Validation of a DNA IQ™-based extraction method for TECAN robotic liquid handling workstations for processing casework

Forensic Science International: Genetics

Volumn 4, Issue 5, 2010, Pages 292-304

  Frégeau, Chantal J ^a   Lett, C Marc ^a   Fourney, Ron M ^a  

^a ROYAL CANADIAN MOUNTED POLICE   (Canada)

Author keywords

Automation; Casework; DNA IQ; DNA typing; Fixed tips; Magnetic beads

Indexed keywords

CHLOROFORM; PHENOL;

ARTICLE; BLOOD SAMPLING; CLINICAL PROTOCOL; COMPUTER AIDED DESIGN; CONTAMINATION; CONTROLLED STUDY; DNA EXTRACTION; DNA FINGERPRINTING; DNA PROTEIN COMPLEX; DNA PURIFICATION; ELUTION; GENETIC COUNSELING; HUMAN; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; ROBOTICS; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

AUTOMATION; DNA; HUMANS; REPRODUCIBILITY OF RESULTS; ROBOTICS;

EID: 77956901464     PISSN: 18724973     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fsigen.2009.11.001     Document Type: Article

References (41)

1. C.J. Frégeau, B. Leclair, K. Bowen, F. Porelle, and R. Fourney The National DNA Data Bank of Canada - a laboratory bench retrospective on the first year of operation B. Brinkmann, A. Carracedo, Progress in Forensic Genetics 9, Proceedings of the 19th International ISFG Congress August 28-September 1, 2001, Münster, Germany 2003 621 625 Int. Congress Series 1239
2. A. Hopwood, J. Brookes, A. Shariff, P. Cage, E. Tatum, and R. Mirza A fully integrated robotic system for high sample throughput within a DNA databasing unit Proceedings from the Eighth International Symposium on Human Identification September 17-20, 1997 1998 Promega Corporation Orlando (FL) 20 24
3. M. Steinlechner, and W. Parson Automation and high through-put for a DNA database laboratory: development of a laboratory information management system Croat. Med. J. 42 2001 252 255
4. ® 2000 and the DNA IQ™ System Profiles DNA 5 2002 3 5
5. ® 2000 Laboratory Automation workstation and the DNA IQ™ System to the extraction of forensic casework samples J. Forensic Sci. 49 2004 29 39
6. C. Crouse, S. Yeung, S. Greenspoon, A. McGuckian, J. Sikorsky, and J. Ban Improving efficiency of a small forensic DNA laboratory: validation of robotic assays and evaluation of microcapillary array device Croat. Med. J. 46 2005 563 577
7. M. Nagy, P. Otremba, C. Krüger, S. Bergner-Greiner, P. Anders, and B. Henske Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensics Forensic Sci. Int. 152 2005 13 22
8. C.J. Frégeau, M. Lett, J. Elliott, K.L. Bowen, T. White, and R.M. Fourney Adoption of automated DNA processing for high volume DNA casework: a combined approach using magnetic beads and real-time PCR A. Amorim, F. Corte-Real, N. Morling, Progress in Forensic Genetics 11, Proceedings of the 21st International ISFG Congress; September 13-16, 2005 Ponta Delgada, The Azores, Portugal 2006 688 690 Int. Congress Series 1288
9. R. Kishore, W. Reef Hardy, V.J. Anderson, N.A. Sanchez, and M.R. Buoncristiani Optimization of DNA extraction from low-yield and degraded samples using the BioRobot EZ1 and BioRobot M48 J. Forensic Sci. 51 2006 1055 1061
10. L.C. Tack, M. Thomas, and K. Reich Automated forensic DNA purification optimized for FTA card punches and Identifiler STR-based PCR analysis Clin. Lab. Med. 27 2007 183 191
11. B. Leclair, and T. Scholl Application of automation and information systems to forensic genetic specimen processing Expert Rev. Mol. Diagn. 5 2005 241 250
12. DNA IQ™ System-Small sample casework protocol. Technical Bulletin No. 296. Promega Corporation, Inc., June 2002; revised 04/06.
13. C. Carroll, and C. Yensen Comparison of yield and PCR sensitivity of the DNA IQ™ and phenol/chloroform extraction methods Can. Soc. Forensic Sci. J. 36 2003 61
14. ® DNA Extraction System for potential use with mass disaster samples Can. Soc. Forensic Sci. J. 38 2005 151 163
15. R.R. Mondesire, D.L. Kozwich, K.A. Johansen, J.C. Gerdes, and S.E. Beard Solid-phase Nucleic Acid Extraction, Amplification and Detection May/June 2000 IVD Technology
16. S. Beard A Novel Solid-phase Nucleic Acid Extraction Kit for High-throughput Sample Preparation August 2002 American Biotechnology Laboratory
