A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing

Microbiology and Immunology

Volumn 54, Issue 5, 2010, Pages 308-312

  Umeda, Kaoru ^a,b   Seto, Yoshiyuki ^b   Kohda, Tomoko ^b   Mukamoto, Masafumi ^b   Kozaki, Shunji ^b  

^a OSAKA CITY INSTITUTE OF PUBLIC HEALTH AND ENVIRONMENTAL SCIENCES   (Japan)

^b Osaka Prefecture University   (Japan)

Author keywords

BoNT A gene cluster typing; Infant botulism; Multiplex PCR; PFGE

Indexed keywords

BOTULINUM TOXIN A;

ARTICLE; BABY FOOD; BOTULISM; CLOSTRIDIUM BOTULINUM; CONTROLLED STUDY; FOOD CONTAMINATION; GENE CLUSTER; GENOTYPE; HONEY; JAPAN; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; SENSITIVITY AND SPECIFICITY;

BACTERIAL TYPING TECHNIQUES; BOTULINUM TOXIN TYPE A; BOTULISM; CLOSTRIDIUM BOTULINUM TYPE A; DNA, BACTERIAL; GENOTYPE; HUMANS; INFANT; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION;

CLOSTRIDIUM BOTULINUM;

EID: 77951750870     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2010.00213.x     Document Type: Article

References (17)

1. Cai S., Singh B.R., Sharma S. (2007) Botulism diagnostics: from clinical symptoms to in vitro assays. Crit Rev Microbiol 33: 109-25.
2. Lindstrom M., Korkeala H. (2006) Laboratory diagnostics of botulism. Clin Microbiol Rev 19: 298-314.
3. Hill K.K., Smith T.J., Helma C.H., Ticknor L.O., Foley B.T., Svensson R.T., Brown J.L., Johnson E.A., Smith L.A., Okinaka R.T., Jackson P.J., Marks J.D. (2007) Genetic diversity among botulinum neurotoxin producing Clostridial strains. J Bacteriol 189: 818-32.
4. Oguma K., Fujinaga Y., Inoue K. (1995) Structure and function of Clostridium botulinum toxins. Microbiol Immunol 39: 161-8.
5. Franciosa G., Floridi F., Maugliani A., Aureli P. (2004) Differentiation of the gene clusters encoding botulinum neurotoxin type A complexes in Clostridium botulinum type A, Ab, and A(B) strains. Appl Environ Microbiol 70: 7192-9.
6. Jacobson M.J., Lin G., Raphael B., Andreadis J., Johnson E.A. (2008) Analysis of neurotoxin cluster genes in Clostridium botulinum strains producing botulinum neurotoxin serotype A subtypes. Appl Environ Microbiol 74: 2778-86.
7. Noda H., Sugita K., Koike A., Nasu T., Takahashi M., Shimizu T., Ooi K., Sakaguchi G. (1988) Infant botulism in Asia. Am J Dis Child 142: 125-6.
8. Infectious Disease Surveillance Center, National Institute of Infectious Diseases. (2000) Botulism, Japan. Infectious Agents Surveillance Report (IASR) 21: 49-50. (in Japanese).
9. Infectious Disease Surveillance Center, National Institute of Infectious Diseases. (2008) Botulism in Japan as of January 2008. Infectious Agents Surveillance Report (IASR) 29: 35-36. (in Japanese).
10. Umeda K., Seto Y., Kohda T., Mukamoto M., Kozaki S. (2009) Genetic characterization of Clostridium botulinum associated with type B infant botulism in Japan. J Clin Microbiol 47: 2720-8.
11. Takeshi K., Fujinaga Y., Inoue K., Nakajima H., Oguma K., Ueno T., Sunagawa H., Ohyama T. (1996) Simple method for detection of Clostridium botulinum type A to F neurotoxin genes by polymerase chain reaction. Microbiol Immunol 40: 5-11.
12. Yamakawa K., Kamiya S., Nishida S., Yoshimura K., Yu H., Lu D.Y., Nakamura S. (1988) Distribution or Clostridium botulinum in Japan and in Shinkiang district of China. Microbiol Immunol 32: 579-87.
13. Brett M.M., McLauchlin J., Harris A., O'Brien S., Black N., Forsyth R.J., Roberts D., Bolton F.J. (2005) A case of infant botulism with a possible link to infant formula milk powder: evidence for the presence of more than one strain of Clostridium botulinum in clinical specimens and food. J Med Microbiol 54: 769-76.
14. Paerregaard A., Angen O., Lisby M., Molbak K., Clausen M.E., Christensen J.J. (2008) Denmark: botulism in an infant or infant botulism? Euro Surveill 13: pii:19072.
15. Nevas M., Lindstrom M., Virtanen A., Hielm S., Kuusi M., Arnon S.S., Vuori E., Korkeala H. (2005) Infant botulism acquired from household dust presenting as sudden infant death syndrome. J Clin Microbiol 43: 511-3.
16. Takeda Y., Toukubo Y., Inoue K., Ogawa H., Okada S., Sato T. (2000) Infant botulism by Clostridium botulinum possessing the type A and B botulism neurotoxin genes in Hiroshima prefecture. Jpn J Food Microbiol 17: 143-7. (in Japanese).
17. Kobayashi H., Fujisawa K., Saito Y., Kamijo M., Oshima S., Kubo M., Eto Y., Monma C., Kitamura M. (2003) A botulism case of a 12-year-old girl caused by intestinal colonization of Clostridium botulinum type Ab. Jpn J Infect Dis 56: 73-4.