A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture

Protein and Cell

Volumn 3, Issue 1, 2012, Pages 51-59

  Wang, Qihui ^a,b   Mou, Xiaoning ^a,b   Cao, Henghua ^a   Meng, Qingzhang ^a   Ma, Yanni ^a   Han, Pengcheng ^a,b   Jiang, Junjie ^a,b   Zhang, Hao ^c   Ma, Yue ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c FUWAI HOSPITAL   (China)

Author keywords

human embryonic stem cells; human induced pluripotent stem cells; reprogramming; xeno free and feeder cell free culture system

Indexed keywords

OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2; SCLEROPROTEIN; SODIUM CHLORIDE; TRANSCRIPTION FACTOR;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL FREE SYSTEM; CELL RENEWAL; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; EMBRYONIC STEM CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROCESS DEVELOPMENT; CHEMISTRY; CYTOLOGY; FEMALE; FIBROBLAST; GENETICS; ISOLATION AND PURIFICATION; LENTIVIRINAE; METABOLISM; METHODOLOGY; NUCLEAR REPROGRAMMING; PLACENTA; PREGNANCY;

ANIMALIA;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA; EXTRACELLULAR MATRIX PROTEINS; FEMALE; FIBROBLASTS; HUMANS; LENTIVIRUS; NUCLEAR REPROGRAMMING; PLACENTA; PLURIPOTENT STEM CELLS; PREGNANCY; SODIUM CHLORIDE; TRANSCRIPTION FACTORS;

EID: 84862255778     PISSN: 1674800X     EISSN: 16748018     Source Type: Journal    
DOI: 10.1007/s13238-012-2002-0     Document Type: Article

References (25)

1. Aasen, T., Raya, A., Barrero, M. J., Garreta, E., Consiglio, A., Gonzalez, F., Vassena, R., Bilic, J., Pekarik, V., Tiscornia, G., et al. (2008). Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol 26, 1276-1284.
2. Amit, M., Shariki, C., Margulets, V., and Itskovitz-Eldor, J. (2004). Feeder layer-and serum-free culture of human embryonic stem cells. Biol Reprod 70, 837-845.
3. Beattie, G. M., Lopez, A. D., Bucay, N., Hinton, A., Firpo, M. T., King, C. C., and Hayek, A. (2005). Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23, 489-495.
4. Braam, S. R., Zeinstra, L., Litjens, S., Ward-van Oostwaard, D., van den Brink, S., van Laake, L., Lebrin, F., Kats, P., Hochstenbach, R., Passier, R., et al. (2008). Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell selfrenewal via alphavbeta5 integrin. Stem Cells 26, 2257-2265.
5. Draper, J. S., Moore, H. D., Ruban, L. N., Gokhale, P. J., and Andrews, P. W. (2004). Culture and characterization of human embryonic stem cells. Stem Cells Dev 13, 325-336.
6. Ghodsizadeh, A., Taei, A., Totonchi, M., Seifinejad, A., Gourabi, H., Pournasr, B., Aghdami, N., Malekzadeh, R., Almadani, N., Salekdeh, G. H., et al. (2010). Generation of liver disease-specific induced pluripotent stem cells along with efficient differentiation to functional hepatocyte-like cells. Stem Cell Rev 6, 622-632.
7. Hanna, J., Wernig, M., Markoulaki, S., Sun, C. W., Meissner, A., Cassady, J. P., Beard, C., Brambrink, T., Wu, L. C., Townes, T. M., et al. (2007). Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science 318, 1920-1923.
8. Huangfu, D., Osafune, K., Maehr, R., Guo, W., Eijkelenboom, A., Chen, S., Muhlestein, W., and Melton, D. A. (2008). Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol 26, 1269-1275.
9. Kibbey, M. C. (1994). Maintenance of the EHS sarcoma and Matrigel preparation. Methods Cell Sci 16, 227-230.
10. Lee, G., Papapetrou, E. P., Kim, H., Chambers, S. M., Tomishima, M. J., Fasano, C. A., Ganat, Y. M., Menon, J., Shimizu, F., Viale, A., et al. (2009). Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs. Nature 461, 402-406.
11. Li, Z. K., and Zhou, Q. (2010). Cellular models for disease exploring and drug screening. Protein Cell 1, 355-362.
12. Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., Crandall, L. J., Daigh, C. A., Conard, K. R., Piekarczyk, M. S., et al. (2006). Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24, 185-187.
13. Ma, Y., Ramezani, A., Lewis, R., Hawley, R. G., and Thomson, J. A. (2003). High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors. Stem Cells 21, 111-117.
14. Martin, M. J., Muotri, A., Gage, F., and Varki, A. (2005). Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med 11, 228-232.
15. Park, I. H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M. W., Cowan, C., Hochedlinger, K., and Daley, G. Q. (2008). Disease-specific induced pluripotent stem cells. Cell 134, 877-886.
16. Somers, A., Jean, J. C., Sommer, C. A., Omari, A., Ford, C. C., Mills, J. A., Ying, L., Sommer, A. G., Jean, J. M., Smith, B. W., et al. (2010). Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassette. Stem Cells 28, 1728-1740.
17. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872.
18. Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., and Jones, J. M. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147.
19. Wang, P., and Na, J. (2011). Mechanism and methods to induce pluripotency. Protein Cell 2, 792-799.
20. Wernig, M., Zhao, J. P., Pruszak, J., Hedlund, E., Fu, D., Soldner, F., Broccoli, V., Constantine-Paton, M., Isacson, O., and Jaenisch, R. (2008). Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. Proc Natl Acad Sci USA 105, 5856-5861.
21. Xu, C., Inokuma, M. S., Denham, J., Golds, K., Kundu, P., Gold, J. D., and Carpenter, M. K. (2001). Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19, 971-974.
22. Xu, D., Alipio, Z., Fink, L. M., Adcock, D. M., Yang, J., Ward, D. C., and Ma, Y. (2009). Phenotypic correction of murine hemophilia A using an iPS cell-based therapy. Proc Natl Acad Sci USA 106, 808-813.
23. Yamanaka, S. (2008). Pluripotency and nuclear reprogramming. Philos Trans R Soc Lond B Biol Sci 363, 2079-2087.
24. Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920.
25. Zhang, X., and Gao, G. F. (2010). Revival of gene therapy. Protein Cell 1, 107-108.