Deletion of the single putative N-glycosylation site of the porcine circovirus type 2 Cap protein enhances specific immune responses by DNA immunisation in mice

Veterinary Journal

Volumn 192, Issue 3, 2012, Pages 385-389

  Gu, Jinyan ^a   Cao, Ruibin ^a   Zhang, Yu ^a   Lian, Xue ^a   Ishag, Hassan ^a   Chen, Puyan ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cap protein; DNA vaccines; Immune response; N linked glycosylation; Porcine circovirus type 2

Indexed keywords

CAP PROTEIN; DNA VACCINE; GAMMA INTERFERON; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2A; PLASMID DNA; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS VECTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ARTICLE; CD8+ T LYMPHOCYTE; CIRCOVIRUS; CIRCOVIRUS TYPE 2; CONTROLLED STUDY; DNA IMMUNIZATION; FEMALE; GENE DELETION; GENE SEQUENCE; IMMUNE RESPONSE; IMMUNOGENICITY; IMMUNOMODULATION; LYMPHOCYTE PROLIFERATION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN LOCALIZATION; WILD TYPE;

ANIMALS; CAPSID PROTEINS; CELL LINE; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; CYTOKINES; FEMALE; GENE EXPRESSION REGULATION; GLYCOSYLATION; LYMPHOCYTES; MICE; MICE, INBRED BALB C; PROTEIN FOLDING; SPLEEN; SWINE; VACCINES, DNA; VIRAL VACCINES;

EUKARYOTA; MUS; PORCINE CIRCOVIRUS 2;

EID: 84861886203     PISSN: 10900233     EISSN: 15322971     Source Type: Journal    
DOI: 10.1016/j.tvjl.2011.08.005     Document Type: Article

References (19)

1. Anders B., Hinkula J., Rowcliffe E., Biller M., Wahren B., Olofsson S. Enhanced immunogenicity of a human immunodeficiency virus type 1 env DNA vaccine by manipulating N-glycosylation signals effects of elimination of the V3 N306 glycan. Vaccine 2002, 20:397-405.
2. Beyene A., Basu A.K., Meyer R.R. Influence of N-linked glycans on intracellular transport of hepatitis C virus E1 chimeric glycoprotein and its role in pseudotype virus infectivity. Virology 2004, 324:273-285.
3. Blanchard P., Mahe D., Cariolet R., Keranflec'h A., Baudouard M.A., Cordioli P., Albina E., Jestin A. Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins. Vaccine 2003, 21:4565-4575.
4. Botarelli P., Houlden B.A., Haigwood N.L., Servis C., Montagna D., Abrignani S. N-Glycosylation of HIV-gp120 may constrain recognition by T lymphocytes. Journal of Immunology 1991, 147:3128-3132.
5. Hamel A.L., Lin L.L., Nayar G.P. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Journal of Virology 1998, 72:5262-5267.
6. Harding J.C. The clinical expression and emergence of porcine circovirus 2. Veterinary Microbiology 2004, 98:131-135.
7. Lewis P.J., Babiuk L.A. DNA vaccines: A review. Advances in Virus Research 1999, 54:129-188.
8. Reitter J.N., Means R.E., Desrosiers R.C. A role for carbohydrates in immune evasion in AIDS. Nature Medicine 1998, 4:679-684.
9. Seder R.A., Hill A.V. Vaccines against intracellular infections requiring cellular immunity. Nature 2000, 406:793-798.
10. Segalés J., Allan G.M., Domingo M. Porcine circovirus diseases. Animal Health Research Reviews 2005, 6:119-142.
11. Segalés J., Domingo M., Chianini F., Majo N., Dominguez J., Darwich L., Mateu E. Immunosuppression in postweaning multisystemic wasting syndrome affected pigs. Veterinary Microbiology 2004, 98:151-158.
12. Shang S.B., Li Y.F., Guo J.Q., Wang Z.T., Chen Q.X., Shen H.G., Zhou J.Y. Development and validation of a recombinant capsid protein-based ELISA for detection of antibody to porcine circovirus type 2. Research in Veterinary Science 2008, 84:150-157.
13. Shen H.G., Zhou J.Y., Huang Z.Y., Guo J.Q., Xing G., He J.L., Yan Y., Gong L.Y. Protective immunity against porcine circovirus 2 by vaccination with ORF2-based DNA and subunit vaccines in mice. Journal of General Virology 2008, 89:1857-1865.
14. Stevenson L.S., Gilpin D.F., Douglas A., McNeilly F., McNair I., Adair B.M., Allan G.M. T lymphocyte epitope mapping of porcine circovirus type 2. Viral Immunology 2007, 20:389-397.
15. Tischer I., Gelderblom H., Vettermann W., Koch M.A. A very small porcine virus with circular single-stranded DNA. Nature 1982, 295:64-66.
16. Vigerust D.J., Shepherd V.L. Virus glycosylation: Role in virulence and immune interactions. Trends in Microbiology 2007, 15:211-218.
17. Zhou J.Y., Chen Q.X., Ye J.X., Shen H.G., Chen T.F., Shang S.B. Serological investigation and genomic characterization of PCV2 isolates from different geographic regions of Zhejiang province in China. Veterinary Research Communications 2006, 30:205-220.
18. Zhou J.Y., Shang S.B., Gong H., Chen Q.X., Wu J.X., Shen H.G., Chen T.F., Guo J.Q. In vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus type II without nuclear localization signal. Journal of Biotechnology 2005, 118:201-211.
19. Zhou J.Y., Wang J.Y., Chen J.G., Wu J.X., Gong H., Teng Q.Y., Guo J.Q., Shen H.G. Cloning, in vitro expression and bioactivity of duck interleukin-2. Molecular Immunology 2005, 42:589-598.