Inhibitory effect of phospholipids on P-glycoprotein: Cellular studies in Caco-2, MDCKII mdr1 and MDCKII wildtype cells and P-gp ATPase activity measurements

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1821, Issue 9, 2012, Pages 1211-1223

  Simon, S ^a   Schubert, R ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

ATPase activity; Caco 2; Calcein accumulation assay; MDCKII; P glycoprotein inhibition by phospholipid; Transport study

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CALCEIN; CARBON; DOCOSAHEXAENOIC ACID; FATTY ACID; GLYCOPROTEIN P; MONOMER; PHOSPHATIDYLCHOLINE; PHOSPHOLIPID;

ABSORPTION; ANIMAL CELL; ARTICLE; CELL STRAIN; CELL STRAIN CACO 2; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; IN VITRO STUDY; INTESTINE EPITHELIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING;

ADENOSINE TRIPHOSPHATASES; ANIMALS; BIOLOGICAL TRANSPORT; CACO-2 CELLS; DOGS; FATTY ACIDS, UNSATURATED; HUMANS; INTESTINAL MUCOSA; P-GLYCOPROTEIN; PHOSPHATIDYLCHOLINES;

EID: 84862700944     PISSN: 13881981     EISSN: 18792618     Source Type: Journal    
DOI: 10.1016/j.bbalip.2012.06.001     Document Type: Article

References (36)

