Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR ® Identifiler ® profiles for genotyping of human DNA

Investigative Genetics

Volumn 3, Issue 1, 2012, Pages

  Foster, Amanda ^a   Laurin, Nancy ^b  

^a CARLETON UNIVERSITY   (Canada)

^b ROYAL CANADIAN MOUNTED POLICE   (Canada)

Author keywords

DNA typing; Forensic science; Identifiler; Rapid PCR; Short tandem repeat

Indexed keywords

DNA; DNA POLYMERASE; TAQ POLYMERASE;

ANALYTICAL EQUIPMENT; ARTICLE; ARTIFACT REDUCTION; CONTROLLED STUDY; CROSS REACTION; DNA DETERMINATION; DNA FINGERPRINTING; FEASIBILITY STUDY; FEMALE; FORENSIC SCIENCE; GENE AMPLIFICATION; GENE LOCUS; GENE TARGETING; GENOTYPE; HETEROZYGOTE; HUMAN; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUALITY CONTROL; RELIABILITY; SHORT TANDEM REPEAT; THERMODYNAMICS; TIME; VALIDATION PROCESS;

EID: 84862211436     PISSN: None     EISSN: 20412223     Source Type: Journal    
DOI: 10.1186/2041-2223-3-6     Document Type: Article

References (20)

1. Laurin N, Frégeau C. Optimization and validation of a fast amplification protocol for AmpFℓSTR® Profiler Plus® for rapid forensic human identification. Forensic Sci Int Genet 2012, 6:47-57. 10.1016/j.fsigen.2011.01.011, 21397581.
2. Verheij S, Harteveld J, Sijen T. A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples. Forensic Sci Int Genet 2011,
3. Giese H, Lam R, Selden R, Tan E. Fast multiplexed polymerase chain reaction for conventional and microfluidic short tandem repeat analysis. J Forensic Sci 2009, 54(6):1287-1296. 10.1111/j.1556-4029.2009.01200.x, 19840207.
4. Wang DY, Change CW, Hennessy LK. Rapid STR analysis of single source DNA samples in 2h. Forensic Sci Int Genet 2009, 2:115-116.
5. Vallone PM, Hill CR, Butler JM. Demonstration of rapid multiplex PCR amplification involving 16 genetic loci. Forensic Sci Int Genet 2008, 3:42-45. 10.1016/j.fsigen.2008.09.005, 19083866.
6. Linacre A, Pekarek V, Swaran YC, Tobe SS. Generation of DNA profiles from fabrics without DNA extraction. Forensic Sci Int Genet 2010, 4:137-141. 10.1016/j.fsigen.2009.07.006, 20129473.
7. Horsman KM, Bienvenue JM, Blasier KR, Landers JP. Forensic DNA analysis on microfluidic devices: a review. J Forensic Sci 2007, 52(4):784-799. 10.1111/j.1556-4029.2007.00468.x, 17553097.
8. Liu P, Yeung SHI, Crenshaw KA, Crouse CA, Scherer JR, Mathies RA. Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer. Forensic Sci Int Genet 2008, 2:301-309. 10.1016/j.fsigen.2008.03.009, 19083840.
9. Bienvenue JM, Legendre LA, Ferrance JP, Landers JP. An integrated microfluidic device for DNA purification and PCR amplification of STR fragments. Forensic Sci Int Genet 2010, 4:178-186. 10.1016/j.fsigen.2009.02.010, 20215029.
10. Frégeau CJ, De Moors A. Competition for DNA binding sites using the Promega DNA IQ™ paramagnetic beads. Forensic Sci Int Genet 2011,
11. Validation Guide for the DNA IQ™ Casework Pro Kit for Maxwell® 16 2010, Reference Manual (Part# GE621), Madison, WI: Promega Corporation.
12. Collins PJ, Hennessy LK, Leibelt CS, Roby RK, Reeder DJ, Foxall PA. Developmental validation of a single-tube amplification of the 13 CODIS STR loci, D2S1338, D19S433, and amelogenin: the AmpFℓSTR® Identifiler® PCR amplification kit. J Forensic Sci 2004, 49(6):1265-1277.
13. AmpFℓSTR® Identifiler® PCR Amplification Kit User's Manual 2011, Foster City, CA: Applied Biosystems.
14. C1000™ thermal cycler Instruction Manual, 10010305 Rev B 2008, Mississauga, ON: Bio-Rad Laboratories Inc.
15. Frégeau CJ, Bowen KL, Fourney RM. Validation of highly polymorphic fluorescent multiplex short tandem repeat systems using two generations of DNA sequencers. J Forensic Sci 1999, 44:133-166.
16. Gibb AJ, Huell AL, Simmons MC, Brown RM. Characterization of forward stutter in the AmpFℓSTR® SGM® PCR. Sci Justice 2009, 49:24-31. 10.1016/j.scijus.2008.05.002, 19418925.
17. Frégeau CJ, Lett M, Fourney RM. Validation of a DNA IQTM-based extraction method for TECAN robotic liquid handling workstations for processing casework. Forensic Sci Int Genet 2010, 4:292-304. 10.1016/j.fsigen.2009.11.001, 20457033.
18. Frégeau CJ, Lett CM, Elliot J, Yensen C, Fourney RM. Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: contamination prevention. J Forensic Sci 2008, 53:632-651. 10.1111/j.1556-4029.2008.00697.x, 18471209.
19. Frégeau CJ, Yensen C, Elliot J, Fourney RM. Optimized configuration of fixed tip robotic liquid-handling stations for the elimination of biological sample cross-contamination. JALA 2007, 12:339-354.
20. Applied Biosystems Quantifiler™ Kits User's Manual 2003, Foster City, CA: Applied Biosystems.