Identification of EFEMP2 as a serum biomarker for the early detection of colorectal cancer with lectin affinity capture assisted secretome analysis of cultured fresh tissues

Journal of Proteome Research

Volumn 11, Issue 6, 2012, Pages 3281-3294

  Yao, Ling ^a,b   Lao, Weifeng ^c   Zhang, Yan ^a,b   Tang, Xiaorong ^a,b   Hu, Xiaotong ^c   He, Chao ^c   Hu, Xiaofang ^a   Xu, Lisa X ^a  

^a School of Biomedical Engineering and Med X Research Institute   (China)

^b SHANGHAI JIAO TONG UNIVERSITY   (China)

^c ZHEJIANG UNIVERSITY   (China)

Author keywords

biomarker; colorectal cancer; EFEMP2; fresh tissue culture; lectin affinity capture; secretome

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; EFEMP2 PROTEIN; TUMOR MARKER; UNCLASSIFIED DRUG; EFEMP2 PROTEIN, HUMAN; GLYCOPROTEIN; LECTIN; PROTEOME; SCLEROPROTEIN;

ARTICLE; ASCENDING COLON; CANCER GRADING; CANCER STAGING; CELL VIABILITY; CLINICAL ARTICLE; COLORECTAL CANCER; CONTROLLED STUDY; DESCENDING COLON; DIAGNOSTIC ACCURACY; EARLY DIAGNOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GEL ELECTROPHORESIS; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LECTIN BINDING; LIQUID CHROMATOGRAPHY; MALE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RECTUM; SIGMOID; TANDEM MASS SPECTROMETRY; TRANSVERSE COLON; ADENOCARCINOMA; ADENOMA; ADULT; AFFINITY CHROMATOGRAPHY; AGED; BLOOD; CASE CONTROL STUDY; CHEMISTRY; COLORECTAL NEOPLASMS; ISOLATION AND PURIFICATION; MIDDLE AGED; SECRETION (PROCESS); TISSUE CULTURE TECHNIQUE; UPREGULATION;

ADENOCARCINOMA; ADENOMA; ADULT; AGED; CASE-CONTROL STUDIES; CHROMATOGRAPHY, AFFINITY; COLORECTAL NEOPLASMS; EARLY DETECTION OF CANCER; EXTRACELLULAR MATRIX PROTEINS; FEMALE; GLYCOPROTEINS; HUMANS; LECTINS; MALE; MIDDLE AGED; PROTEOME; TISSUE CULTURE TECHNIQUES; TUMOR MARKERS, BIOLOGICAL; UP-REGULATION;

EID: 84861830236     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr300020p     Document Type: Article

References (76)