1. B.I. Florea, I.C. van der Sandt, S.M. Schrier, K. Kooiman, K. Deryckere, A.G. de Boer, H.E. Junginger, and G. Borchard Evidence of P-glycoprotein mediated apical to basolateral transport of flunisolide in human broncho-tracheal epithelial cells (Calu-3) Br. J. Pharmacol. 134 2001 1555 1563
2. F.J. Sharom Shedding light on drug transport: structure and function of the P-glycoprotein multidrug transporter (ABCB1) Biochem. Cell Biol. 84 2006 979 992
3. X. Li-Blatter, P. Nervi, and A. Seelig Detergents as intrinsic P-glycoprotein substrates and inhibitors Biochim. Biophys. Acta 1788 2009 2335 2344
4. A. Seelig, and G. Gerebtzoff Enhancement of drug absorption by noncharged detergents through membrane and P-glycoprotein binding Expert Opin. Drug Metab. Toxicol. 2 2006 733 752
5. C. Martin, G. Berridge, C.F. Higgins, P. Mistry, P. Charlton, and R. Callaghan Communication between multiple drug binding sites on P-glycoprotein Mol. Pharmacol. 58 2000 624 632
6. E.V. Batrakova, S. Li, Y. Li, V.Y. Alakhov, and A.V. Kabanov Effect of pluronic P85 on ATPase activity of drug efflux transporters Pharm. Res. 21 2004 2226 2233
7. B.D. Rege, J.P. Kao, and J.E. Polli Effects of nonionic surfactants on membrane transporters in Caco-2 cell monolayers Eur. J. Pharm. Sci. 16 2002 237 246
8. M.M. Nerurkar, P.S. Burton, and R.T. Borchardt The use of surfactants to enhance the permeability of peptides through Caco-2 cells by inhibition of an apically polarized efflux system Pharm. Res. 13 1996 528 534
9. Y.Y. Chiu, K. Higaki, B.L. Neudeck, J.L. Barnett, L.S. Welage, and G.L. Amidon Human jejunal permeability of cyclosporin A: influence of surfactants on P-glycoprotein efflux in Caco-2 cells Pharm. Res. 20 2003 749 756
10. M.F. Wempe, C. Wright, J.L. Little, J.W. Lightner, S.E. Large, G.B. Caflisch, C.M. Buchanan, P.J. Rice, V.J. Wacher, K.M. Ruble, and K.J. Edgar Inhibiting efflux with novel non-ionic surfactants: rational design based on vitamin E TPGS Int. J. Pharm. 370 2009 93 102
11. Y. Shono, H. Nishihara, Y. Matsuda, S. Furukawa, N. Okada, T. Fujita, and A. Yamamoto Modulation of intestinal P-glycoprotein function by cremophor EL and other surfactants by an in vitro diffusion chamber method using the isolated rat intestinal membranes J. Pharm. Sci. 93 2004 877 885
12. A. Alkatout, Thesis, Department of Pharmaceutical Technology and Biopharmacy, Albert Ludwig University, Freiburg 2008.
13. Y.L. Lo Phospholipids as multidrug resistance modulators of the transport of epirubicin in human intestinal epithelial Caco-2 cell layers and everted gut sacs of rats Biochem. Pharmacol. 60 2000 1381 1390
14. Z. Wang, E. Klipfell, B.J. Bennett, R. Koeth, B.S. Levison, B. Dugar, A.E. Feldstein, E.B. Britt, X. Fu, Y.M. Chung, Y. Wu, P. Schauer, J.D. Smith, H. Allayee, W.H. Tang, J.A. DiDonato, A.J. Lusis, and S.L. Hazen Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease Nature 472 2011 57 63
15. I.J. Hidalgo, T.J. Raub, and R.T. Borchardt Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability Gastroenterology 96 1989 736 749
16. J. de Souza, L.Z. Benet, Y. Huang, and S. Storpirtis Comparison of bidirectional lamivudine and zidovudine transport using MDCK, MDCK-MDR1, and Caco-2 cell monolayers J. Pharm. Sci. 98 2009 4413 4419
17. J. Rautio, J.E. Humphreys, L.O. Webster, A. Balakrishnan, J.P. Keogh, J.R. Kunta, C.J. Serabjit-Singh, and J.W. Polli In vitro p-glycoprotein inhibition assays for assessment of clinical drug interaction potential of new drug candidates: a recommendation for probe substrates Drug Metab. Dispos. 34 2006 786 792
18. G.R. Bartlett Phosphorus assay in column chromatography J. Biol. Chem. 234 1959 466 468
19. M. Holzer, S. Barnert, J. Momm, and R. Schubert Preparative size exclusion chromatography combined with detergent removal as a versatile tool to prepare unilamellar and spherical liposomes of highly uniform size distribution J. Chromatogr. A 1216 2009 5838 5848
20. A. Rank, S. Hauschild, S. Forster, and R. Schubert Preparation of monodisperse block copolymer vesicles via a thermotropic cylinder-vesicle transition Langmuir 25 2009 1337 1344
21. A. Koggel, Thesis, Department of Biopharmacy and Pharmaceutical Technology; Johannes Gutenberg-University, Mainz, 2003.
22. F.J. Sharom, X. Yu, J.W. Chu, and C.A. Doige Characterization of the ATPase activity of P-glycoprotein from multidrug-resistant Chinese hamster ovary cells Biochem. J. 308 Pt 2 1995 381 390
23. B. Biosciences, BD Gentest™ ATPase Assay Kit Data Sheet, 2010.
24. F. Ingels, S. Deferme, E. Destexhe, M. Oth, G. Van den Mooter, and P. Augustijns Simulated intestinal fluid as transport medium in the Caco-2 cell culture model Int. J. Pharm. 232 2002 183 192
25. A.F. Hofmann The continuing importance of bile acids in liver and intestinal disease Arch. Intern. Med. 159 1999 2647 2658
26. S. Lowes, M.E. Cavet, and N.L. Simmons Evidence for a non-MDR1 component in digoxin secretion by human intestinal Caco-2 epithelial layers Eur. J. Pharmacol. 458 2003 49 56
27. I. Avanti(R) polar lipids, critical micelle concentrations (CMCs) p. http://www.avantilipids.com/index.php?option=com-content&view= article&id=1703&Itemid=1422 1999
28. H.M. Yao, and W.L. Chiou The complexity of intestinal absorption and exsorption of digoxin in rats Int. J. Pharm. 322 2006 79 86
29. S.L. Abrahamse, and G. Rechkemmer Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A Pflugers Arch. 441 2001 529 537
30. E.V. Batrakova, S. Li, S.V. Vinogradov, V.Y. Alakhov, D.W. Miller, and A.V. Kabanov Mechanism of pluronic effect on P-glycoprotein efflux system in blood-brain barrier: contributions of energy depletion and membrane fluidization J. Pharmacol. Exp. Ther. 299 2001 483 493
31. E.M. Collnot, C. Baldes, U.F. Schaefer, K.J. Edgar, M.F. Wempe, and C.M. Lehr Vitamin E TPGS P-glycoprotein inhibition mechanism: influence on conformational flexibility, intracellular ATP levels, and role of time and site of access Mol. Pharm. 7 2010 642 651
32. X. Li-Blatter, and A. Seelig Exploring the P-glycoprotein binding cavity with polyoxyethylene alkyl ethers Biophys. J. 99 2010 3589 3598
33. N. Maki, P. Hafkemeyer, and S. Dey Allosteric modulation of human P-glycoprotein. Inhibition of transport by preventing substrate translocation and dissociation J Biol Chem 278 2003 18132 18139
34. I. Avanti(R) Polar Lipids 2011
35. G. Cevc Phospholipid Handbook 1993 Marcel Decker (ed.), Inc. New York
36. M. Saunders Schematic of a Transwell® insert showing the separation of apical and basolateral chambers by a permeable membrane with a confluent cell monolayer on the apical surface http://media.wiley.com/wires/WNAN/WNAN53/ mfig003.jpg 2009 (Ed.), vol. 655 × 243-28 KB