Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme

PLoS ONE

Volumn 7, Issue 6, 2012, Pages

  Moser, Michael J ^a   DiFrancesco, Robert A ^a,b   Gowda, Krishne ^a   Klingele, Audrey J ^a   Sugar, Darby R ^a   Stocki, Stacy ^a   Mead, David A ^a   Schoenfeld, Thomas W ^a  

^a LUCIGEN CORPORATION   (United States)

^b ILLUMINA INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; MESSENGER RNA; PHOSPHODIESTERASE I; RNA DIRECTED DNA POLYMERASE; SPLEEN EXONUCLEASE; VIRUS RNA; DNA DIRECTED DNA POLYMERASE; TTH POLYMERASE;

ANIMAL CELL; ARTICLE; BIOCHEMISTRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; INFLUENZA VIRUS A; INTERMETHOD COMPARISON; METAGENOME; MOLONEY LEUKEMIA ONCOVIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; THERMUS THERMOPHILUS; VIRAL METAGENOME; WILD TYPE; COLIPHAGE; GENE LIBRARY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; TEMPERATURE; THERMAL SPRING; VIROLOGY; VIRUS;

DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; GENE LIBRARY; HOT SPRINGS; HUMANS; INFLUENZA A VIRUS; LEVIVIRUS; METAGENOME; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TEMPERATURE; VIRUSES;

EID: 84861909708     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038371     Document Type: Article

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