Human CD4 + lymphocytes for antigen quantification: Characterization using conventional flow cytometry and mass cytometry

Cytometry Part A

Volumn 81 A, Issue 7, 2012, Pages 567-575

  Wang, Lili ^a   Abbasi, Fatima ^b   Ornatsky, Olga ^c,d   Cole, Kenneth D ^a   Misakian, Martin ^a   Gaigalas, Adolfas K ^a   He, Hua Jun ^a   Marti, Gerald E ^e   Tanner, Scott ^c,d   Stebbings, Richard ^f  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^c UNIVERSITY OF TORONTO   (Canada)

^d DVS Sciences Inc   (Canada)

^e CENTER FOR DEVICES AND RADIOLOGICAL HEALTH   (United States)

^f NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

Antibody bound per cell; CD4 expression; Cell diameter; Cell membrane intactness; Cryopreserved peripheral blood mononuclear cells; Cyto Trol; CyTOF mass cytometry; Fixation effect; Lyophilized peripheral blood mononuclear cell; Quantitative multiparameter flow cytometry; Whole blood

Indexed keywords

ANTIGEN; FIXATIVE; FORMALDEHYDE; PARAFORMALDEHYDE; POLYMER;

ARTICLE; CALIBRATION; CD4+ T LYMPHOCYTE; CELL MEMBRANE; CELL SEPARATION; CHEMISTRY; FLOW CYTOMETRY; HUMAN; METABOLISM; PERMEABILITY; STAINING; TISSUE FIXATION;

ANTIGENS; CALIBRATION; CD4-POSITIVE T-LYMPHOCYTES; CELL MEMBRANE; CELL SEPARATION; FIXATIVES; FLOW CYTOMETRY; FORMALDEHYDE; HUMANS; PERMEABILITY; POLYMERS; STAINING AND LABELING; TISSUE FIXATION;

EID: 84862766953     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22060     Document Type: Article

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