1. Jemal, A.; Siegel, R.; Xu, J.; Ward, E. Cancer statistics CA Cancer J. Clin. 2010, 60 (5) 277-300
2. Kim, Y.; Lee, S.; Park, S.; Jeon, H.; Lee, W.; Kim, J. K.; Cho, M.; Kim, M.; Lim, J.; Kang, C. S.; Han, K. Gastrointestinal tract cancer screening using fecal carcinoembryonic antigen Ann. Clin. Lab. Sci. 2003, 33 (1) 32-8
3. Mercer, D. W.; Talamo, T. S. Multiple markers of malignancy in sera of patients with colorectal carcinoma: preliminary clinical studies Clin. Chem. 1985, 31 (11) 1824-8
4. Chan, C. C.; Fan, C. W.; Kuo, Y. B.; Chen, Y. H.; Chang, P. Y.; Chen, K. T.; Hung, R. P.; Chan, E. C. Multiple serological biomarkers for colorectal cancer detection Int. J. Cancer 2010, 126 (7) 1683-90
5. Locker, G. Y.; Hamilton, S.; Harris, J.; Jessup, J. M.; Kemeny, N.; Macdonald, J. S.; Somerfield, M. R.; Hayes, D. F.; Bast, R. C., Jr. ASCO 2006 update of recommendations for the use of tumor markers in gastrointestinal cancer J. Clin. Oncol. 2006, 24 (33) 5313-27
6. Crawford, N. P.; Colliver, D. W.; Galandiuk, S. Tumor markers and colorectal cancer: utility in management J. Surg. Oncol. 2003, 84 (4) 239-48
7. Kulasingam, V.; Diamandis, E. P. Proteomics analysis of conditioned media from three breast cancer cell lines: a mine for biomarkers and therapeutic targets Mol. Cell. Proteomics 2007, 6 (11) 1997-2011
8. Gronborg, M.; Kristiansen, T. Z.; Iwahori, A.; Chang, R.; Reddy, R.; Sato, N.; Molina, H.; Jensen, O. N.; Hruban, R. H.; Goggins, M. G.; Maitra, A.; Pandey, A. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach Mol. Cell. Proteomics 2006, 5 (1) 157-71
9. Lai, M. D.; Xue, H.; Li, B. J.; Zhang, J.; Wu, M. L.; Huang, Q.; Wu, Q.; Sheng, H. Q.; Wu, D. D.; Hu, J. W. Identification of Serum Biomarkers for Colorectal Cancer Metastasis Using a Differential Secretome Approach J. Proteome Res. 2010, 9 (1) 545-55
10. Yao, L.; Zhang, Y.; Chen, K. Y.; Hu, X. F.; Xu, L. S. X. Discovery of IL-18 As a Novel Secreted Protein Contributing to Doxorubicin Resistance by Comparative Secretome Analysis of MCF-7 and MCF-7/Dox PLoS One 2011, 6 (9) e24684
11. Kulasingam, V.; Diamandis, E. P. Tissue culture-based breast cancer biomarker discovery platform Int. J. Cancer 2008, 123 (9) 2007-12
12. Mueller, M. M.; Fusenig, N. E. Friends or foes-bipolar effects of the tumour stroma in cancer Nat. Rev. Cancer 2004, 4 (11) 839-49
13. Liotta, L. A.; Kohn, E. C. The microenvironment of the tumour-host interface Nature 2001, 411 (6835) 375-9
14. Celis, J. E.; Gromov, P.; Cabezon, T.; Moreira, J. M. A.; Ambartsumian, N.; Rank, F.; Gromova, I. Proteomic characterization of the interstitial fluid perfusing the breast tumor microenvironment: a novel resource for biomarker and therapeutic target discovery Mol. Cell. Proteomics 2004, 3 (4) 327-44
15. Zwickl, H.; Traxler, E.; Staettner, S.; Parzefall, W.; Grasl-Kraupp, B.; Karner, J.; Schulte-Hermann, R.; Gerner, C. A novel technique to specifically analyze the secretome of cells and tissues Electrophoresis 2005, 26 (14) 2779-85
16. Shi, H. J.; Stubbs, R.; Hood, K. Characterization of de novo synthesized proteins released from human colorectal tumour explants Electrophoresis 2009, 30 (14) 2442-53
17. Zhang, Y.; Tang, X.; Yao, L.; Chen, K.; Jia, W.; Hu, X.; Xu, L. X. Lectin capture strategy for effective analysis of cell secretome Proteomics 2012, 12 (1) 32-6
