Automated segmentation of comet assay images using Gaussian filtering and fuzzy clustering

Medical and Biological Engineering and Computing

Volumn 50, Issue 5, 2012, Pages 523-532

  Sansone, Mario ^a   Zeni, Olga ^b   Esposito, Giovanni ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b INSTITUTE FOR ELECTROMAGNETIC SENSING OF THE ENVIRONMENT   (Italy)

Author keywords

Comet assay; DNA damageV; Fuzzy clustering; Nucleic acid; Segmentation

Indexed keywords

AUTOMATED SEGMENTATION; BIOMONITORING; COMET ASSAYS; COMMERCIAL SYSTEMS; DNA DAMAGES; DNA DAMAGING AGENTS; FUZZY SEGMENTATION; GAUSSIAN FILTERING; GAUSSIANS; GENOTOXIC EFFECTS; HUMAN LEUKOCYTES; MORPHOLOGICAL OPERATOR; PRE-FILTERING; SINGLE-CELL LEVEL; USER INTERACTION;

ALGORITHMS; DAMAGE DETECTION; DNA; FUZZY CLUSTERING; NUCLEIC ACIDS;

IMAGE SEGMENTATION;

MESYLIC ACID METHYL ESTER; MUTAGENIC AGENT;

ALGORITHM; ARTICLE; COMET ASSAY; DNA DAMAGE; DRUG EFFECT; EVALUATION; FLUORESCENCE MICROSCOPY; FUZZY LOGIC; HUMAN; IMAGE PROCESSING; LEUKOCYTE; METHODOLOGY;

ALGORITHMS; COMET ASSAY; DNA DAMAGE; FUZZY LOGIC; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LEUKOCYTES; METHYL METHANESULFONATE; MICROSCOPY, FLUORESCENCE; MUTAGENS;

EID: 84862171757     PISSN: 01400118     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11517-012-0882-z     Document Type: Article

References (23)

1. Arambula Cosio F, Marquez Flores JA, Padilla Castaneda MA, Solano S, Tato P (2005) Automatic analysis of immunocytochemically stained tissue samples. Med Biol Eng Comput 43(5):672-677 (Pubitemid 41824354)
2. Bezdek JC, Keller J, Krisnapuram R, Pal NR (2005) Fuzzy Models and Algorithms for Pattern Recognition and Image Processing. In: Handbooks of Fuzzy Sets. Springer, New York
3. Bocker W, Rolf W, Bauch T, Mller WU, Streffer C (1999) Automated comet assay analysis. Cytometry 35(2):134-143
4. Collins A, Duinsk M, Franklin M, Somorovsk M, Petrovsk H, Duthie S, Fillion L, Panayiotidis M, Ralov K, Vaughan N (1997) Comet assay in human biomonitoring studies: reliability, validation, and applications. Environ Mol Mutagen 30(2):139-146 (Pubitemid 27440590)
5. Collins AR (2004) The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 26(3):249-261 (Pubitemid 41490465)
6. Collins AR, Dusinsk M, Gedik CM, Stetina R (1996) Oxidative damage to DNA: do we have a reliable biomarkeř. Environ Health Perspect 104(Suppl 3):465-469 (Pubitemid 26197677)
7. Dehon G, Catoire L, Duez P, Bogaerts P, Dubois J (2008) Validation of an automatic comet assay analysis system integrating the curve fitting of combined comet intensity profiles. Mutat Res Genet Toxicol Environ Mutagen 650(2):87-95
8. Duez P, Dehon G, Dubois J (2004) Validation of raw data measurements in the comet assay. Talanta 63(4):879-886
9. Esmaeilsabzali H, Sakaki K, Dechev N, Burke RD, Park EJ (2012) Machine vision-based localization of nucleic and cytoplasmic injection sites on low-contrast adherent cells. Med Biol Eng Comput 50(1):11-21
10. Frieauff W, Hartmann A, Suter W (2001) Automatic analysis of slides processed in the comet assay. Mutagenesis 16(2):133-137 (Pubitemid 32184476)
11. Helma C, Uhl M (2000) A public domain image-Analysis program for the single-cell gel-electrophoresis (comet) assay. Mutat Res 466(1):9-15 (Pubitemid 30177133)
12. Kharma N, Moghnieh H, Yao J, Guo YP, Abu-Baker A, Laganiere J, Rouleau G, Cheriet M (2007) Automatic segmentation of cells from microscopic imagery using ellipse detection. IET Image Process 1(1):39-47 (Pubitemid 46543593)
13. Konca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Gozdz S, Koza Z, Wojcik A (2003) A cross-platform public domain PC image-Analysis program for the comet assay. Mutat Res Genet Toxicol Environ Mutagen 534:15-20 (Pubitemid 36015835)
14. Kumaravel TS, Vilhar B, Faux SP, Jha AN (2007) Comet assay measurements: a perspective. Cell Biol Toxicol 25(1):53-63
15. Lovell DP, Omori T (2008) Statistical issues in the use of the comet assay. Mutagenesis 23(3):171-182
16. Ostling O, Johanson KJ (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123(1):291-298 (Pubitemid 14030137)
17. Rivest J-F, Tang M, McLean J, Johnson F (1996) Automated measurements of tails in the single cell gel electrophoresis assay. In: Instrumentation and Measurement Technology Conference, 1996. IMTC-96. Conference Proceedings. Quality Measurements: The Indispensable Bridge between Theory and Reality, IEEE, vol 1, pp 111-113
18. Serra J (1986) Introduction to mathematical morphology. Comput Vis Gr Image Process 35(3):283-305
19. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175(1):184-191
20. Smolka B, Lukac R (2004) Segmentation of the comet assay images. In: Campilho, AC, Kamel MS (eds) ICIAR (2), lecture notes in computer science, vol 3212. Springer, Berlin, pp 124-131 (Pubitemid 39749706)
21. Tice RR, Agurell E, Anderson D, Burloinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35(3):206-221 (Pubitemid 30196665)
22. Zeni O, Scarfí MR (2010) DNA damage by carbon nanotubes using the single cell gel electrophoresis technique. Methods Mol Biol (Clifton, N.J.) 625:109-119
23. Zheru Chi Hong Yan TP (1996) Fuzzy Algorithms: with applications to image processing and pattern recognition. In: Advances in fuzzy systems. Applications and theory, vol 10. World Scientific Pub Co. Inc., Singapore