A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

BMC Research Notes

Volumn 5, Issue , 2012, Pages

  Orlova, Nadezhda A ^a   Orlov, Alexandre V ^b   Vorobiev, Ivan I ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b Datanium Jsc   (Russian Federation)

Author keywords

Cloning technique; Hybrid restriction site; Molecular cloning; Software tool

Indexed keywords

CRICETULUS GRISEUS;

ELONGATION FACTOR; RECOMBINANT DNA; RESTRICTION ENDONUCLEASE;

ALGORITHM; ANIMAL; ARTICLE; CHO CELL; COMPUTER PROGRAM; CRICETULUS; DNA SEQUENCE; GENETICS; HAMSTER; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; RNA TRANSLATION;

ALGORITHMS; ANIMALS; BASE SEQUENCE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; DNA RESTRICTION ENZYMES; DNA, RECOMBINANT; MOLECULAR SEQUENCE DATA; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PEPTIDE ELONGATION FACTORS; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SEQUENCE ANALYSIS, DNA; SOFTWARE DESIGN;

MLCS; MLOWN;

EID: 84862260010     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-5-303     Document Type: Article

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