Charge heterogeneity of monoclonal antibodies by multiplexed imaged capillary isoelectric focusing immunoassay with chemiluminescence detection

Analytical Chemistry

Volumn 84, Issue 12, 2012, Pages 5380-5386

  Michels, David A ^a   Tu, Andrea W ^b   McElroy, Will ^a   Voehringer, David ^b   Salas Solano, Oscar ^c  

^a GENENTECH INC   (United States)

^b ProteinSimple   (United States)

^c SEATTLE GENETICS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORBANCE DETECTION; ALTERNATIVE APPROACH; ANALYTICAL METHOD; CAPILLARY ISOELECTRIC FOCUSING; CELLULAR EXTRACTS; CHARGE HETEROGENEITY; CHEMILUMINESCENCE DETECTION; CLONE SELECTION; COATED CAPILLARIES; DETECTION SENSITIVITY; DYNAMIC RANGE; HIGH-THROUGHPUT; HORSE-RADISH PEROXIDASE; ISO-ELECTRIC POINTS; ISOELECTRIC; PROCESS DEVELOPMENT; PRODUCT CHARACTERIZATIONS; REACTIVE SUBSTRATES; RECOMBINANT MONOCLONAL ANTIBODY; SECONDARY ANTIBODIES; WHOLE COLUMN IMAGING DETECTION;

CHEMICAL REACTIONS; CHEMILUMINESCENCE; IMMUNOLOGY; MONOCLONAL ANTIBODIES; MULTIPLEXING; SURFACE PLASMON RESONANCE;

ELECTROPHORESIS;

HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY;

ANIMAL; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; ELECTRON; HAMSTER; IMMUNOASSAY; IMMUNOLOGY; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; LIMIT OF DETECTION; LUMINESCENCE; METABOLISM; METHODOLOGY; PH; REPRODUCIBILITY; STATISTICAL MODEL;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; ELECTRONS; HORSERADISH PEROXIDASE; HYDROGEN-ION CONCENTRATION; IMMUNOASSAY; ISOELECTRIC FOCUSING; LIMIT OF DETECTION; LINEAR MODELS; LUMINESCENT MEASUREMENTS; REPRODUCIBILITY OF RESULTS;

EID: 84862530792     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac3008847     Document Type: Article

References (21)

1. Khawli, L. A.; Goswami, S.; Hutchinson, R.; Kwong, Z. W.; Yang, J.; Wang, X.; Yao, Z.; Sreedhara, A.; Cano, T.; Tesar, D.; Nijem, I.; Allison, D. E.; Wong, P. Y.; Kao, Y.-H.; Quan, C.; Joshi, A.; Harris, R. J.; Motchnik, P. mAbs 2010, 2, 613-624
2. Fang, X.; Tragas, C.; Wu, J.; Mao, Q.; Pawliszyn, J. Electrophoresis 1998, 19, 2290-2295
3. Shimura, K. Electrophoresis 2002, 23, 3847-3857
4. Meert, C. D.; Brady, L. J.; Guo, A.; Balland, A. Anal. Chem. 2010, 82, 3510-3518
5. Zhang, J.; Yip, H.; Katta, V. Anal. Biochem. 2011, 410, 234-243
6. Sosic, Z.; Houde, D.; Blum, A.; Carlage, T.; Lyubarskaya, Y. Electrophoresis 2008, 29, 4368-4376
7. O'Neill, R. A.; Bhamidipati, A.; Bi, X.; Deb-Basu, D.; Cahill, L.; Ferrante, J.; Gentalen, E.; Glazer, M.; Gossett, J.; Hacker, K.; Kirby, C.; Knittle, J.; Loder, R.; Mastroieni, C.; MacLaren, M.; Mills, T.; Nguyen, U.; Parker, N.; Rice, A.; Roach, D.; Suich, D.; Voehringer, D.; Voss, K.; Yang, J.; Yang, T.; Horn, P. B. V. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 16153-16158
8. Knittle, J. E.; Roach, D.; Horn, P. B. V.; Voss, K. O. Anal. Chem. 2007, 79, 9478-9483
9. Dorman, G.; Prestwich, G. D. Trends Biotechnol. 2000, 18, 64-77
10. Janecki, D. J.; Broshears, W. C.; Reilly, J. P. Anal. Chem. 2004, 76, 6643-6650
11. Krawitz, D. C.; Forrest, W.; Moreno, G. T.; Kittleson, J.; Champion, K. M. Proteomics 2006, 6, 94-110
12. Harris, R. J. J. Chromatogr. A 1995, 705, 129-134
13. Wu, X.-Z.; Pawliszyn, J. Electrophoresis 2002, 23, 542-549
14. Wu, J.; Li, S.-C.; Watson, A. J. Chromatogr. A 1998, 817, 163-171
15. Nakane, P. K.; Kawaoi, A. J. Histochem. Cytochem. 1974, 22, 1084-1091
16. Hunt, G.; Hotaling, T.; Chen, A. B. J. Chromatogr. A 1998, 800, 355-367
17. Hunt, G.; Moorhouse, K. G.; Chen, A. B. J. Chromatogr. A 1996, 744, 295-301
18. Li, N.; Kessler, K.; Bass, L.; Zenga, D. J. Pharm. Biomed. Anal. 2007, 43, 963-972
19. Ramsay, L. M.; Dickerson, J. A.; Dada, O.; Dovichi, N. J. Anal. Chem. 2009, 81, 1741-1746
20. Ramsay, L. M.; Dickerson, J. A.; Dovichi, N. J. Electrophoresis 2009, 30, 297-302
21. Billinton, N.; Knight, A. W. Anal. Biochem. 2001, 291, 175-197