Site-directed mutagenesis and feedback-resistant N-acetyl-L-glutamate kinase (NAGK) increase Corynebacterium crenatum L-arginine production

Amino Acids

Volumn 43, Issue 1, 2012, Pages 255-266

  Xu, Meijuan ^a   Rao, Zhiming ^a   Dou, Wenfang ^a   Yang, Juan ^a   Jin, Jian ^a   Xu, Zhenghong ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Corynebacterium crenatum; Feedback inhibition; l Arginine production; N Acetyl l glutamate kinase (NAGK); Site directed mutagenesis

Indexed keywords

ARGININE; BACTERIAL ENZYME; N ACETYLGLUTAMIC ACID KINASE; UNCLASSIFIED DRUG;

AMINO ACID SYNTHESIS; ARGB GENE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CELL LEVEL; CONTROLLED STUDY; CORYNEBACTERIUM; CORYNEBACTERIUM CRENATUM; ENZYME ACTIVITY; ENZYME REGULATION; GENE OVEREXPRESSION; NEGATIVE FEEDBACK; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRAIN DIFFERENCE; TRANSGENIC ORGANISM;

AMINO ACID SEQUENCE; ARGININE; CORYNEBACTERIUM; FEEDBACK, PHYSIOLOGICAL; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHOTRANSFERASES (CARBOXYL GROUP ACCEPTOR); PROTEIN STRUCTURE, SECONDARY; SEQUENCE ALIGNMENT;

CORYNEBACTERIUM CRENATUM; CORYNEBACTERIUM SP.;

EID: 84862756774     PISSN: 09394451     EISSN: 14382199     Source Type: Journal    
DOI: 10.1007/s00726-011-1069-x     Document Type: Article

References (28)

