Construction of flow chambers for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays

Cold Spring Harbor Protocols

Volumn 7, Issue 6, 2012, Pages 712-715

  Beausang, John F ^a   Sun, Yujie ^a   Quinlan, Margot E ^a   Forkey, Joseph N ^a   Goldman, Yale E ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; ACTIN; MYOSIN V;

ARTICLE; ASSAY; AUTOFLUORESCENCE; FLUORESCENCE MICROSCOPY; FLUORESCENCE POLARIZATION; OPTICAL ROTATION; POLARIZED TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; PRISM; PROTEIN MOTILITY ASSAY; SPATIAL ORIENTATION; THREE DIMENSIONAL IMAGING; MACROMOLECULE; METABOLISM; METHODOLOGY; POLARIZATION MICROSCOPY; SKELETAL MUSCLE;

ACTINS; FLUORESCENT DYES; MACROMOLECULAR SUBSTANCES; MICROSCOPY, FLUORESCENCE; MICROSCOPY, POLARIZATION; MUSCLE FIBERS, SKELETAL; MYOSIN TYPE V;

EID: 84862127789     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot069385     Document Type: Article

References (4)

1. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012a. The polarized total internal reflection fluorescence microscopy (polTIRFM) processive motility assay for myosin V. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069393.
2. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012b. The polarized total internal reflection fluorescence microscopy (polTIRFM) twirling filament assay. Cold Spring Harb Protoc doi: 10.1101/pdb. prot069401.
3. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012c. Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM). Cold Spring Harb Protoc doi: 10.1101/pdb.top069344.
4. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012d. The acquisition and analysis of polarized total internal reflection fluorescence microscopy (polTIRFM) data. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069419.