18. Becker, J. W.; Reeke, G. N., Jr.; Wang, J. L.; Cunningham, B. A.; Edelman, G. M. The covalent and three-dimensional structure of concanavalin A. III. Structure of the monomer and its interactions with metals and saccharides J. Biol. Chem. 1975, 250 (4) 1513-24
19. Bhavanandan, V. P.; Katlic, A. W. The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid J. Biol. Chem. 1979, 254 (10) 4000-8
20. Keller, A.; Nesvizhskii, A. I.; Kolker, E.; Aebersold, R. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search Anal. Chem. 2002, 74 (20) 5383-92
21. Nesvizhskii, A. I.; Keller, A.; Kolker, E.; Aebersold, R. A statistical model for identifying proteins by tandem mass spectrometry Anal. Chem. 2003, 75 (17) 4646-58
22. Beissbarth, T.; Hyde, L.; Smyth, G. K.; Job, C.; Boon, W. M.; Tan, S. S.; Scott, H. S.; Speed, T. P. Statistical modeling of sequencing errors in SAGE libraries Bioinformatics 2004, 20 (Suppl. 1) i31-9
23. Old, W. M.; Meyer-Arendt, K.; Aveline-Wolf, L.; Pierce, K. G.; Mendoza, A.; Sevinsky, J. R.; Resing, K. A.; Ahn, N. G. Comparison of label-free methods for quantifying human proteins by shotgun proteomics Mol. Cell. Proteomics 2005, 4 (10) 1487-502
24. Bendtsen, J. D.; Nielsen, H.; von Heijne, G.; Brunak, S. Improved prediction of signal peptides: SignalP 3.0 J. Mol. Biol. 2004, 340 (4) 783-95
25. Nielsen, H.; Krogh, A. Prediction of signal peptides and signal anchors by a hidden Markov model Proc. Int. Conf. Intell. Syst. Mol. Biol. 1998, 6, 122-30
26. Welinder, C.; Ekblad, L. Coomassie staining as loading control in Western blot analysis J. Proteome Res. 2011, 10 (3) 1416-9
27. Morris, C. D.; Armstrong, G. R.; Bigley, G.; Green, H.; Attwood, S. E. Cyclooxygenase-2 expression in the Barrett's metaplasia-dysplasia-adenocarcinoma sequence Am. J. Gastroenterol. 2001, 96 (4) 990-6
28. Lis, H.; Sharon, N. Protein glycosylation. Structural and functional aspects Eur. J. Biochem./FEBS 1993, 218 (1) 1-27
29. Choi, D. S.; Lee, J. M.; Park, G. W.; Lim, H. W.; Bang, J. Y.; Kim, Y. K.; Kwon, K. H.; Kwon, H. J.; Kim, K. P.; Gho, Y. S. Proteomic analysis of microvesicles derived from human colorectal cancer cells J. Proteome Res. 2007, 6 (12) 4646-55
30. Hart, G. W.; Haltiwanger, R. S.; Holt, G. D.; Kelly, W. G. Glycosylation in the nucleus and cytoplasm Annu. Rev. Biochem. 1989, 58, 841-74
31. Batista, B. S.; Eng, W. S.; Pilobello, K. T.; Hendricks-Munoz, K. D.; Mahal, L. K. Identification of a conserved glycan signature for microvesicles J. Proteome Res. 2011, 10 (10) 4624-33
32. Kitahara, O.; Furukawa, Y.; Tanaka, T.; Kihara, C.; Ono, K.; Yanagawa, R.; Nita, M. E.; Takagi, T.; Nakamura, Y.; Tsunoda, T. Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser-capture microdissection of tumor tissues and normal epithelia Cancer Res. 2001, 61 (9) 3544-9
33. Takemasa, I.; Higuchi, H.; Yamamoto, H.; Sekimoto, M.; Tomita, N.; Nakamori, S.; Matoba, R.; Monden, M.; Matsubara, K. Construction of preferential cDNA microarray specialized for human colorectal carcinoma: molecular sketch of colorectal cancer Biochem. Biophys. Res. Commun. 2001, 285 (5) 1244-9
34. Birkenkamp-Demtroder, K.; Christensen, L. L.; Olesen, S. H.; Frederiksen, C. M.; Laiho, P.; Aaltonen, L. A.; Laurberg, S.; Sorensen, F. B.; Hagemann, R.; TF, O. R. Gene expression in colorectal cancer Cancer Res. 2002, 62 (15) 4352-63
