The acquisition and analysis of polarized total internal reflection fluorescence microscopy (polTIRFM) data

Cold Spring Harbor Protocols

Volumn 7, Issue 6, 2012, Pages 722-725

  Beausang, John F ^a   Sun, Yujie ^a   Quinlan, Margot E ^a   Forkey, Joseph N ^a   Goldman, Yale E ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

MYOSIN V; ACTIN; FLUORESCENT DYE;

ACTIN FILAMENT; ARTICLE; ASSAY; CALIBRATION; CAMERA; DATA ANALYSIS; FLUORESCENCE MICROSCOPY; LIGHT INTENSITY; MOTILITY ASSAY; POLARIZED TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; INFORMATION PROCESSING; MACROMOLECULE; METABOLISM; METHODOLOGY; POLARIZATION MICROSCOPY; STAINING;

ACTINS; DATA COLLECTION; FLUORESCENT DYES; IMAGE PROCESSING, COMPUTER-ASSISTED; MACROMOLECULAR SUBSTANCES; MICROSCOPY, FLUORESCENCE; MICROSCOPY, POLARIZATION; MYOSIN TYPE V; STAINING AND LABELING;

EID: 84862139053     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot069419     Document Type: Article

References (4)

1. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012a. The polarized total internal reflection fluorescence microscopy (polTIRFM) processive motility assay for myosin V. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069393.
2. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012b. The polarized total internal reflection fluorescence microscopy (polTIRFM) twirling filament assay. Cold Spring Harb Protoc doi: 10.1101/pdb. prot069401.
3. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012c. Orien- tation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM). Cold Spring Harb Protoc doi: 10.1101/pdb.top069344.
4. Forkey JN, Quinlan ME, Goldman YE. 2005. Measurement of single mac- romolecule orientation by total internal reflection fluorescence polari- zation microscopy. Biophys J 89: 1261-1271.