Degradable gene delivery systems based on Pluronics-modified low-molecular-weight polyethylenimine: Preparation, characterization, intracellular trafficking, and cellular distribution

International Journal of Nanomedicine

Volumn 7, Issue , 2012, Pages 1127-1138

  Fan, Wei ^a,b   Wu, Xin ^a   Ding, Baoyue ^c   Gao, Jing ^d   Cai, Zhen ^a   Zhang, Wei ^a   Yin, Dongfeng ^a   Wang, Xiang ^a   Zhu, Quangang ^a   Liu, Jiyong ^a   Ding, Xueying ^d   Gao, Shen ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

^b No 425 Hospital of Chinese PLA   (China)

^c JIAXING UNIVERSITY   (China)

^d SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Cellular uptake; Gene transfer; Nonviral vectors; Pluronics; Transfection efficiency

Indexed keywords

COPOLYMER; DEOXYRIBONUCLEASE I; ETHYLENE OXIDE; POLAXAMER MODIFIED POLYETHYLENIMINE; POLOXAMER; POLYETHYLENEIMINE; PROPYLENE OXIDE; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE;

ANALYTICAL PARAMETERS; ARTICLE; CELL MIGRATION; CELL TRANSPORT; CELL VIABILITY; CELLULAR DISTRIBUTION; CHEMICAL ANALYSIS; CHEMICAL MODIFICATION; CHEMICAL PARAMETERS; COMPLEX FORMATION; CYTOTOXICITY; DNA COMPACTION; ENDOCYTOSIS; ENZYMATIC DEGRADATION; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HYDROPHILIC HYDROPHOBIC BALANCE; IN VITRO STUDY; MOLECULAR WEIGHT; NONVIRAL GENE DELIVERY SYSTEM; PARTICLE SIZE; PH; STRUCTURE ACTIVITY RELATION; SURFACE CHARGE; ZETA POTENTIAL; ANALYSIS OF VARIANCE; CELL SURVIVAL; CHEMISTRY; DRUG EFFECT; DRUG STABILITY; HUMAN; METHODOLOGY; PLASMID;

ANALYSIS OF VARIANCE; CELL SURVIVAL; DEOXYRIBONUCLEASES; DRUG STABILITY; HELA CELLS; HUMANS; MOLECULAR WEIGHT; PARTICLE SIZE; PLASMIDS; POLOXAMER; POLYETHYLENEIMINE; TRANSFECTION;

EID: 84862332093     PISSN: 11769114     EISSN: 11782013     Source Type: Journal    
DOI: 10.2147/IJN.S27117     Document Type: Article

References (30)