35. Ichikawa, Y.; Ishikawa, T.; Takahashi, S.; Hamaguchi, Y.; Morita, T.; Nishizuka, I.; Yamaguchi, S.; Endo, I.; Ike, H.; Togo, S.; Oki, S.; Shimada, H.; Kadota, K.; Nakamura, S.; Goto, H.; Nitanda, H.; Satomi, S.; Sakai, T.; Narita, I.; Gejyo, F.; Tomaru, Y.; Shimizu, K.; Hayashizaki, Y.; Okazaki, Y. Identification of genes regulating colorectal carcinogenesis by using the algorithm for diagnosing malignant state method Biochem. Biophys. Res. Commun. 2002, 296 (2) 497-506
36. Lin, Y. M.; Furukawa, Y.; Tsunoda, T.; Yue, C. T.; Yang, K. C.; Nakamura, Y. Molecular diagnosis of colorectal tumors by expression profiles of 50 genes expressed differentially in adenomas and carcinomas Oncogene 2002, 21 (26) 4120-8
37. Agrawal, D.; Chen, T. G.; Irby, R.; Quackenbush, J.; Chambers, A. F.; Szabo, M.; Cantor, A.; Coppola, D.; Yeatman, T. J. Osteopontin identified as lead marker of colon cancer progression, using pooled sample expression profiling J. Nat. Cancer Inst. 2002, 94 (7) 513-21
38. Kwong, K. Y.; Bloom, G. C.; Yang, I.; Boulware, D.; Coppola, D.; Haseman, J.; Chen, E.; McGrath, A.; Makusky, A. J.; Taylor, J.; Steiner, S.; Zhou, J.; Yeatman, T. J.; Quackenbush, J. Synchronous global assessment of gene and protein expression in colorectal cancer progression Genomics 2005, 86 (2) 142-58
39. Koehler, A.; Bataille, F.; Schmid, C.; Ruemmele, P.; Waldeck, A.; Blaszyk, H.; Hartmann, A.; Hofstaedter, F.; Dietmaier, W. Gene expression profiling of colorectal cancer and metastases divides tumours according to their clinicopathological stage J. Pathol. 2004, 204 (1) 65-74
40. Gardina, P. J.; Clark, T. A.; Shimada, B.; Staples, M. K.; Yang, Q.; Veitch, J.; Schweitzer, A.; Awad, T.; Sugnet, C.; Dee, S.; Davies, C.; Williams, A.; Turpaz, Y. Alternative splicing and differential gene expression in colon cancer detected by a whole genome exon array BMC Genom. 2006, 7, 325
41. Conrotto, P.; Roesli, C.; Rybak, J.; Kischel, P.; Waltregny, D.; Neri, D.; Castronovo, V. Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis Int. J. Cancer 2008, 123 (12) 2856-64
42. Kim, H. J.; Kang, H. J.; Lee, H.; Lee, S. T.; Yu, M. H.; Kim, H.; Lee, C. Identification of S100A8 and S100A9 as Serological Markers for Colorectal Cancer J. Proteome Res. 2009, 8 (3) 1368-79
43. Volmer, M. W.; Stuhler, K.; Zapatka, M.; Schoneck, A.; Klein-Scory, S.; Schmiegel, W.; Meyer, H. E.; Schwarte-Waldhoff, I. Differential proteome analysis of conditioned media to detect Smad4 regulated secreted biomarkers in colon cancer Proteomics 2005, 5 (10) 2587-601
44. Alfonso, P.; Nunez, A.; Madoz-Gurpide, J.; Lombardia, L.; Sanchez, L.; Casal, J. I. Proteomic expression analysis of colorectal cancer by two-dimensional differential gel electrophoresis Proteomics 2005, 5 (10) 2602-11
45. Friedman, D. B.; Hill, S.; Keller, J. W.; Merchant, N. B.; Levy, S. E.; Coffey, R. J.; Caprioli, R. M. Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry Proteomics 2004, 4 (3) 793-811
46. Bi, X.; Lin, Q.; Foo, T. W.; Joshi, S.; You, T.; Shen, H. M.; Ong, C. N.; Cheah, P. Y.; Eu, K. W.; Hew, C. L. Proteomic analysis of colorectal cancer reveals alterations in metabolic pathways: mechanism of tumorigenesis Mol. Cell Proteomics 2006, 5 (6) 1119-30
47. Stulik, J.; Hernychova, L.; Porkertova, S.; Knizek, J.; Macela, A.; Bures, J.; Jandik, P.; Langridge, J. I.; Jungblut, P. R. Proteome study of colorectal carcinogenesis Electrophoresis 2001, 22 (14) 3019-25
