An electrophoretic approach to screen for glutamine deamidation

Analytical Biochemistry

Volumn 428, Issue 1, 2012, Pages 1-3

  Bae, Narkhyun ^a   Yang, Jae Won ^a   Sitte, Harald ^a   Pollak, Arnold ^a   Marquez, Javier ^b   Lubec, Gert ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF MÁLAGA   (Spain)

Author keywords

BN PAGE; Glutamine deamidation; SDS PAGE; Transglutaminase; V8 in gel digestion

Indexed keywords

AMINO ACIDS; CHEMICAL REACTIONS; DIAGNOSIS; DIFFUSERS (OPTICAL); ELECTROPHORESIS; RECOMBINANT PROTEINS; SULFUR COMPOUNDS;

BN-PAGE; DEAMIDATION; INGEL DIGESTION; SDS PAGE; TRANSGLUTAMINASES;

SODIUM DODECYL SULFATE;

GLUTAMINE;

ANIMAL CELL; ARTICLE; BLUE NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; CONTROLLED STUDY; DEAMINATION; LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; RAT; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

RATTUS;

EID: 84862676614     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.05.016     Document Type: Article

References (21)

1. H.T. Wright Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins Crit. Rev. Biochem. Mol. Biol. 26 1991 1 52
2. H. Wang, K.I. Piatkov, C.S. Brower, and A. Varshavsky Glutamine-specific N-terminal amidase, a component of the N-end rule pathway Mol. Cell 34 2009 686 695
3. H. Iwasa, S. Meshitsuka, K. Hongo, T. Mizobata, and Y. Kawata Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR: insight into intrinsically disordered proteins J. Biol. Chem. 286 2011 21796 21805
4. R. Pepperkok, A. Hotz-Wagenblatt, N. Konig, A. Girod, D. Bossemeyer, and V. Kinzel Intracellular distribution of mammalian protein kinase: a catalytic subunit altered by conserved Asn2 deamidation J. Cell Biol. 148 2000 715 726
5. J. Cui, Q. Yao, S. Li, X. Ding, Q. Lu, H. Mao, L. Liu, N. Zheng, S. Chen, and F. Shao Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family Science 329 2010 1215 1218
6. R.J. Truscott Macromolecular deterioration as the ultimate constraint on human lifespan Ageing Res. Rev. 10 2011 397 403
7. X. Li, C. Lin, and P.B. O'Connor Glutamine deamidation: differentiation of glutamic acid and γ-glutamic acid in peptides by electron capture dissociation Anal. Chem. 82 2010 3606 3615
8. H. Liu, G. Gaza-Bulseco, and C. Chumsae Glutamine deamidation of a recombinant monoclonal antibody Rapid Commun. Mass Spectrom. 22 2008 4081 4088
9. N. Bae, A. Pollak, and G. Lubec Proteins from Erwinia asparaginase Erwinase and E. coli asparaginase 2 MEDAC for treatment of human leukemia show a multitude of modifications for which the consequences are completely unclear Electrophoresis 32 2011 1824 1828
10. X. Chao, T.J. Muff, S.Y. Park, S. Zhang, A.M. Pollard, G.W. Ordal, A.M. Bilwes, and B.R. Crane A receptor-modifying deamidase in complex with a signaling phosphatase reveals reciprocal regulation Cell 124 2006 561 571 (Pubitemid 43199441)
11. S. Capasso, L. Mazzarella, and A. Zagari Deamidation via cyclic imide of asparaginyl peptides: dependence on salts, buffers, and organic solvents Pept. Res. 4 1991 234 238
12. M.J. Mycek, and H. Waelsch The enzymatic deamidation of proteins J. Biol. Chem. 235 1960 3513 3517
13. D.M. Hartley, C. Zhao, A.C. Speier, G.A. Woodard, S. Li, Z. Li, and T. Walz Transglutaminase induces protofibril-like amyloid β-protein assemblies that are protease-resistant and inhibit long-term potentiation J. Biol. Chem. 283 2008 16790 16800
14. A.W. Schmid, E. Condemi, G. Tuchscherer, D. Chiappe, M. Mutter, H. Vogel, M. Moniatte, and Y.O. Tsybin Tissue transglutaminase-mediated glutamine deamidation of β-amyloid peptide increases peptide solubility, whereas enzymatic cross-linking and peptide fragmentation may serve as molecular triggers for rapid peptide aggregation J. Biol. Chem. 286 2011 12172 12188
15. J. Stamnaes, B. Fleckenstein, and L.M. Sollid The propensity for deamidation and transamidation of peptides by transglutaminase 2 is dependent on substrate affinity and reaction conditions Biochim. Biophys. Acta 1784 2008 1804 1811
16. D.W. Cleveland, S.G. Fischer, M.W. Kirschner, and U.K. Laemmli Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis J. Biol. Chem. 252 1977 1102 1106 (Pubitemid 8043094)
17. A receptor subunits representing strongly hydrophobic transmembrane proteins J. Proteome Res. 7 2008 3498 3506
18. L. Huang, J. Lu, V.J. Wroblewski, J.M. Beals, and R.M. Riggin In vivo deamidation characterization of monoclonal antibody by LC/MS/MS Anal. Chem. 77 2005 1432 1439 (Pubitemid 40327884)
19. Y.H. Yong, S. Yamaguchi, Y.S. Gu, T. Mori, and Y. Matsumura Effects of enzymatic deamidation by protein-glutaminase on structure and functional properties of α-zein J. Agric. Food Chem. 52 2004 7094 7100
20. M. Ghafari, H. Höger, S. Keihan Falsafi, N. Russo-Schlaff, A. Pollak, and G. Lubec Mass spectrometrical identification of hippocampal NMDA receptor subunits NR1, NR2A-D, and five novel phosphorylation sites on NR2A and NR2B J. Proteome Res. 11 2012 1891 1896
21. M.F. Mazzeo, B. De Giulio, S. Senger, M. Rossi, A. Malorni, and R.A. Siciliano Identification of transglutaminase-mediated deamidation sites in a recombinant α-gliadin by advanced mass-spectrometric methodologies Protein Sci. 12 2003 2434 2442 (Pubitemid 37310785)