Subtyping botulinum neurotoxins by sequential multiple endoproteases in-gel digestion coupled with mass spectrometry

Analytical Chemistry

Volumn 84, Issue 11, 2012, Pages 4652-4658

  Wang, Dongxia ^a   Baudys, Jakub ^a   Rees, Jon ^a   Marshall, Kristin M ^b   Kalb, Suzanne R ^a   Parks, Bryan A ^a   Nowaczyk, Louis ^b   Pirkle, James L ^a   Barr, John R ^a  

^a NATIONAL CENTER FOR ENVIRONMENTAL HEALTH   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; BACTERIAL PATHOGENS; BOTULINUM NEUROTOXINS; CARROT JUICE; DIGESTION METHOD; ENZYMATIC DIGESTIONS; INGEL DIGESTION; PEPTIDE DETECTION; PEPTIDE IDENTIFICATION; PROTEIN SEQUENCES; PROTEOMICS; SEQUENCE SIMILARITY; SEROTYPES; SUBTYPINGS; TANDEM MASS SPECTROMETRY; TOXIC SUBSTANCES;

AMINO ACIDS; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PEPTIDES;

TOXIC MATERIALS;

BOTULINUM TOXIN; GUANIDINE; NEUROTOXIN; PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CLASSIFICATION; CLOSTRIDIUM BOTULINUM; GEL; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR TYPING; PROTEOMICS; SEQUENCE ALIGNMENT; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; BOTULINUM TOXINS; CHROMATOGRAPHY, LIQUID; CLOSTRIDIUM BOTULINUM; ENDOPEPTIDASES; GELS; GUANIDINE; MOLECULAR SEQUENCE DATA; MOLECULAR TYPING; NEUROTOXINS; PROTEOMICS; SEQUENCE ALIGNMENT; TANDEM MASS SPECTROMETRY;

EID: 84861888481     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac3006439     Document Type: Article

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