48. Zhang, Y.; Ye, Y.; Shen, D.; Jiang, K.; Zhang, H.; Sun, W.; Zhang, J.; Xu, F.; Cui, Z.; Wang, S. Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis Cancer Sci. 2010, 101 (2) 523-9
49. Schwarte-Waldhoff, I.; Diehl, H. C.; Stuhler, K.; Klein-Scory, S.; Volmer, M. W.; Schoneck, A.; Bieling, C.; Schmiegel, W.; Meyer, H. E. A catalogue of proteins released by colorectal cancer cells in vitro as an alternative source for biomarker discovery Proteomics Clin. Appl. 2007, 1 (1) 47-61
50. Fong, S. F. T.; Dietzsch, E.; Fong, K. S. K.; Hollosi, P.; Asuncion, L.; He, Q. P.; Parker, M. I.; Cziszar, K. Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors Genes Chromosomes Cancer 2007, 46 (7) 644-55
51. Troy, A. M.; Sheahan, K.; Mulcahy, H. E.; Duffy, M. J.; Hyland, J. M. P.; O'Donoghue, D. P. Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression Eur. J. Cancer 2004, 40 (10) 1610-16
52. Sakakibara, T.; Hibi, K.; Koike, M.; Fujiwara, M.; Kodera, Y.; Ito, K.; Nakao, A. Plasminogen activator inhibitor-1 as a potential marker for the malignancy of colorectal cancer Br. J. Cancer 2005, 93 (7) 799-803
53. Bergeron, S.; Lemieux, E.; Durand, V.; Cagnol, S.; Carrier, J. C.; Lussier, J. G.; Boucher, M. J.; Rivard, N. The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis Mol. Cancer 2010, 9, 271
54. Timpl, R.; Sasaki, T.; Kostka, G.; Chu, M. L. Fibulins: a versatile family of extracellular matrix proteins Nat. Rev. Mol. Cell. Biol. 2003, 4 (6) 479-89
55. Argraves, W. S.; Greene, L. M.; Cooley, M. A.; Gallagher, W. M. Fibulins: physiological and disease perspectives EMBO Rep. 2003, 4 (12) 1127-31
56. de Vega, S.; Iwamoto, T.; Nakamura, T.; Hozumi, K.; McKnight, D. A.; Fisher, L. W.; Fukumoto, S.; Yamada, Y. TM14 is a new member of the fibulin family (fibulin-7) that interacts with extracellular matrix molecules and is active for cell binding J. Biol. Chem. 2007, 282 (42) 30878-88
57. Argraves, W. S., Fibulins. In Guidebook to the Extracellular Matrix, Anchor and Adhesion Proteins, 2nd ed.; Vale, T. K. R., Ed. Oxford University Press: Oxford, 1999; pp 425-30.
58. Gallagher, W. M.; Greene, L. M.; Ryan, M. P.; Sierra, V.; Berger, A.; Laurent-Puig, P.; Conseiller, E. Human fibulin-4: analysis of its biosynthetic processing and mRNA expression in normal and tumour tissues FEBS Lett. 2001, 489 (1) 59-66
59. Forti, S.; Scanlan, M. J.; Invernizzi, A.; Castiglioni, F.; Pupa, S.; Agresti, R.; Fontanelli, R.; Morelli, D.; Old, L. J.; Pupa, S. M.; Menard, S. Identification of breast cancer-restricted antigens by antibody screening of SKBR3 cDNA library using a preselected patient's serum Breast Cancer Res. Treat. 2002, 73 (3) 245-56
60. Tahara, K.; Mimori, K.; Iinuma, H.; Iwatsuki, M.; Yokobori, T.; Ishii, H.; Anai, H.; Kitano, S.; Mori, M. Serum Matrix-Metalloproteinase-1 is a Bona Fide Prognostic Marker for Colorectal Cancer Ann. Surg. Oncol. 2010, 17 (12) 3362-69
61. Kemik, O.; Kemik, A. S.; Sumer, A.; Dulger, A. C.; Adas, M.; Begenik, H.; Hasirci, I.; Yilmaz, O.; Purisa, S.; Kisli, E.; Tuzun, S.; Kotan, C. Levels of matrix metalloproteinase-1 and tissue inhibitors of metalloproteinase-1 in gastric cancer World J. Gastroenterol. 2011, 17 (16) 2109-12
62. Li, M.; Xiao, T.; Zhang, Y.; Feng, L.; Lin, D.; Liu, Y.; Mao, Y.; Guo, S.; Han, N.; Di, X.; Zhang, K.; Cheng, S.; Gao, Y. Prognostic significance of matrix metalloproteinase-1 levels in peripheral plasma and tumour tissues of lung cancer patients Lung Cancer 2010, 69 (3) 341-7
