Del Sal G., O'Connell M.A. Pin1 and WWP2 regulate GluR2 Q/R site RNA editing by ADAR2 with opposing effects. EMBO J. 2011, 30:4211-4222.
23. Melcher T., Maas S., Herb A., Sprengel R., Higuchi M., Seeburg P.H. RED2, a brain-specific member of the RNA-specific adenosine deaminase family. J. Biol. Chem. 1996, 271:31795-31798.
24. Melcher T., Maas S., Herb A., Sprengel R., Seeburg P.H., Higuchi M. A mammalian RNA editing enzyme. Nature 1996, 379:460-464.
25. Nishikura K. Editing the message from A to I. Nat. Biotechnol. 2004, 22:962-963.
26. Nishimoto Y., Yamashita T., Hideyama T., Tsuji S., Suzuki N., Kwak S. Determination of editors at the novel A-to-I editing positions. Neurosci. Res. 2008, 61:201-206.
27. O'Connell M.A., Gerber A., Keller W. Purification of human double-stranded RNA-specific editase 1 (hRED1) involved in editing of brain glutamate receptor B pre-mRNA. J. Biol. Chem. 1997, 272:473-478.
28. O'Connell M.A., Krause S., Higuchi M., Hsuan J.J., Totty N.F., Jenny A., Keller W. Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase. Mol. Cell. Biol. 1995, 15:1389-1397.
29. Paschen W., Djuricic B. Regional differences in the extent of RNA editing of the glutamate receptor subunits GluR2 and GluR6 in rat brain. J. Neurosci. Methods 1995, 56:21-29.
30. Peng P.L., Zhong X., Tu W., Soundarapandian M.M., Molner P., Zhu D., Lau L., Liu S., Liu F., Lu Y. ADAR2-dependent RNA editing of AMPA receptor subunit GluR2 determines vulnerability of neurons in forebrain ischemia. Neuron 2006, 49:719-733.
31. Riedmann E.M., Schopoff S., Hartner J.C., Jantsch M.F. Specificity of ADAR-mediated RNA editing in newly identified targets. RNA 2008, 14:1110-1118.
32. Rueter S.M., Burns C.M., Coode S.A., Mookherjee P., Emeson R.B. Glutamate receptor RNA editing in vitro by enzymatic conversion of adenosine to inosine. Science 1995, 267:1491-1494.
33. Ryman K., Fong N., Bratt E., Bentley D.L., Ohman M. The C-terminal domain of RNA Pol II helps ensure that editing precedes splicing of the GluR-B transcript. RNA 2007, 13:1071-1078.
34. Sakurai M., Yano T., Kawabata H., Ueda H., Suzuki T. Inosine cyanoethylation identifies A-to-I RNA editing sites in the human transcriptome. Nat. Chem. Biol. 2010, 6:733-740.
35. Sansam C.L., Wells K.S., Emeson R.B. Modulation of RNA editing by functional nucleolar sequestration of ADAR2. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:14018-14023.
36. Sawada J., Yamashita T., Aizawa H., Aburakawa Y., Hasebe N., Kwak S. Effects of antidepressants on GluR2 Q/R site-RNA editing in modified HeLa cell line. Neurosci. Res. 2009, 64:251-258.
37. Schoft V.K., Schopoff S., Jantsch M.F. Regulation of glutamate receptor B pre-mRNA splicing by RNA editing. Nucleic Acids Res. 2007, 35:3723-3732.
38. Takuma H., Kwak S., Yoshizawa T., Kanazawa I. Reduction of GluR2 RNA editing, a molecular change that increases calcium influx through AMPA receptors, selective in the spinal ventral gray of patients with amyotrophic lateral sclerosis. Ann. Neurol. 1999, 46:806-815.