17. M. Taylor, C. Bridge, M. Baker, Improved Sensitivity for Forensic DNA Purification using ChargeSwitch1 Technology, DNA Research Innovations Ltd., 2004 www.invitrogen. com/NAPrep.
18. C.J. Frégeau, C.M. Lett, J. Elliott, C. Yensen, and R.M. Fourney Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: contamination prevention J. Forensic Sci. 53 2008 632 651
19. C.C. Tebbe, and W. Vahjen Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast Appl. Environ. Microbiol. 59 1993 2657 2665
20. D. Sutlović, M. Definis Gojanović,. Anelinović, D. Gugić, and D. Primorac Taq polymerase reverses inhibition of quantitative real time polymerase chain reaction by humic acid Croat. Med. J. 46 2005 556 562
21. C.T. Comey, and B. Budowle Validation studies on the analysis of the HLA DQα locus using the polymerase chain reaction J. Forensic Sci. 36 1991 1633 1648
22. J.M. Wallin, M.R. Buoncristiani, K.D. Lazaruk, N. Fildes, C.L. Holt, and P.S. Walsh TWGDAM validation of the AmpFlSTR™ Blue PCR Amplification kit for forensic casework analysis J. Forensic Sci. 43 1998 854 870
23. C.J. Frégeau, K.L. Bowen, and R.M. Fourney Validation of highly polymorphic fluorescent multiplex short tandem repeat systems using two generations of DNA sequencers J. Forensic Sci. 44 1999 133 166
24. C.J. Frégeau, C. Yensen, J. Elliott, and R.M. Fourney Optimized configuration of fixed-tip robotic liquid handling stations for the elimination of biological sample cross-contamination JALA 12 2007 339 354
25. G. Porter, C.J. Frégeau, Elimination of Amplifiable Nucleic-acid Carryover From Fixed Tips on an Automated Liquid Handler in a Forensic DNA Laboratory - Method Validation Study, TECAN Application Note, August 2007, pp. 1-8.
26. C.J. Frégeau, O. Germain, and R.M. Fourney Fingerprint enhancement revisited and the effects of blood enhancement chemicals on subsequent Profiler Plus™ fluorescent short tandem repeat DNA analysis of fresh and aged bloody fingerprints J. Forensic Sci. 45 2000 354 380
27. Quantifiler™ Kits User's Manual, Applied Biosystems, Version dated 10/2003.
28. S.A. Montpetit, I.T. Fitch, and P.T. O'Donnell A simple automated instrument for DNA extraction in forensic casework J. Forensic Sci. 50 2005 555 563
29. P. Hoff-Olsen, B. Mevg, E. Staalstrøm, B. Hovde, T. Egeland, and B. Olaisen Extraction of DNA from decomposed human tissue - an evaluation of five extraction methods for short tandem repeat typing Forensic Sci. Int. 105 1999 171 183
30. B. Böddinghaus, T.A. Wichelhaus, V. Brade, and T. Bittner Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis Kit J. Clin. Microbiol. 39 2001 3750 3752
31. A.L. McOrist, M. Jackson, and A.R. Bird A comparison of five methods for extraction of bacterial DNA from human faecal samples J. Microbiol. Methods 50 2002 131 139
32. R. Bitner, S.C. Koller, Automated genomic DNA purification options in agricultural applications using MagneSil™ paramagnetic particles, in: D.J. Bornhop et al. (Eds.), Biomedical Nanotechnology Architectures and Applications, Proceedings of SPIE 4626 (2002) 218-225.
33. J.M. Nechvatal, J.L. Ram, M.D. Basson, P. Namprachan, S.R. Niec, K.Z. Badsha, L.H. Matherly, A.P.N. Majumdar, and I. Kato Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces J. Microbiol. Methods 72 2008 124 132
34. M.L. Smit, B.A.J. Giesendorf, S.G. Heil, J.A.M. Vet, F.J.M. Trijbels, and H.J. Blom Automated extraction and amplification of DNA from whole blood using a robotic workstation and an integrated thermocycler Biotechnol. Appl. Biochem. 32 2000 121 125
35. ® 100 Profiles DNA 9 2006 8 10
36. ® 3100 Genetic Analyzer, RCMP Internal Validation Report, April 2007.
37. ® kits QIAGEN News 1 2002 22 23
38. C.C. Young, R.L. Burghoff, L.G. Keim, V. Minak-Bernero, J.R. Lute, and S.M. Hinton Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils Appl. Environ. Microbiol. 59 1993 1972 1974
39. B.P. Chowdhary, and T. Raudsepp The Horse Genome Derby: racing from map to whole genome sequence Chromosome Res. 16 2008 109 127
40. http://www.sanger.ac.uk/Projects/S-scrofa/
41. http://www.ornl.org/sci/techresources/Human-Genome/faq/compgen.shtml