Gel-based mass spectrometric analysis of recombinant GABAA receptor subunits representing strongly hydrophobic transmembrane proteins

Journal of Proteome Research

Volumn 7, Issue 8, 2008, Pages 3498-3506

  Kang, Sung Ung ^a,b   Fuchs, Karoline ^b   Sieghart, Werner ^b   Lubec, Gert ^a,c  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b Center for Brain Research   (Austria)

^c UNIV KLIN KINDER JUGENDHEILKUNDE   (Austria)

Author keywords

BN PAGE; BN SDS PAGE; BN SDS SDS PAGE; GABAA receptor; Mass spectrometry; Multienzyme; Transmembrane domain

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; MEMBRANE PROTEIN; PROTEIN SUBUNIT; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BACULOVIRUS; BRAIN TISSUE; CONTROLLED STUDY; HYDROPHOBICITY; INSECT CELL; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RAT; TWO DIMENSIONAL GEL ELECTROPHORESIS; ANIMAL; CELL LINE; CYTOLOGY; ELECTROPHORESIS; ELECTROSPRAY MASS SPECTROMETRY; GEL; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSECT; ISOLATION AND PURIFICATION; RADIOASSAY; TANDEM MASS SPECTROMETRY;

HEXAPODA; MAMMALIA;

ANIMALS; BACULOVIRIDAE; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS; GELS; HYDROPHOBICITY; INSECTS; MEMBRANE PROTEINS; PROTEIN SUBUNITS; RADIOLIGAND ASSAY; RECEPTORS, GABA-A; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 53049091572     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr800236u     Document Type: Article

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