1. B Benkert N Quack K Schreiber L Jaensch D Jahn M Schobert 2008 Nitrate-responsive NarX-NarL represses arginine-mediated induction of the Pseudomonas aeruginosa arginine fermentation arcDABC operon Microbiol SGM 154 3053 3060 10.1099/mic.0.2008/018929-0 10.1099/mic.0.2008/018929-0 1:CAS:528:DC%2BD1cXhtlagtbjN
2. B Blombach S Hans B Bathe BJ Eikmanns 2009 Acetohydroxyacid synthase, a novel target for improvement of L-lysine production by Corynebacterium glutamicum Appl Environ Microbiol 75 2 419 427 10.1128/aem.01844-08 19047397 10.1128/AEM.01844-08 1:CAS:528:DC%2BD1MXktlejsb8%3D
3. B Blombach ME Schreiner T Bartek M Oldiges BJ Eikmanns 2008 Corynebacterium glutamicum tailored for high-yield l-valine production Appl Microbiol Biotechnol 79 3 471 479 10.1007/s00253-008-1444-z 18379776 10.1007/s00253-008-1444-z 1:CAS:528:DC%2BD1cXlvF2kurs%3D (Pubitemid 351704103)
4. MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254 942051 10.1016/0003-2697(76)90527-3 1:CAS:528: DyaE28XksVehtrY%3D
5. R Cunin N Glansdorff A Pierard V Stalon 1986 Biosynthesis and metabolism of arginine in bacteria Microbiol Rev 50 3 314 352 0146-0749/86/090314-3902.00/0 3534538 1:CAS:528:DyaL28Xls1yms7g%3D (Pubitemid 16017072)
6. V Elisakova M Patek J Holatko J Nesvera D Leyval J-L Goergen S Delaunay 2005 Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum Appl Environ Microbiol 71 1 207 213 10.1128/AEM.71.1.207-213.2005 15640189 10.1128/AEM.71.1.207-213.2005 1:CAS:528:DC%2BD2MXotlaqtg%3D%3D (Pubitemid 40116269)
7. ML Fernandez-Murga F Gil-Ortiz JL Llacer V Rubio 2004 Arginine biosynthesis in Thermotoga maritima: Characterization of the arginine-sensitive N-acetyl-l-glutamate kinase J Bacteriol 186 18 6142 6149 10.1128/jb.186.18.6142- 6149.2004 15342584 10.1128/JB.186.18.6142-6149.2004 1:CAS:528: DC%2BD2cXns1eisbg%3D (Pubitemid 39187003)
8. ML Fernandez-Murga V Rubio 2008 Basis of arginine sensitivity of microbial N-acetyl-l-glutamate kinases: mutagenesis and protein engineering study with the Pseudomonas aeruginosa and Escherichia coli enzymes J Bacteriol 190 8 3018 3025 10.1128/jb.01831-07 18263723 10.1128/JB.01831-07 1:CAS:528:DC%2BD1cXkslygsLg%3D (Pubitemid 351508202)
9. N Glansdorff Y Xu 2007 Microbial arginine biosynthesis: pathway, regulation and industrial production Microbiol Monogr 5 219 257 10.1007/7171-2006-061 10.1007/7171-2006-061
10. MA Grillo S Colombatto 2004 Arginine revisited: minireview article Amino Acids 26 4 345 351 10.1007/s00726-004-0081-9 15290340 10.1007/s00726-004-0081-9 1:CAS:528:DC%2BD2cXmtVWktLg%3D (Pubitemid 39093137)
11. D Haas V Kurer T Leisinger 1972 N-acetylglutamate synthetase of Pseudomonas aeruginosa. An assay in vitro and feedback inhibition by arginine Eur J Biochem 31 2 290 295 10.1111/j.1432-1033.1972.tb02531.x 4630504 10.1111/j.1432-1033.1972.tb02531.x 1:CAS:528:DyaE3sXmvVKksg%3D%3D
12. T Hermann 2003 Industrial production of amino acids by coryneform bacteria J Biotechnol 104 1-3 155 172 10.1016/s0168-1656(03)00149-4 12948636 10.1016/S0168-1656(03)00149-4 1:CAS:528:DC%2BD3sXmslens7Y%3D (Pubitemid 37011355)
13. J Holatko V Elisakova M Prouza M Sobotka J Nesvera M Patek 2009 Metabolic engineering of the l-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation J Biotechnol 139 3 203 210 10.1016/j.jbiotec.2008.12.005 19121344 10.1016/j.jbiotec.2008.12.005 1:CAS:528:DC%2BD1MXhtlSgtL4%3D
14. M Ikeda 2003 Amino acid production processes Adv Biochem Eng Biotechnol 79 1 35 10.1007/3-540-45989-8-1 12523387 1:CAS:528:DC%2BD3sXhtlSit7g%3D
15. M Ikeda S Mitsuhashi K Tanaka M Hayashi 2009 Reengineering of a Corynebacterium glutamicum l-arginine and l-citrulline producer Appl Environ Microbiol 75 6 1635 1641 10.1128/aem.02027-08 19139237 10.1128/AEM.02027-08 1:CAS:528:DC%2BD1MXjsFKgsb4%3D
16. J Kalinowski B Bathe D Bartels N Bischoff M Bott A Burkovski N Dusch L Eggeling BJ Eikmanns L Gaigalat A Goesmann M Hartmann K Huthmacher R Kramer B Linke AC McHardy F Meyer B Mockel W Pfefferle A Puhler DA Rey C Ruckert O Rupp H Sahm VF Wendisch I Wiegrabe A Tauch 2003 The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins J Biotechnol 104 1-3 5 25 12948626 10.1016/S0168-1656(03)00154-8 1:CAS:528:DC%2BD3sXmslensrY%3D (Pubitemid 37011345)
17. C-D Lu 2006 Pathways and regulation of bacterial arginine metabolism and perspectives for obtaining arginine overproducing strains Appl Microbiol Biot 70 3 261 272 10.1007/s00253-005-0308-z 10.1007/s00253-005-0308-z 1:CAS:528:DC%2BD28XitV2qtrc%3D (Pubitemid 43345150)
18. J Park S Lee 2008 Towards systems metabolic engineering of microorganisms for amino acid production Curr Opin Biotechnol 19 5 454 460 10.1016/j.copbio.2008.08.007 18760356 10.1016/j.copbio.2008.08.007 1:CAS:528:DC%2BD1cXht1SntL7L
19. S Ramón-Maiques ML Fernández-Murga F Gil-Ortiz A Vagin I Fita V Rubio 2006 Structural bases of feed-back control of arginine biosynthesis, revealed by the structures of two hexameric N-acetylglutamate kinases, from Thermotoga maritima and Pseudomonas aeruginosa J Mol Biol 356 3 695 713 10.1016/j.jmb.2005.11.079 16376937 10.1016/j.jmb.2005.11.079 (Pubitemid 43139330)
20. V Sakanyan P Petrosyan M Lecocq A Boyen C Legrain M Demarez JN Hallet N Glansdorff 1996 Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway Microbiology 142 Pt 1 99 108 10.1099/13500872-142-1-99 8581175 10.1099/13500872-142-1-99 1:CAS:528:DyaK28XlsFOmtA%3D%3D (Pubitemid 26032051)
21. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
22. L Sun X Wen Y Tan H Li X Yang Y Zhao B Wang Q Cao C Niu Z Xi 2009 Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase Amino Acids 37 3 523 530 10.1007/s00726-009-0256-5 19266155 10.1007/s00726-009-0256-5 1:CAS:528:DC%2BD1MXhtVaru73P
23. A Tauch O Kirchner B Loffler S Gotker A Puhler J Kalinowski 2002 Efficient electrotransformation of Corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1 Curr Microbiol 45 5 362 367 10.1007/s00284-002-3728-3 12232668 10.1007/s00284-002- 3728-3 1:CAS:528:DC%2BD38XntFentrk%3D (Pubitemid 36935585)
24. T Utagawa 2004 Production of arginine by fermentation J Nutr 134 2854S 2867S 15465800 1:CAS:528:DC%2BD2cXosVOjsLo%3D
25. Wendisch VF, Glansdorff N, Xu Y (2007) Microbial arginine biosynthesis: pathway, regulation and industrial production. Microbiol Monogr 5:219-257. doi: 10.1007/7171-2006-061
26. VF Wendisch 2006 Genetic regulation of Corynebacterium glutamicum metabolism J Microbiol Biotech 16 7 999 1009 1:CAS:528:DC%2BD28XptlShtrc%3D (Pubitemid 44193277)
27. H Xu WF Dou HY Xu XM Zhang ZM Rao ZP Shi ZH Xu 2009 A two-stage oxygen supply strategy for enhanced l-arginine production by Corynebacterium crenatum based on metabolic fluxes analysis Biochem Eng J 43 1 41 51 10.1016/j.bej.2008. 08.007 10.1016/j.bej.2008.08.007 1:CAS:528:DC%2BD1cXhsVers7%2FF
28. MJ Xu ZM Rao H Xu CY Lan WF Dou XM Zhang HY Xu JA Jin ZH Xu 2011 Enhanced production of l-arginine by expression of Vitreoscilla hemoglobin using a novel expression system in Corynebacterium crenatum Appl Biochem Biotechnol 163 6 707 719 10.1007/s12010-010-9076-z 20835781 10.1007/s12010-010-9076-z 1:CAS:528:DC%2BC3MXisFylurs%3D