Expression of recombinant Ara h 6 in Pichia pastoris but not in Escherichia coli preserves allergic effector function and allows assessment of specific mutations

Molecular Nutrition and Food Research

Volumn 56, Issue 6, 2012, Pages 986-995

  Zhuang, Yonghua ^a   Durrani, Sandy ^a   D M Hodges, Brittany ^b   Dreskin, Stephen C ^a   Chen, Xueni ^a  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Ara h 6; Basophils; Peanut allergy; Recombinant allergen

Indexed keywords

ARA H 6 ALLERGEN, ARACHIS HYPOGAEA; EPITOPE; IMMUNOGLOBULIN E; MUTANT PROTEIN; NAPIN; PLANT ANTIGEN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BASOPHIL; BASOPHIL DEGRANULATION TEST; BIOSYNTHESIS; BLOOD; CELL CLONE; CELL LINE; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; PEANUT ALLERGY; PICHIA; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN FOLDING; RAT;

2S ALBUMINS, PLANT; ANIMALS; ANTIGENS, PLANT; BASOPHIL DEGRANULATION TEST; BASOPHILS; CELL LINE; CIRCULAR DICHROISM; CLONE CELLS; EPITOPES; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN E; MUTANT PROTEINS; PEANUT HYPERSENSITIVITY; PICHIA; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN FOLDING; RATS; RECOMBINANT PROTEINS;

EID: 84862544670     PISSN: 16134125     EISSN: 16134133     Source Type: Journal    
DOI: 10.1002/mnfr.201100827     Document Type: Article

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