63. Nikkola, J.; Vihinen, P.; Vuoristo, M. S.; Kellokumpu-Lehtinen, P.; Kahari, V. M.; Pyrhonen, S. High serum levels of matrix metalloproteinase-9 and matrix metalloproteinase-1 are associated with rapid progression in patients with metastatic melanoma Clin. Cancer Res. 2005, 11 (14) 5158-66
64. Herszenyi, L.; Farinati, F.; Cardin, R.; Istvan, G.; Molnar, L. D.; Hritz, I.; De Paoli, M.; Plebani, M.; Tulassay, Z. Tumor marker utility and prognostic relevance of cathepsin B, cathepsin L, urokinase-type plasminogen activator, plasminogen activator inhibitor type-1, CEA and CA 19-9 in colorectal cancer BMC Cancer 2008, 8, 194
65. Berger, D. H. Plasmin/plasminogen system in colorectal cancer World J. Surg. 2002, 26 (7) 767-71
66. Iwadate, Y.; Hayama, M.; Adachi, A.; Matsutani, T.; Nagai, Y.; Hiwasa, T.; Saeki, N. High serum level of plasminogen activator inhibitor-1 predicts histological grade of intracerebral gliomas Anticancer Res. 2008, 28 (1B) 415-8
67. Strojan, P.; Budihna, M.; Smid, L.; Vrhovec, I.; Skrk, J. Urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) in tissue and serum of head and neck squamous cell carcinoma patients Eur. J. Cancer 1998, 34 (8) 1193-7
68. Rosenzweig, C. N.; Zhang, Z.; Sun, X.; Sokoll, L. J.; Osborne, K.; Partin, A. W.; Chan, D. W. Predicting Prostate Cancer Biochemical Recurrence Using a Panel of Serum Proteomic Biomarkers J. Urol. 2009, 181 (3) 1407-14
69. Bernacka, K.; Kuryliszyn-Moskal, A.; Sierakowski, S. The levels of alpha 1-antitrypsin and alpha 1-antichymotrypsin in the sera of patients with gastrointestinal cancers during diagnosis Cancer 1988, 62 (6) 1188-93
70. Wang, M.; Long, R. E.; Comunale, M. A.; Junaidi, O.; Marrero, J.; Di Bisceglie, A. M.; Block, T. M.; Mehta, A. S. Novel fucosylated biomarkers for the early detection of hepatocellular carcinoma Cancer Epidemiol. Biomarkers Prev. 2009, 18 (6) 1914-21
71. Hamrita, B.; Chahed, K.; Trimeche, M.; Guillier, C. L.; Hammann, P.; Chaieb, A.; Korbi, S.; Chouchane, L. Proteomics-based identification of alpha1-antitrypsin and haptoglobin precursors as novel serum markers in infiltrating ductal breast carcinomas Clin. Chim. Acta 2009, 404 (2) 111-8
72. Li, Y.; Krowka, M. J.; Qi, Y.; Katzmann, J. A.; Song, Y.; Mandrekar, S. J.; Yang, P. Alpha1-antitrypsin deficiency carriers, serum alpha 1-antitrypsin concentration, and non-small cell lung cancer survival J. Thorac. Oncol. 2011, 6 (2) 291-5
73. El-Akawi, Z. J.; Abu-Awad, A. M.; Sharara, A. M.; Khader, Y. The importance of alpha-1 antitrypsin (alpha1-AT) and neopterin serum levels in the evaluation of non-small cell lung and prostate cancer patients Neuroendocrinol. Lett. 2010, 31 (1) 113-6
74. Alexandrakis, M. G.; Passam, F. H.; Ganotakis, E. S.; Sfiridaki, K.; Xilouri, I.; Perisinakis, K.; Kyriakou, D. S. The clinical and prognostic significance of erythrocyte sedimentation rate (ESR), serum interleukin-6 (IL-6) and acute phase protein levels in multiple myeloma Clin. Lab. Haematol. 2003, 25 (1) 41-6
75. Gallagher, W. M.; Argentini, M.; Sierra, V.; Bracco, L.; Debussche, L.; Conseiller, E. MBP1: a novel mutant p53-specific protein partner with oncogenic properties Oncogene 1999, 18 (24) 3608-16
76. Heine, H.; Delude, R. L.; Monks, B. G.; Espevik, T.; Golenbock, D. T. Bacterial lipopolysaccharide induces expression of the stress response genes hop and H411 J. Biol. Chem. 1999, 274 (30) 21049-55