The polarized total internal reflection fluorescence microscopy (poLTIRFM) twirling filament assay

Cold Spring Harbor Protocols

Volumn 7, Issue 6, 2012, Pages 719-721

  Beausang, John F ^a   Sun, Yujie ^a   Quinlan, Margot E ^a   Forkey, Joseph N ^a   Goldman, Yale E ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; F ACTIN; FLUORESCENT DYE; MYOSIN V; ACTIN;

ACTIN FILAMENT; ARTICLE; ASSAY; FLUORESCENCE MICROSCOPY; OPTICAL ROTATION; POLARIZED TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; SPATIAL ORIENTATION; THREE DIMENSIONAL IMAGING; TWIRLING FILAMENT ASSAY; MACROMOLECULE; METABOLISM; METHODOLOGY; POLARIZATION MICROSCOPY; SKELETAL MUSCLE; STAINING;

ACTINS; FLUORESCENT DYES; MACROMOLECULAR SUBSTANCES; MICROSCOPY, FLUORESCENCE; MICROSCOPY, POLARIZATION; MUSCLE FIBERS, SKELETAL; MYOSIN TYPE V; STAINING AND LABELING;

EID: 84862134298     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot069401     Document Type: Article

References (5)

1. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012a. Preparation of filamentous actin for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069377.
2. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012b. Construction of flow chambers for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069385.
3. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012c. Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM). Cold Spring Harb Protoc doi: 10.1101/pdb.top069344.
4. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012d. The acquisition and analysis of polarized total internal reflection fluorescence microscopy (polTIRFM) data. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069419.
5. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012e. The polarized total internal reflection fluorescence microscopy (polTIRFM) processive motility assay for myosin V. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069393.