1. De Smedt SC, Demeester J, Hennink WE. Cationic polymer based gene delivery systems. Pharm Res. 2000;17(2):113-126.
2. Wu X, Ding B, Gao J, et al. Second-generation aptamer-conjugated PSMA-targeted delivery system for prostate cancer therapy. Int J Nanomedicine. 2011;6:1747-1756.
3. Cho KC, Choi SH, Park TG. Low molecular weight PEI conjugated pluronic copolymer: useful additive for enhancing gene transfection efficiency. Macromol Res. 2006;14(3):348-353.
4. Lee K, Bae KH, Lee Y, Lee SH, Ahn CH, Park TG. Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals for magnetically triggered delivery of siRNA. Macromol Biosci. 2010;10(3):239-245.
5. Gebhart CL, Kabanov AV. Evaluation of polyplexes as gene transfer agents. J Control Release. 2001;73(2-3):401-416.
6. Nguyen HK, Lemieux P, Vinogradov SV, et al. Evaluation of polyetherpolyethyleneimine graft copolymers as gene transfer agents. Gene Ther. 2000;7(2):126-138.
7. Lungwitz U, Breunig M, Blunk T, Göpferich A. Polyethyleniminebased non-viral gene delivery systems. Eur J Pharm Biopharm. 2005; 60(2):247-266.
8. Liu T, Yu X, Kan B, et al. Enhanced gene delivery using biodegradable poly(ester amine)s (PEAs) based on low-molecular-weight polyethylenimine and poly(epsilon-caprolactone)-pluronic-poly(epsiloncaprolactone). J Biomed Nanotechnol. 2010;6(4):351-359.
9. Neu M, Fischer D, Kissel T. Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives. J Gene Med. 2005;7(8):992-1009.
10. Yang Z, Sahay G, Sriadibhatla S, Kabanov AV. Amphiphilic block copolymers enhance cellular uptake and nuclear entry of polyplexdelivered DNA. Bioconjug Chem. 2008;19(10):1987-1994.
11. Mintzer MA, Simanek EE. Nonviral vectors for gene delivery. Chem Rev. 2009;109(2):259-302.
12. Guo Q, Shi S, Wang X, et al. Synthesis of a novel biodegradable poly(ester amine) (PEAs) copolymer based on low-molecular-weight polyethyleneimine for gene delivery. Int J Pharm. 2009;379(1):82-89.
13. Ko YT, Kale A, Hartner WC, Papahadjopoulos-Sternberg B, Torchilin VP. Self-assembling micelle-like nanoparticles based on phospholipidpolyethyleneimine conjugates for systemic gene delivery. J Control Release. 2009;133(2):132-138.
14. Kabanov AV, Batrakova EV, Sriadibhatla S, Yang Z, Kelly DL, Alakov VY. Polymer genomics: shifting the gene and drug delivery paradigms. J Control Release. 2005;101(1-3):259-271.
15. Oh KT, Bronich TK, Kabanov AV. Micellar formulations for drug delivery based on mixtures of hydrophobic and hydrophilic Pluronic block copolymers. J Control Release. 2004;94(2-3):411-422.
16. Sahay G, Batrakova EV, Kabanov AV. Different internalization pathways of polymeric micelles and unimers and their effects on vesicular transport. Bioconjug Chem. 2008;19(10):2023-2029.
17. Kabanov AV, Batrakova EV, Alakhov VY. Pluronic block copolymers as novel polymer therapeutics for drug and gene delivery. J Control Release. 2002;82(2-3):189-212.
18. Yin D, Ding X, Gao J, Chu C, Zou H, Gao S. Nonionic amphiphilic surfactant conjuncted polyethyleneimine as a new and highly efficient non-viral gene carrier. Macromol Res. 2009;17(1):19-25.
19. Piest M, Lin C, Mateos-Timoneda MA, et al. Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. J Control Release. 2008; 130(1):38-45.
20. Tang GP, Guo HY, Alexis F, et al. Low molecular weight polyethylenimines linked by beta-cyclodextrin for gene transfer into the nervous system. J Gene Med. 2006;8(6):736-744.
21. Kim TH, Cook SE, Arote RB, et al. A degradable hyperbranched poly(ester amine) based on poloxamer diacrylate and polyethylenimine as a gene carrier. Macromol Biosci. 2007;7(5):611-619.
22. Arote R, Kim TH, Kim YK, et al. A biodegradable poly(ester amine) based on polycaprolactone and polyethylenimine as a gene carrier. Biomaterials. 2007;28(4):735-744.
23. Xu P, Quick GK, Yeo Y. Gene delivery through the use of a hyaluronate-associated intracellularly degradable crosslinked polyethyleneimine. Biomaterials. 2009;30(29):5834-5843.
24. Liang W, Gong H, Yin D, Lu S, Fu Q. High-molecular-weight polyethyleneimine conjuncted pluronic for gene transfer agents. Chem Pharm Bull (Tokyo). 2011;59(9):1094-1101.
25. Xu Z, Shen G, Xia X, et al. Comparisons of three polyethyleneiminederived nanoparticles as a gene therapy delivery system for renal cell carcinoma. J Transl Med. 2011;9(1):46.
26. Berthold PR, Shiraishi T, Nielsen PE. Cellular delivery and antisense effects of peptide nucleic acid conjugated to polyethyleneimine via disulfide linkers. Bioconjug Chem. 2010;21(10):1933-1938.
27. Roques C, Fattal E, Fromes Y. Comparison of toxicity and transfection efficiency of amphiphilic block copolymers and polycationic polymers in striated muscles. J Gene Med. 2009;11(3):240-249.
28. Hu X, Ji J. Covalent layer-by-layer assembly of hyperbranched polyether and polyethyleneimine: multilayer films providing possibilities for surface functionalization and local drug delivery. Biomacromolecules. 2011;12(12):4264-4271. Epub November 10, 2011.
29. Vinogradov SV, Kohli E, Zeman AD. Comparison of nanogel drug carriers and their formulations with nucleoside 5'-triphosphates. Pharm Res. 2006;23(5):920-930.
30. Xia T, Kovochich M, Liong M, et al. Polyethyleneimine coating enhances the cellular uptake of mesoporous silica nanoparticles and allows safe delivery of siRNA and DNA constructs. ACS Nano. 2009; 3(10):3273-3286.