Thermo-responsive non-woven scaffolds for "smart" 3D cell culture

Biotechnology and Bioengineering

Volumn 109, Issue 8, 2012, Pages 2147-2158

  Rossouw, Claire L ^a,b   Chetty, Avashnee ^a   Moolman, Francis Sean ^a   Birkholtz, Lyn Marie ^b   Hoppe, Heinrich ^b,c   Mancama, Dalu T ^a  

^a DSI CSIR Nanotechnology Innovation Centre   (South Africa)

^b UNIVERSITY OF PRETORIA   (South Africa)

^c RHODES UNIVERSITY   (South Africa)

Author keywords

Cell proliferation; Hepatocyte; Non woven fabric; Poly(N isopropylacrylamide); Poly(propylene)

Indexed keywords

3-D CELL CULTURE; ADHERENT CELLS; ALGINATE SCAFFOLDS; CYTOCHROME P450 GENES; DNA QUANTIFICATION; HEPATOCYTES; HOECHST 33258; IN-VITRO; N-ISOPROPYLACRYLAMIDES; NON DESTRUCTIVE; NON-WOVEN; PNIPAAM; SCAFFOLD DEGRADATION; THERMAL RELEASE; THERMO-RESPONSIVE; THERMORESPONSIVE POLYMER; TWO-DIMENSIONAL SURFACE;

ACRYLIC MONOMERS; BIOMATERIALS; CELL CULTURE; CELL ENGINEERING; CELL PROLIFERATION; CELLS; GENE EXPRESSION; GRAFTING (CHEMICAL); MONOLAYERS; POLYETHYLENE TEREPHTHALATES; THREE DIMENSIONAL; TWO DIMENSIONAL; WEAVING;

SCAFFOLDS (BIOLOGY);

ALBUMIN; ALGINIC ACID; CYTOCHROME P450; DNA; MESSENGER RNA; MOLECULAR SCAFFOLD; NYLON; POLY(N ISOPROPYLACRYLAMIDE); POLYETHYLENE TEREPHTHALATE; POLYPROPYLENE;

ARTICLE; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; CELL STRAIN HEPG2; CELL VIABILITY; CONFORMATION; CONTROLLED STUDY; CYTOCHROME P450 GENE; DNA DETERMINATION; EXTRACELLULAR MATRIX; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; HUMAN; HUMAN CELL; LIVER CELL; MONOLAYER CULTURE; PROTEIN SYNTHESIS; TEMPERATURE SENSITIVITY;

ALBUMINS; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CYTOCHROME P-450 ENZYME SYSTEM; HEPATOCYTES; HUMANS; POLYMERS; STAINING AND LABELING; TEMPERATURE; TISSUE SCAFFOLDS;

EID: 84862669717     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24469     Document Type: Article

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